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Noninstrumental Detection of Influenza Viruses by Enzyme Immunoassay

  • R. Pottathil
  • M. W. Harmon
  • L. Keys
  • K. Desai
Chapter
  • 137 Downloads

Abstract

Influenza viruses are usually associated with epidemic respiratory infections in all age groups (Glenzen 1982; Kendal and Walls 1987). Influenza A and B produce symptoms in humans that are not pathognomonic. During epidemics, the infection may be diagnosed on a clinical basis. However, isolated cases, as well as patients seen at the beginning of an outbreak between epidemics, are difficult to diagnose clinically. A series of infectious agents such as respiratory syncytial virus, adenovirus, corona virus, rhinovirus, the parainfluenza virus group, and Mycoplasma pneumonia can produce the clinical symptoms often designated as “the flu” (Huley 1987). Definitive diagnosis of influenza virus infection has traditionally relied on viral isolation or serology. However, the time required for laboratory diagnosis has often exceeded the duration of the disease. These delayed laboratory reports coupled with the lack of specific therapy resulted in decreased interest in obtaining a laboratory diagnosis. However, the recent availability of specific antiviral agents and rapid diagnostic techniques have revived the interest in laboratory diagnosis.

Keywords

Influenza Virus Respiratory Syncytial Virus Latex Particle Laboratory Diagnosis Weak Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Barker WH, Mullooly JP (1980) Impact of epidemic type A influenza in a defined adult population. Am J Epidemol 112: 798–811Google Scholar
  2. Berg RA, Yolken RH, Rennard SI, Dolin R, Murphy BR, Straus SE (1980) New immunoassay for measurement of influenza A/Victoria/3/75 virus in nasal washes. Lancet 1: 851–853PubMedCrossRefGoogle Scholar
  3. Epsy MJ, Smith TF, Harmon MW, Kendal AP (1986) Rapid detection of influenza virus by shell vial assay with monoclonal antibodies. J Clin Microbiol 24: 677–679Google Scholar
  4. Glezen WP (1982) Serious morbidity and mortality associated with influenza epidemics. Epidemiol Rev 4: 25–44PubMedGoogle Scholar
  5. Halonen P, Obert G, Heirhulzer JC (1985) Direct detection of viral antigens in respiratory infections by immunoassays: a four year experience and new developments. Med Virol IV, Hillsdale, NJ, Lawrence Erbaum Associates, pp 65–83Google Scholar
  6. Harmon MW, Pawlik KM (1982) Enzyme immunoassay for direct detection of influenza type A nand adenovirus antigens in clinical specimen. J Clin Microbiol 15: 5–11PubMedGoogle Scholar
  7. Hayden FG (1987) Amantidine and rimantidine: efficacy, side effects, pharmacokinetics. J Res Dis 8: S45 - S50Google Scholar
  8. Huley PH (1987) Diagnostic testing for influenza A. J Res Dis 8: S27 - S31Google Scholar
  9. Kendal AP, Walls HH (1987) Epidimeology of influenza and potential for resistant strains. J Resp Dis 8:S 15–26Google Scholar
  10. Oxford JS, Galbraith AW (1980) Antiviral activity of amantidine: a review of laboratory and clinical data. Pharmacol Ther 11: 181–262PubMedCrossRefGoogle Scholar
  11. Sarkkinen HK, Halonen PE, Salmi AA (1981) Detection of influenza A virus by radioimmunoassay and enzyme immunoassay from nasopharyngeal specimens. J Med Virol 7: 213–220PubMedCrossRefGoogle Scholar
  12. Shalit IP, McKee PA, Beauchamp H, Waner JL (1985) Comparison of polyclonal serum versus monoclonal antibodies for the rapid diagnosis of influenza A virus infections by immunofluorescence in clinical specimen. J Clin Microbiol 22: 877–879PubMedGoogle Scholar
  13. Walls HH, Harmon MW, Slagle JJ, Stocksdale C, Kendal AP (1986a) Characterization and evaluation of monoclonal antibodies developed for typing influenza A and influenza B strains. J Clin Microbiol 23: 240–245PubMedGoogle Scholar
  14. Walls HH, Johansson KH, Harmon MW, Halonen PE, Kendal AP (1986b) Time-resolved fluoro-immunoassay with monoclonal antibodies for rapid diagnosis of influenza infections. Clin Microbiol 24: 907–912Google Scholar
  15. Wingfield WL, Pollack D, Grunert RR (1969) Therapeutic efficacy of amantidine HC1 and rimantidine HC1 in naturally occurring influenza N2 respiratory illness in man. N Engl J Med 281: 579–584PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • R. Pottathil
    • 1
  • M. W. Harmon
    • 2
  • L. Keys
  • K. Desai
    • 2
  1. 1.Roche Diagnostic Systems, Inc.NutleyUSA
  2. 2.Center for Disease ControlAtlantaUSA

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