The Ribosomal Frame-Shift Signal of Infectious Bronchitis Virus
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NATO ASI Series
book series (volume 49)
We recently described the first non-retroviral example of ribosomal frame-shifting in higher eukaryotes (Brierley et al., 1987). The shift occurs during translation of the genomic RNA of the coronavirus infectious bronchitis virus (IBV), just at the end of the F1 open reading frame (located at the 5′ end of the genome); its consequence is that a proportion of ribosomes reading the F1 frame fail to terminate at the F1 stop codon, and instead begin reading the F2 open reading frame (ORF), which overlaps the end of F1, leading to the production of an F1–F2 fusion protein. This “-1” frame-shift is highly efficient (about 30%) and can be reproduced in vitro by cloning a short sequence from the junction of the F1/F2 ORFs into a suitable reporter gene (Figure 1). The recombinant gene may then be transcribed using the phage T7 RNA polymerase, and the resulting mRNA translated in the rabbit reticulocyte lysate cell-free system.
KeywordsInfectious Bronchitis Virus Rous Sarcoma Virus Pseudoknot Structure Ribosomal Frameshifting Slippery Sequence
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