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Stable Isotopes of Carbon and Nitrogen as Tracers for Paleo-Diet Reconstruction

  • H. P. SchwarczEmail author
  • M. J. Schoeninger
Chapter
Part of the Advances in Isotope Geochemistry book series (ADISOTOPE)

Abstract

The isotopic compositions of the tissues of animals and humans are determined by the proportions of the various nutrients which they consume. This allows us to determine how much of each of their available foods were actually consumed over various parts of their lifetime. We apply this to ancient humans by analysis of bones, teeth and, rarely desiccated softer tissues (hair, skin). We review variations in δ13C and δ15N of known classes of nutrients (plants and animals), and discuss the chemical species whose isotopic composition can be analysed: collagen, bone-mineral, and lipids (cholesterol, etc.). Some variation in δ15N also arises from variations in δ15N of soil, particulate matter in the sea, and effect of aridity on N balance in terrestrial animals. Examples are presented of variation in δ13C due to consumption of maize (a C4 plant) and in δ15N due to trophic level effects in prehistoric hunter-gatherers.

Keywords

Isotopic Composition Dissolve Inorganic Carbon Crassulacean Acid Metabolism Tooth Enamel Bone Collagen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Aiello LC, Wheeler P (1995) The expensive-tissue hypothesis: the brain and the digestive system in human and primate evolution. Curr Anthrop 36:199–221Google Scholar
  2. Ambrose SH (1990) Preparation and characterization of bone and tooth collagen for isotopic analysis. J Archaeol Sci 17:431–451Google Scholar
  3. Ambrose S, Katzenberg MA (eds) (2000) Biogeochemical approaches to paleodietary analysis. Kluwer Academic, New York, p 269Google Scholar
  4. Ambrose SH, Norr L (1993) Experimental evidence for the relation of the carbon isotope ratios of whole diet and dietary protein to those of collagen and carbonate. In: Lambert JB, Grupe G (eds) Prehistoric human bone-archaeology at the molecular level. Springer, Berlin, pp 1–37Google Scholar
  5. Balasse M, Ambrose SH, Smith AB, Price TD (2002) The seasonal mobility model for prehistoric herders in the south-western Cape of South Africa assessed by isotopic analysis of sheep tooth enamel. J Archaeol Sci 29:917–932Google Scholar
  6. Barrett JH, Richards MP (2004) Evidence for marine resource intensification in identity, gender, religion and economy: new isotope and radiocarbon evidence in early historic Orkney, Scotland UK. Eur J Archaeol 7:249Google Scholar
  7. Bilezikian, John P. Raisz, Lawrence G Martin T. John (eds) (2008) Principles of bone biology. 3rd ed. Elsevier, BostonGoogle Scholar
  8. Bocherens H, Drucker DG, Billiou D, Patou-Mathis M, Vandermeersch B (2005) Isotopic evidence for diet and subsistence pattern of the Saint-Césaire I Neanderthal: review and use of a multi-source mixing model. J Hum Evol 49:71–87Google Scholar
  9. Cannon A, Schwarcz HP, Knyf M (1999) Marine-based subsistence trends and the stable-isotope analysis of dog bones from Namu, British Columbia. J Archaeol Sci 26:399–408Google Scholar
  10. Cerling TE, Harris J, Passey B (2003) Diets of East African bovidae based on stable isotope analyses. J Mammal 84:456–470Google Scholar
  11. Chisholm BS, Nelson DE, Schwarcz HP (1982) Stable carbon isotope ratios as a measure of marine versus terrestrial protein in ancient diets. Science 216:1131–1132Google Scholar
  12. Corr LT, Sealy JC, Horton MC, Evershed RP (2005) A novel marine dietary indicator utilizing compound-specific bone collagen amino acid δ13C values of ancient humans. J Archaeol Sci 32:321–330Google Scholar
  13. Corr LT, Richards MP, Jim S, Ambrose SH, Mackie A, Beattie O, Evershed RP (2008) Probing dietary change of the Kwaday Dan Ts’inchi individual, an ancient glacier body from British Columbia: I. Complementary use of marine lipid biomarker and carbon isotope signatures as novel indicators of a marine diet. J Archaeol Sci 35:2102–2110Google Scholar
