Alternative Translation and Functional Diversity of Release Factor 2 and Lysyl-tRNA Synthetase

  • Y. Nakamura
  • K. Kawakami
  • O. Mikuni
Conference paper
Part of the NATO ASI Series book series (volume 49)


The prfB gene encodes pep tide-chain-release factor 2 which catalyzes translation termination at nonsense codons UGA and UAA in bacteria. Mutations in prfB cause misreading of UGA, i.e., increased frameshift or suppression. These translational errors were due to inefficient translation termination at UGA. As one extreme case, an opal (UGA) mutation in prfB is autogenously suppressed. The prfB gene is in the same operon with herC, a gene defined by a suppressor mutation of a replication-defective ColEl plasmid. The herC gene coincides with lysS, a gene encoding a major species of lysyl-tRNA synthetase. Thus, the genetic organization of prfB and lysS in the same operon may suggest structural, functional and evolutional relevance of these gene products.


Suppressor Mutation Tryptophan Operon Stop Codon Recognition Diadenosine Tetraphosphate Plasmid pKK951 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Y. Nakamura
    • 1
  • K. Kawakami
    • 1
  • O. Mikuni
    • 1
  1. 1.Department of Tumor Biology The Institute of Medical ScienceThe University of TokyoP.O. Takanawa, Tokyo 108Japan

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