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Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems

  • Tian-fei YuEmail author
  • Shu-li Shao
  • Xing-jun Xu
  • Jian-wei Lv
  • Ming Li
Chapter
  • 154 Downloads
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 134)

Abstract

In order to illuminate the antigenicity of porcine transmissible gastroenteritis virus (TGEV) spike protein B and C antigen sites, the truncated spike gene including B and C antigen sites of Chinese isolate TH-98 was expressed respectively in E.coli, baculovirus and pichia pastoris expression systems. Dot enzyme-linked immunosorbent assays (Dot-ELISA) based on these three recombinant proteins were developed preliminarily. Ten sera obtained correspondingly from ten piglets two months old which showed up clinical symptom were used for examination. The study indicates that the assays are rapid, reliable and sensitive and it has the potential for use as serological methods for TGEV diagnosis.

Keywords

Transmissible gastroenteritis virus Spike protein Expression Dot-ELISA 

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Copyright information

© Springer-Verlag GmbH Berlin Heidelberg 2012

Authors and Affiliations

  • Tian-fei Yu
    • 1
    Email author
  • Shu-li Shao
    • 1
  • Xing-jun Xu
    • 1
  • Jian-wei Lv
    • 1
  • Ming Li
    • 1
  1. 1.College of Life Science and Agriculture ForestryUniversity of QiqiharQiqiharChina

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