Advertisement

Computer Analysis of Platelet Volumes

  • G. Dighiero
  • C. Lesty
  • M. Leporrier
  • M. C. Couty
Conference paper

Abstract

A Coulter ZB was used to measure electronically the size of circulating platelets collected from normal subjects. EDTA or ACD were used for collection. Three mathematical models of volume histograms were tested. The lognormal law model is clearly unacceptable if all particles are used, but is applicable in the particle range of 3–15 µ3. The model of a mixture of two lognormal populations closely approximates observed volume distribution. The model of three lognormal populations also approximates well when the largest volume population is divided into two subpopulations. The contribution of these results to an understanding of heterogeneity in platelet size is discussed.

Key Words

Automation Volumes Platelets 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    AMOROSI, E., GARG, S.K., KARPATKIN, S.: Heterogeneity of human platelets. IV. Identification of a young platelet population with Se75 selenomethionine. Br. J. Haematol. 21, 227–234, 1971 CrossRefGoogle Scholar
  2. 2.
    KARPATKIN, S.: Heterogeneity of human platelets. VI. Correlation of platelet function with platelet volume. Blood 51, 307–315, 1978 PubMedGoogle Scholar
  3. 3.
    MACDONALD, P.D.M.: Estimation of finite distribution mixtures. In: GUPTA, R.P., ed., Applied Statistics. North-Holland 1975, pp. 231–245Google Scholar
  4. 4.
    PAULUS, J.M., BURG, J., GROSDENT, J.C.: Control of platelet territory development in megakaryocytes. Blood Cells 5, 59–88, 1979 PubMedGoogle Scholar
  5. 5.
    PENNINGTON, D.G., STREAT FIELD, K., ROXBURGH, A.E.: Megakaryocytes and heterogeneity of circulating platelets. Br. J. Haematol. 34, 639–653, 1976 CrossRefGoogle Scholar
  6. 6.
    VALET, G., HOFMAN, H., RUHENSTROTH, BAUER, G.: The computer analysis of volume distribution curves. J. Histochem. Cytochem. 24, 231, 1976 PubMedCrossRefGoogle Scholar
  7. 7.
    YOUNG, IAN T.: Proof without prejudice: Use of the Kolmogorov-Smirnov test for the analysis of histograms from flow systems and other sources. J. Histochem. Cytochem. 25, 935–941, 1977 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • G. Dighiero
  • C. Lesty
  • M. Leporrier
  • M. C. Couty

There are no affiliations available

Personalised recommendations