Advertisement

Verhandlungen pp 903-906 | Cite as

A water-miscible embedding resin for electron microscopy

  • I. R. Gibbons

Abstract

Embedding media that are miscible with water have played a significant role in light microscopy, particularly in the study of lipids. The embedding media used for this purpose have mostly been based upon polyethylene glycols (1); they do not have suitable properties for cutting the specially thin sections needed for electron microscopy. On the other hand, the embedding media that have been developed for thin sectioning are not miscible with water and have necessitated treatment of the specimens with a separate dehydrating agent, such as acetone or ethyl alcohol, before embedding (2, 3, 4). Gelatin has been proposed as a water-miscible embedding medium for electron microscopy (5, 6, 7), but it seems likely that the great shrinkage that occurs during hardening will preclude its general use.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Miles, A. E. W., and J. E. Lindner: J. roy. Mier. Soc. 72, 199 (1952).CrossRefGoogle Scholar
  2. 2.
    Newman, S. B., E. Borysko and M. Swerdlow: J. Res. nat. Bur. Stand. 43, 183 (1949).CrossRefGoogle Scholar
  3. 3.
    Glauert, A. M., and R. H. Glauert: J. biophys. biochem. Cytol. 4, 191 (1958).CrossRefGoogle Scholar
  4. 4.
    Kellenberger, E., W. Schwab and A. Ryter: Experientia (Basel) 12, 421 (1956).Google Scholar
  5. 5.
    Gilëv, V. P.: Electron microscopy: Proceedings of the Stockholm Congress. p. 113. Stockholm Almqvist and Wiksell 1957.Google Scholar
  6. 6.
    Gilëv, V. P.: J. Ultrastructural Res. 1, 349 (1958).Google Scholar
  7. 7.
    Fernandez-Moran, H., and J. B. Finean: J. biophys. biochem. Cytol. 3, 725 (1957).CrossRefGoogle Scholar
  8. 8.
    Lee, H., and K. Neville: Epoxy resins. New York: McGraw Hill 1957.Google Scholar
  9. 9.
    Gibbons, I. R., and J. R. G. Bradfield: Electron Microscopy: Proceedings of the Stockholm Congress. p. 121. Stockholm: Almqvist and Wiksell 1957.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1960

Authors and Affiliations

  • I. R. Gibbons
    • 1
  1. 1.Biological LaboratoriesHarvard UniversityCambridge 38USA

Personalised recommendations