  14. DeNiro MJ, Epstein S (1978) Influence of diet on the distribution of carbon isotopes in animals. Geochim Cosmochim Acta 42:495–506Google Scholar
  15. DeNiro MJ, Epstein S (1981) Influence of diet on the distribution of nitrogen isotopes in animals. Geochim Cosmochim Acta 45:341–351Google Scholar
  16. DeNiro MJ, Weiner S (1988) Organic matter within crystalline aggregates of hydroxyapatite: A new substrate for stable isotopic and possibly other biochemical analyses of bone. Geochim. Cosmochim Acta 52:2415–2423Google Scholar
  17. DeNiro MJ (1985) Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nature 317:806–809Google Scholar
  18. DeNiro MJ, Schoeninger MJ, Hastorf CA (1985) Effect of heating on the stable carbon and nitrogen isotope ratios of bone collagen. J Archaeol Sci 12:1–7Google Scholar
  19. Dudd SN, Evershed RP (1998) Direct demonstration of milk as an element of archaeological economies. Science 282:1478–1481Google Scholar
  20. Evans RD (2007) Soil nitrogen isotope composition. In: Mitchener R, Lajtha, K (eds) Stable Isotopes in Ecology and Environmental Science. 2nd Ed. Blackwell, Oxford, 83–98Google Scholar
  21. Evans RD (2008) Soil nitrogen isotope composition. In: Michener RH, Lajtha K (eds) Stable isotopes in ecology and environmental science, 2nd edn. Wiley, Chichester, pp 83–98Google Scholar
  22. Evershed RP (2008) Organic residue analysis in archaeology: the archaeological biomarker revolution. Archaeometry 50:895–924Google Scholar
  23. Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40:503–537Google Scholar
  24. Fogel ML, Tuross N, Johnson BJ, Miller GH (1997) Biogeochemical record of ancient humans. Org Geochem 27:275–287Google Scholar
  25. Fogel ML, Tuross N (2003) Extending the limits of paleodietary studies of humans with compound specific carbon isotope analysis of amino acids. J Archaeol Sci 30:535–545Google Scholar
  26. Fry B, Jeng WL, Scalan RS, Parker PL, Baccus J (1978) δ13C food web analysis of a Texas sand community. Geochim Cosmochim Acta 42:1299–1302Google Scholar
  27. Fry B (2006) Stable isotope ecology. Springer, New YorkGoogle Scholar
  28. Fuller BT, Richards MP, Mays SA (2003) Stable carbon and nitrogen isotope variation in tooth dentine serial sectionals from Wharram Percy. J Archaeol Sci 30:1673–1684Google Scholar
  29. Guo L, Tanaka T, Wang D, Tanaka N, Murata A (2004) Distributions, speciation and stable isotope composition of organic matter in the southeastern Bering Sea. Mar Chem 91:211–226Google Scholar
  30. Harrison RG, Katzenberg MA (2003) Paleodiet studies using stable carbon isotopes from bone apatite and collagen: examples from Southern Ontario and San Nicolas Island, California. J Anthropol Arch 22:227–244Google Scholar
  31. Hastorf CA, DeNiro MJ (1985) Reconstruction of prehistoric plant production and cooking practices by a new isotopic method. Nature 315:489–491Google Scholar
  32. Heaton JHE, Vogel JC, von la Chevallerie G, Collet G (1986) Climatic influence on the isotopic composition of bone nitrogen. Nature 322:822–823Google Scholar
  33. Hedges REH, van Klinken G (2000) “Consider a spherical cow ⋯” – on modeling and diet. In: Ambrose S, Katzenberg MA (eds) Biogeochemical approaches to paleodietary analysis. Kluwer Academic, New York, pp 211–241Google Scholar
  34. Hedges REM, Reynard L (2007) Nitrogen isotopes and the trophic level of humans in archaeology. J Archaeol Sci 34:1240–1251Google Scholar
  35. Hedges REM, Clement JG, Thomas CDL, O’Connell TC (2007) Collagen turnover in the adult femoral mid-shaft: modeled from anthropogenic radiocarbon tracer measurements. Am J Phys Anthropol 133(2):808–816Google Scholar
  36. Howland MR, Corr LT, Young SM, Jones V, Jim S, van der Merwe NJ, Mitchell AD, Evershed RP (2003) Expression of the dietary isotope signal in the compound-specific δ13C values of pig bone lipids and amino acids. Int J Osteoarch 13:54–65Google Scholar
  37. Hu Y, Shang H, Tong H, Nehlich O, Liu W, Zhao C, Yu J, Wang C, Trinkaus E, Richards M (2009) Stable isotope dietary analysis of the Tianyuan 1 early modern human. Proc Natl Acad Sci (USA) 106:10971–10974Google Scholar
