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Flow Cytometry and Cell Sorting

  • Mack J. Fulwyler
Conference paper

Abstract

Flow cytometry has become an important research tool in cytology, genetics, immunology, and microbiology. The information gained from cytometric instruments is quantitative and of high statistical precision, enabling resolution of cell subpopulations.

Although increasing, application to cytology is hindered by inadequate appreciation of the nature of flow cytometry and the information obtained. Many cytologic questions can be reexamined from the perspective of this technology to obtain knowledge not accessible with conventional techniques.

A flow cytometer and cell sorter are described. The physical, biochemical, and functional properties measurable by these systems are discussed.

KEY WORDS

Flow cytometry Cell Sorting Cytology. 

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References

  1. 1.
    ARNDT-JOVIN, D. J., JOVIN, T. M.: Cell separation using fluorescence emission anisotropy. In: Membranes and Neoplasia: New Approaches and Strategies. New York, Alan R. Liss, 1976, pp. 123–136Google Scholar
  2. 2.
    ARNDT-JOVIN, D. J., JOVIN, T. M.: Analysis and sorting of living cells according to deoxyribonucleic acid content. J. Histochem. Cytochem. 25, 585, 1977 PubMedCrossRefGoogle Scholar
  3. 3.
    ARNDT-JOVIN, D. J., JOVIN, T. M.: Automated cell sorting with flow systems. Annu. Rev. Biophys. Bioeng. 7, 1978 Google Scholar
  4. 4.
    BIGBEE, W. L., BRANSCOMB, E. W., MENDELSOHN, M. L.: Detection of somatic mutations in human erythrocytes. In: Tenth Annual Meeting Environmental Mutagen Society, March 9 through 12, New Orleans 1979 Google Scholar
  5. 5.
    BOHN, B.: High sensitivity cytofluorometric quantitation of lectin and hormone binding to surfaces of living cells. Exp. Cell Res. 103, 39, 1976 PubMedCrossRefGoogle Scholar
  6. 6.
    CALLIS, J., HOEHN, H.: Flow fluorometric diagnosis of euploid and aneuploid human lymphocytes. Am. J. Hum. Genet. 28, 577, 1976 PubMedCentralPubMedGoogle Scholar
  7. 7.
    CHAN, S. S., ARNDT-JOVIN, D. J., JOVIN, T. M.: Proximity of lectin receptors on the cell surface measured by fluorescence energy transfer in a flow system. J. Histochem. Cytochem. 27, 56, 1979 PubMedCrossRefGoogle Scholar
  8. 8.
    COULTER, H. W.: High speed automatic blood cell counter and cell size analyzer. Proc. Natl. Electron. Conf. 12, 1034, 1956 Google Scholar
  9. 9.
    CRAM, L. S., FORSLUND, J. C., HORAN, J. K., STEINKAMP, J. A.: Application of flow microfluorometry (fmf) and cell sorting techniques to the control of animal diseases. In: Automation in Microbiology and Immunology. Wiley and Sons, 1975, pp. 51–60Google Scholar
  10. 10.
    CRAM, L. S., LEHMAN, J. M.: Flow microfluorometric DNA content measurements of tissue culture cells and peripheral lymphocytes. Hum. Genet. 37, 201, 1977 PubMedCrossRefGoogle Scholar
  11. 11.
    CRISSMAN, H. A., OKA, M. S., STEINKAMP, J. A.: Rapid staining methods for analysis of deoxyribonucleic acid and protein in mammalian cells. J. Histochem. Cytochem. 27, 64, 1979 CrossRefGoogle Scholar
  12. 12.
    DARZYNKIEWICZ, A., TRAGANOS, F., ANDREEFF, T., SHARPLESS, T., MELAMED, M. R.: Different sensitivity of chromatin to acid denaturation in quiescent and cycling cells revealed by flow cytometry. J. Histochem. Cytochem. 27, 478, 1979 PubMedCrossRefGoogle Scholar
  13. 13.
    DITTRICH, W., GOEHDE, W.: Impulsfluorometric bei Enzelzellen in Suspensionen. Z. Naturforsch. [B] 24, 360, 1969 Google Scholar
  14. 14.
