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ODMR Studies of Protein-Nucleic Acid Interactions

  • D. H. H. Tsao
  • A. H. Maki
Conference paper

Abstract

ODMR and phosphorescence measurements on complexes of E. Coli single-stranded DNA binding proteins bearing specific tryptophan (W) → phenylalanine mutation with single-stranded poly (dT) and poly (2-thiouracil) have been made. They demonstrate that close-range interactions of W40 and W54 occur with the bases of the polynuclpeotide. At least in the case of W54 in the (dT) complex, an aromatic stacking interaction involving charge-transfer appears to occur.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • D. H. H. Tsao
    • 1
  • A. H. Maki
    • 1
  1. 1.Department of ChemistryUniversity of CaliforniaDavisUSA

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