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Deep Segmentation of Bacteria at Different Stages of the Life Cycle

  • Roman SpilgerEmail author
  • Tobias Schwackenhofer
  • Charlotte Kaspar
  • Ilka Bischofs
  • Karl Rohr
Conference paper
  • 55 Downloads
Part of the Informatik aktuell book series (INFORMAT)

Zusammenfassung

Segmentation of bacteria in live cell microscopy image sequences is a crucial task to gain insights into molecular processes. A main challenge is that some bacteria strongly change their appearance during the life cycle as response to fluctuations in environmental conditions. We present a novel deep learning method with shape-based weighting of the loss function to accurately segment bacteria during different stages of the life cycle. We evaluate the performance of the method for live cell microscopy images of Bacillus subtilis bacteria with strong changes during the life cycle.

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Copyright information

© Springer Fachmedien Wiesbaden GmbH, ein Teil von Springer Nature 2020

Authors and Affiliations

  • Roman Spilger
    • 1
    Email author
  • Tobias Schwackenhofer
    • 1
  • Charlotte Kaspar
    • 2
    • 3
  • Ilka Bischofs
    • 2
    • 3
  • Karl Rohr
    • 1
  1. 1.Biomedical Computer Vision Group, BioQuant, IPMBHeidelberg University Im Neuenheimer Feld 267HeidelbergDeutschland
  2. 2.BioQuant, Center for Molecular Biology (ZMBH),Heidelberg UniversityHeidelbergDeutschland
  3. 3.Max Planck Institute for Terrestrial MicrobiologyMarburgDeutschland

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