    DOHERTY, R. A., HORAN, P. K.: Applications of rapid single cell analysis to perinatal medicine and pediatrics. Am. J. Dis. Child. 132, 556, 1978 PubMedGoogle Scholar
  15. 15.
    DOLBEARE, F. A., SMITH, R. E.: Flow cytoenzymology: Rapid enzyme analysis of single cells. In: MELAMED, MULLANEY, MENDELSOHN, eds., Flow Cytometry and Sorting. Wiley and Sons (in press) 1979 Google Scholar
  16. 16.
    FULWYLER, M. J.: Electronic separation of cells by volume. Science 150, 910, 1965 PubMedCrossRefGoogle Scholar
  17. 17.
    HORAN, P. K., WHEELESS, L. L.: Quantitative single cell analysis and sorting. Science 198, 149, 1977 PubMedCrossRefGoogle Scholar
  18. 18.
    JONGKIND, J. F., VERKERK, A., TANKE, H.: Isolation of human fibroblast heterokaryons with two-color flow sorting. Exp. Cell Res. (in press) 1979 Google Scholar
  19. 19.
    KAMENTSKY, L. A., MELAMED, M. R., DERMAN, H.: Spectrophotometer: New instrument for ultrarapid cell analysis. Science 150, 630, 1965 PubMedCrossRefGoogle Scholar
  20. 20.
    KRISHAN, A., PITMAN, S. W., TATTERSALL, M.H.N., PAIKA, K. D., SMITH, D.C., FREVI, E.: Flow microfluorometric patterns of human bone marrow and tumor cells in response to cancer chemotherapy. Cancer Res. 36, 3813, 1976 PubMedGoogle Scholar
  21. 21.
    LATT, S. A.: Fluorometric detection of deoxyribonucleic acid synthesis: Possibilities for interfacing bromodeoxyuridine dye techniques with flow fluorometry. J. Histochem. Cytochem. 25, 913, 1977 PubMedCrossRefGoogle Scholar
  22. 22.
    LEARY, J. F., TODD, P., WOOD, J.C.S., JETT, J. H.: Laser flow cytometric light scatter and fluorescence pulse width and pulse rise-time sizing of mammalian cells. J. Histochem. Cytochem. 27, 315, 1979 PubMedCrossRefGoogle Scholar
  23. 23.
    Mansberg, H. P., Saunders, A. M., Groner, W.: The Hemalog D white cell differential system. J. Histochem. Cytochem. 22, 711, 1974 PubMedCrossRefGoogle Scholar
  24. 24.
    MULLANEY, P. F., VAN DILLA, M. A., COULTER, J. R., DEAN, P. N.: Cell sizing: A light scattering photometer for rapid volume determination. Rev. Sci. Instrum. 40, 1029, 1969 PubMedCrossRefGoogle Scholar
  25. 25.
    MULLANEY, P. F., CROWELL, J. M., SALZMAN, G. C., MARTIN, J. C., HANSEN, K.M., GOOD, C.A., INGRAM, M.: Multiangle light scattering method for mammalian cell detection and identification. In: Pulse Cytophotometry. European Press, 1976, pp. 15–26Google Scholar
  26. 26.
    SALZMAN, G. C., CROWELL, J. M., HANSEN, K. M., INGRAM, M., MULLANEY, P.F.: Gynecology specimen analysis by multiangle light scattering in a flow system. J. Histochem. Cytochem. 24, 308, 1976 PubMedCrossRefGoogle Scholar
  27. 27.
    STEINKAMP, J. A., FULWYLER, M. J., COULTER, J. R., HIEBERT, R.D., Horney, J. L., Mullaney, P.F.: A new multiparameter separator for microscopic particles and biological cells. Rev. Sci. Instrum. 44, 1301, 1973 PubMedCrossRefGoogle Scholar
  28. 28.
    TOBEY, R. A., CRISSMAN, H. A. Comparative effects of three nitrosourea derivatives on mammalian cell cycle progression. Cancer Res. 35, 460, 1975 PubMedGoogle Scholar
  29. 29.
    TRAGANOS, F., GORSKI, A. J., DARZYNKIEWICZ, Z., SHARPLESS, T., MELAMED, M. A.: Rapid multiparameter analysis of cell stimulation in mixed lymphocytes culture reactions. J. Histochem. Cytochem. 25, 881, 1977 PubMedCrossRefGoogle Scholar
  30. 30.
    VAN DILLA, M. A., TRUJILLO, T. T., MULLANEY, P. F., COULTER, J. R.: Cell microfluorometry: Method for rapid fluorescence measurement. Science 163, 1213, 1969 PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • Mack J. Fulwyler
    • 1
  1. 1.Becton Dickinson FACS SystemsSunnyvaleUSA

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