Objective Measures of Information From Blood Cells

  • Louis A. Kamentsky
Conference paper


An answer to the question, “The blood cell: what to measure? why?” is considered by proposing the use of indices derived from flow cytometric measurements. Indices derived from laser light scatter and fluorescence measurements of red cells, platelets, reticulocytes and leukocytes are described. To answer the “why” part of the question, I have proposed a statistical measurement evaluation technique based on information theory. This is illustrated by an example using indices to predict patient infection.

Key Words

Flow cytometry Red cells Platelets Reticulocytes Leukocytes Fluorescence Light scatter Information theory. 


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  1. 1.
    ADAMS, L. R., KAMENTSKY, L. A.: Machine characterization of human leukocytes by acridine orange fluorescence. Acta Cytol. 15, 289–291, 1971 PubMedGoogle Scholar
  2. 2.
    ADAMS, L. R., KAMENTSKY, L. A.: Fluorometric characterization of six classes of human leukocytes. Acta Cytol. 18, 389–391, 1974 PubMedGoogle Scholar
  3. 3.
    ADAMS, L. R., BOURGEOIS, D., KAMENTSKY, L. A.: Cytofluorographic differential leukocyte counts. Flow Cytometry and Sorting, MELAMED, M. R., MULLANEY, P. F., MENDEL, M. L., Jr., eds. New York, John Wiley & Sons, 1979, pp. 547–555Google Scholar
  4. 4.
    BRECHER, G., JAKOBEK, E. F., SCHNEIDERMAN, M. A., WILLIAMS, G. Z., SCHMIDT, P. J.: Size distribution of erythrocytes. Ann. NY Acad. Sci. 99, 242–261, 1962 PubMedCrossRefGoogle Scholar
  5. 5.
    CLARKSON, D. R., MOORE, E. M.: Reticulocyte size in nutritional anemias. Blood 48, 669–677, 1976 PubMedGoogle Scholar
  6. 6.
    COULTER, W. H.: High speed automatic blood cell counter and cell size analyzer. Proc. National Electronics Conference, 12, 1034–1040, 1956 Google Scholar
  7. 7.
    GILMER, P. R., JR., KOEPKE, J. A.: The reticulocyte: An approach to definition. Am. J. Clin. Pathol. 66, 262–267, 1976 PubMedGoogle Scholar
  8. 8.
    KAMENTSKY, L. A.: Cytology automation. Adv. Biol. Med. Phys. 14, 93–161, 1973 PubMedCrossRefGoogle Scholar
  9. 9.
    KAMENTSKY, L. A., LIU, C. N.: Computer-automated design of multifont print recognition logic. IBM J. Res. Dev. 7, 1–13, 1963 CrossRefGoogle Scholar
  10. 10.
    KAMENTSKY, L. A., THORELL, B.: Cell population identification studies. Acta Cytol. 14, 307–312, 1970 PubMedGoogle Scholar
  11. 11.
    LEWIS, P. M.: The characteristic selection problem in recognition systems. IRE Trans. Inf. Theory 8, 171–178, 1962 CrossRefGoogle Scholar
  12. 12.
    MANSBERG, H. P.: Optical techniques of particle counting. Technicon Congress, 1969. Advances in Automated Anal., Vol. I. White Plains, New York, Mediad, Inc., 1970, pp.213–220Google Scholar
  13. 13.
    MELAMED, M. R., ADAMS, L. R., ZIMRING, A., MURNICK, J. G., MAYER, K.: Preliminary evaluation of acridine orange as a vital stain for automated differential leukocyte counts. Am. J. Clin. Pathol. 57, 95–102, 1972 PubMedGoogle Scholar
  14. 14.
    MELAMED, M. R., ADAMS, L. R., TRAGANOS, F., ZIMRING, A., KAMENTSKY, L. A.: Acridine orange metachromasia for characterization of leukocytes in leukemia, lymphoma and other neoplasms. Cancer 29, 1361–1468, 1972 PubMedCrossRefGoogle Scholar
  15. 15.
    MELAMED, M. R., ADAMS, L. R., TRAGANOS, F., KAMENTSKY, L. A.: Blood granulocyte staining with acridine orange: Changes with infection. J. Histochem. Cytochem. 22, 526–530, 1974 PubMedCrossRefGoogle Scholar
  16. 16.
    MELAMED, M. R., KAMENTSKY, L. A.: Automated cytology. IN: International Review of Experimental Pathology, Vol. 14. New York, Academic Press, 1975, pp. 205–295Google Scholar
  17. 17.
    MELAMED, M. R., MULLANEY, P. F., MENDELSOHN, M. L.: Flow Cytometry and Sorting. New York, John Wiley & Sons, 1979 Google Scholar
  18. 18.
    SHANNON, C. E.: The Mathematical Theory of Communication. Urbana, University of Illinois Press, 1949 Google Scholar
  19. 19.
    VON BEHRENS, W. E.: Evidence of phylogenetic canalisation of the circulating platelet mass in man. Thromb. Haemostas. 27, 159–172, 1972 Google Scholar
  20. 20.
    WOODWARD, P. M.: Probability and Information Theory With Applications to Radar. London, Pergamon Press, 1953 Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • Louis A. Kamentsky
    • 1
  1. 1.Research and Development DepartmentOrtho InstrumentsWestwoodUSA

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