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Ektacytometry and the Measurement of Cell Physical Properties. Commentary of papers

  • M. Bessis
  • N. Mohandas
  • C. Feo
  • N. Mohandas
  • M. R. Clark
  • M. S. Jacobs
  • W. Groner
  • S. B. Shohet
  • Howard C. Mel
Conference paper

Abstract

This commentary is organized in three parts: first, some remarks on cell size and number and population heterogeneity; second, a discussion of deformability and the factors contributing to it, and a comparison with two other methods of measuring it; and third, an analysis of the limitations of the “deformability index” as used by Bessis et al., along with suggestions for two simple transformations which convert their same experimental data into quantitative measures more closely related to deformability. The comments are principally directed toward the Bessis, Mohandas, Feo paper and to the Bull commentary. By emphasizing certain speculative possibilities for the ektacytometer, beyond its presently established capabilities, the authors, in effect, invite a more critical and focused response, made in an effort to redress an imbalance as viewed from present perspectives. We must impatiently await the future to assess how much of their speculations are prophetic and how much are wishful thinking (I suspect there will be some of both).

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References

  1. 1.
    MEL, H.C., YEE, J.P.: erythrocyte size and deformability studies by resistive pulse spectroscopy. Blood cells 1, 391–399, 1975 Google Scholar
  2. 2.
    MEL, H.C., MOHANDAS, N.: size deformability and form of red blood cells in relation to their hydration properties. colloque international du cnrs, n° 246:l‘eau et les systemes biologiques, ALFSEN, A., ED., CNRS, Paris, 311–316, 1976 Google Scholar
  3. 3.
    MEL, H.C., JUNG, D.G.: Isotonicity and red cell-membrane deformability. Biophys. J. 21, 119A, 1978Google Scholar
  4. 4.
    YEE, J.P., MEL, H.C.: Cell membrane and rheological mechanisms: Dynamic osmotic hemolysis of human erythrocytes and repair of ghosts as studied by resistive pulse spectroscopy. Biorheology 15, 321–339, 1978 PubMedGoogle Scholar
  5. 5.
    YEE, J.P.: Development and application of “resistive pulse spectroscopy”, studies on the size, form and deformability of red blood cells. P Thesis, University of California, Berkeley, California 94720, 1979. (Also U.S. Department of Energy, Lawrence Berkeley Laboratory, LBL-8640, January 1979)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • M. Bessis
    • 1
  • N. Mohandas
    • 1
  • C. Feo
    • 1
  • N. Mohandas
    • 1
  • M. R. Clark
    • 1
  • M. S. Jacobs
    • 1
  • W. Groner
    • 1
  • S. B. Shohet
    • 1
  • Howard C. Mel
    • 1
  1. 1.Institut de Pathologie Cellulaire Hôpital de BicêtreLe Kremlin-BicêtreFrance

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