Advertisement

Quantitative Analysis of the Chromatin of Lymphocytes: An Essay on Comparative Structuralism

  • Fernand Meyer
Conference paper

Abstract

With 26 letters we can form all the words we use, and with a few words it is possible to form an infinite number of different meaningful sentences. In our case, the letters will be a few simple neighborhood image transformations and area measurements. The paper shows how, by iterating these transformations, it is possible to obtain a good quantitative description of the nuclear structure of Feulgen-stained lymphocytes (CLL and normal). The fact that we restricted ourselves to a small number of image transformations made it possible to construct an image analysis system (TAS) able to do these transformations very quickly. We will see, successively, how to segment the nucleus itself, the chromatin, and the interchromatinic channels, how openings and closings lead to size and spatial distribution curves, and how skeletons may be used for measuring the lengths of interchromatinic channels.

Key Words

Mathematical morphology Segmentation Size distribution Skeletons Quantitative cytology Nuclear texture. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    BESSIS, M., MOHANDAS, N.: A diffractometric method for the measurement of cellular deformability. Blood Cells 1, 307, 1975 Google Scholar
  2. 2.
    BESSIS, M., MOHANDAS, N.: Deformability of normal shape altered and pathological red cells. Blood Cells 1, 315, 1975 Google Scholar
  3. 3.
    BESSIS, M., MOHANDAS, N., FEO, C.: Automated ektacytometry: A new method of measuring red cell quality and red cell indices. Blood Cells (in press) 1980 Google Scholar
  4. 4.
    BEUCHER, S., LANTUEJOUL, C.: Use of watersheds in contour detection. In: Proceedings of International Workshop on Image Processing: Real time edge and motion detection/estimation, Rennes, France (in press) 1980 Google Scholar
  5. 5.
    BEUCHER, S., LANTUEJOUL, C.: On the change of space in image analysis. In: Proceedings of the Vlth International Congress of Stereology, Salzburg, Austria. Mikroskopie, Vienna (in press) 1980 Google Scholar
  6. 6.
    BINET, J. L.: La Cytologie Quantitative. PUF, Paris, 1977 Google Scholar
  7. 7.
    BINET, J. L., LEPORRIER, M., DIGHIERO, G., CHARRON, D’ATHIS, P., VAUGIER, G., MERLE-BERAL, M., NATALI, J. C., NIZET, M. G., RAPHAEL, M., FOLLEZOU, J.-Y.: A clinical staging system for chronic lymphocytic leukemia. Cancer 855, 1977 Google Scholar
  8. 8.
    BROUET, J. C., SELIGMANN, M.: Chronic lymphocytic leukemia as an immunoproliferation disorder. Clin. Hematol. 6, 169–177Google Scholar
  9. 9.
    DIGHIERO, G., BINET, J. L., FOLLEZOU, J. Y., ROISIN, J. P., TERNYNCK, T.: Comparison of normal and CLL lymphocytes. Surface Ig determinants using peroxydase labelled antibodies. Blood 48, 559, 1975 Google Scholar
  10. 10.
    DUDA, R. O., HART, E.: Pattern Classification and Scene Analysis. New York, John Wiley and Sons, 1973 Google Scholar
  11. 11.
    DURIE, B. G., VAUHT, L., CHEN, Y. P., OLSON, G. B., SALMONS, S. E., BARTELS, P. H.: Discrimination between human T and B lymphocytes and monocytes by computer analysis of digitized data from scanning microphotometry. Blood 51, 579, 1978 PubMedGoogle Scholar
  12. 12.
    KLEIN, J. C., Serra, J.: The texture analyser. J. Microsc. 95, 349–356, 1972 CrossRefGoogle Scholar
  13. 13.
    KLEIN, J. C.: Conception et réalisation d’zune unité logique pour l’analyse quantitative d’images, Thèse de Docteur-Ingénieur, Nancy, 1976 Google Scholar
  14. 14.
    MATHERON, G.: Eléments pour une Théorie des Milieux Poreux. Paris, Masson, 1967 Google Scholar
  15. 15.
    MATHERON, G.: Random Sets and Integral Geometry. New York-London-Sydney-Toronto, John Wiley and Sons, 1975 Google Scholar
  16. 16.
    MEYER, F.: Contrast Features Extraction, Special Issues of Practical Metallography, Vol. 8. Stuttgart, Riederer, 1977 Google Scholar
  17. 17.
    MEYER, F.: Mathematical Morphology Used for Quantitative Cytology, Advanced Technobiology. Ed. by B. Rybac, Aalphen aan den Sijthoff & Noordhoff Rijn, The Netherlands, 1979 Google Scholar
  18. 18.
    MEYER, F.: Cytologie quantitative et morphologie mathématique. Thèse, Ecole des Mines, Paris 1979 Google Scholar
  19. 19.
    SERRA, J.: Image Analysis by Mathematical Morphology, New York, Academic (in press) 1980 Google Scholar
  20. 20.
    RAI, K. R., SAVILSKY, A., CRONKITE, E. P., CHANANA, A. D., LEVY, R., PASTERNACK, B.: Clinical staging of chronic lymphocytic leukemia. Blood 46, 2, 1975 Google Scholar
  21. 21.
    TANKE, H., VAN INGEN, E. M., PLOEM, J. S.: Acriflavine Feulgen Stilbene staining: A procedure for automated cervical cytology with a television based system (LEYTAS). Histochem. Cytochem. 27, 84, 1979 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • Fernand Meyer
    • 1
  1. 1.Centre de Géostatistique et de Morphologie Mathématique, Ecole des Mines de ParisFontainebleauFrance

Personalised recommendations