Cell Density Profile as a Measure of Erythrocyte Hydration: Therapeutic Alteration of Salt and Water Content in Normal and SS Red Blood Cells

  • Eugene P. Orringer
  • Marjorie E. S. Roer
  • John C. Parker
Conference paper


The incubation of red blood cells in high concentrations of sodium bicarbonate produces a net influx of salt and water resulting in the dilution of cell hemoglobin. After reinfusion into the donor, cells swollen in this manner can be readily identified in peripheral blood samples by their low density on phthalate gradients. It is proposed that this manipulation of cell water content may have therapeutic implications for sickle cell disease, since the rate of deoxyhemoglobin S gelation is retarded by small reductions in hemoglobin concentration.

Key Words

Anemia Sickle cell Cell volume regulation Erythrocyte density Erythrocyte membrane permeability. 


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Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • Eugene P. Orringer
    • 1
  • Marjorie E. S. Roer
    • 1
  • John C. Parker
    • 1
  1. 1.Division of Hematology, Departments of Medicine and Laboratory MedicineSchool of Medicine, The University of North CarolinaChapel HillUSA

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