Two New Applications of the Immunoperoxidase Method: Cell-by-Cell Quantitation of Surface Immunoglobulins and Automated Recognition of B-Lymphocytes
Maturation of mice B-lymphocytes during ontogeny is accompanied by the appearance of a population containing intermediate to high amounts of SIg.
The CLL B-lymphocyte pattern as compared with that of normal B-lympho-cytes shows a decrease of SIg and a homogeneous distribution.
In the second application, mice splenic lymphocytes are incubated with anti-mouse Ig coupled with peroxidase and analyzed through Hemalog D. B-lymphocytes are separated from T-lymphocytes in a distinct population since they become positive for peroxidase.
Key WordsImmunocytochemistry Automation B-lymphocyte Chronic lymphocytic leukemia Ontogeny.
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