Detection of Coronaviruses by the Polymerase Chain Reaction
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A simple and reliable method for the amplification and specific detection of human coronavirus nucleotide sequences was developed, based on the synthesis of cDNA, the polymerase chain reaction, and the use of oligonucleotide probes in Southern blots. Regions from several genes of the two prototype strains of human coronavirus (229E and OC43) could be specifically amplified. This powerful technique was applied to clinical specimens, with appropriate controls, to study the tissue tropism of coronaviruses and their possible involvement in diseases other than the common cold. We have obtained preliminary evidence for the detection of the genome of a human coronavirus in central nervous system autopsy tissue from some multiple sclerosis patients.
KeywordsMultiple Sclerosis Patient Myelin Basic Protein Infectious Bronchitis Virus Rubella Virus Equine Arteritis Virus
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