An electron microscope study of the heat denaturation of human serum albumin in solutions
The heat denaturation of human serum albumin has been studied by a number of authors (1–5). In most cases, indirect methods have been used to derive information about the mechanism of aggregation of the albumin particles by heat, and the products of this process. Since the system of particles formed by aggregation as a result of heat-induced denaturation is extremely complex it may be anticipated that the interpretation of the results obtained by indirect methods will be rather difficult. For this reason, we decided to investigate this problem by electron microscopy. We were, of course, quite aware that these results, too, would be subject to certain errors, mainly because of some of the conditions involved in the preparation of the samples. In the first stages of this work, it was our aim to get preliminary information about the basic features of this process.
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