Quantitative Analysis of the Chromatin of Lymphocytes: An Essay on Comparative Structuralism

  • Fernand Meyer
Conference paper


With 26 letters we can form all the words we use, and with a few words it is possible to form an infinite number of different meaningful sentences. In our case, the letters will be a few simple neighborhood image transformations and area measurements. The paper shows how, by iterating these transformations, it is possible to obtain a good quantitative description of the nuclear structure of Feulgen-stained lymphocytes (CLL and normal). The fact that we restricted ourselves to a small number of image transformations made it possible to construct an image analysis system (TAS) able to do these transformations very quickly. We will see, successively, how to segment the nucleus itself, the chromatin, and the interchromatinic channels, how openings and closings lead to size and spatial distribution curves, and how skeletons may be used for measuring the lengths of interchromatinic channels.

Key Words

Mathematical morphology Segmentation Size distribution Skeletons Quantitative cytology Nuclear texture. 


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Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • Fernand Meyer
    • 1
  1. 1.Centre de Géostatistique et de Morphologie Mathématique, Ecole des Mines de ParisFontainebleauFrance

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