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Fast Electron Transfer Kinetics in Photosystem I from Transient EPR-Spectrosoopy at Room Temperature

  • I. Sieckmann
  • C. Bock
  • K. Brettel
  • A. van der Est
  • P. Sétif
  • D. Stehlik
Conference paper

Abstract

Following light excitation of the donor P700 and primary charge separation, electron transfer in photosystem I (PSI) proceeds via a chain of membrane bound acceptors termed A0, A1 Fx, FA, FB. Transient EPR-spectroscopy of spin polarized signals provides a well suited method to study structural and functional aspects of these processes with a resolution presently approaching the physical limit around 10 ns. Recently we showed that in intact PS I preparations at ambient temperatures the primary sub-nanosecond events are followed by the electron transfer step P+ 700(FeS) P+ 700A- 1(FeS) P+ 700A1(FeS), where (FeS) represents the first iron sulfur center (possibly Fx) 1).

References

  1. 1).
    Bock. C., van der Est. A.J.. Brettel. K., Stehlik. D. (1989) FEBS Lett. 247 91–96CrossRefGoogle Scholar
  2. 2).
    Brettel, K. (1989) Biochim. Biophys. Acta 976 246–249CrossRefGoogle Scholar
  3. 3).
    Brettel. K.. Setif. P. and Mathis. P. (1986) FEBS Lett. 203 220–224CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • I. Sieckmann
    • 1
  • C. Bock
    • 1
  • K. Brettel
    • 1
    • 2
  • A. van der Est
    • 1
  • P. Sétif
    • 1
    • 3
  • D. Stehlik
    • 1
  1. 1.Fachbereich PhysikFreie Universität BerlinBerlin 33Germany
  2. 2.Max Volmer InstitutTechnische Universität BerlinBerlin 12Germany
  3. 3.Departement de Biologie CEN de SaclayService de BiophysiqueGif-sur-YvetteFrance

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