  38. Jim S, Ambrose SH, Evershed RP (2004) Stable carbon isotopic evidence for differences in the dietary origin of bone cholesterol, collagen and apatite: implications for their use in palaeodietary reconstruction. Geochim Cosmochim Acta 68:61–72Google Scholar
  39. Katzenberg MS (2000) Stable isotope analysis: a tool for studying past diet, demography and life history. In: Katzenberg MA, Saunders SR (eds) The biological anthropology of the human skeleton. Wiley, New York, pp 305–327Google Scholar
  40. Katzenberg MA, Schwarcz HP, Knyf M, Melbye FJ (1995) Stable isotope evidence for maize horticulture and paleodiet in southern Ontario, Canada. Am Antiquity 60:335–350Google Scholar
  41. Katzenberg MA, Herring DA, Saunders SR (1996) Weaning and infant mortality: evaluating the skeletal evidence. Yrbk Phys Anthropol 39:177–199Google Scholar
  42. Keenleyside A, Schwarcz HP, Stirling L, Lazreg NB (2008) Stable isotopic evidence for diet in a Roman and late Roman population from Leptiminus, Tunisia. J Archaeol Sci 36:51–63Google Scholar
  43. Kellner CM, Schoeninger MJ (2007) A simple carbon isotope model for reconstructing human diet. Am J Phys Anthropol 133:1112–1127Google Scholar
  44. Kellner CM, Schoeninger M, Spielmann KA, Moore K (2010) Stable isotope data show temporal stability in diet at Pecos Pueblo and diet variation among southwest Pueblos. In: Morgan ME (ed) Pecos revisited. Harvard University Press, Cambridge, pp 79–91Google Scholar
  45. Krueger HW, Sullivan CH (1984) Models for carbon isotope fractionation between diet and bone. In: Turnlund JE, Johnson PE (eds) Stable isotopes in nutrition. American Chemical Society Symposium Series 258. American Chemical Society, Washington, pp 205–222Google Scholar
  46. Lebon M, Reiche I, Bahain J-J, Chadefaux C, Moigne A-M, Fröhlich F, Sémah F, Schwarcz H, Falguères C (2010) New parameters for the characterization of diagenetic alterations and heat-induced changes of fossil bone mineral using Fourier Transform Infrared Spectrometry. J Archaeol Sci 37:2265–2276Google Scholar
  47. Lee-Thorp JA, Sealy JC, van der Merwe NJ (1989) Stable carbon isotope ratio differences between bone collagen and bone apatite, and their relationship to diet. J Archaeol Sci 16:585–599Google Scholar
  48. Lee-Thorp JA, van der Merwe NJ, Brain CK (1994) Diet of Australopithecus robustus at Swartkrans from stable carbon isotopic analysis. J Hum Evol 27:361Google Scholar
  49. Little EA, Schoeninger MJ (1995) The late Woodland diet on Nantucket Island and the problem of maize in coastal New England. Am Antiquity 60:351–368Google Scholar
  50. Manolagas S (2000) Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr Rev 21:115–137Google Scholar
  51. Marino BD, McElroy JB (1991) Isotopic composition of atmospheric CO2 inferred from carbon in C4 plant cellulose. Nature 349:127–131Google Scholar
  52. Masters PM (1987) Preferential preservation of noncollagenous protein during bone diagenesis: implications for chronometric and stable isotopic measurements. Geochim Cosmochim Acta 51:3209–3214Google Scholar
  53. Minagawa M, Wada E (1984) Stepwise enrichment of 15N along food chains: further evidences and the relation between δ15N and animal age. Geochim Cosmochim Acta 48:1135–1140Google Scholar
  54. Miracle P, Milner N (2008) Consuming Passions and Patterns of Consumption. [electronic resource] Cambridge UK, McDonald for Archaeological Research, Institute, Ebooks CorporationGoogle Scholar
  55. Moore JW, Semmens BX (2008) Incorporating uncertainty and prior information into stable isotope mixing models. Ecol Lett 11:470–480Google Scholar
  56. Morton J, Schwarcz HP (2004) Paleodietary implications from isotopic analysis of food residues on prehistoric Ontario ceramics. J Archaeol Sci 31:503–517Google Scholar
  57. Murphy BP, Bowman DMJS (2009) The carbon and nitrogen isotope composition of Australian grasses in relation to climate. Funct Ecol 23:1040–1049Google Scholar
  58. Murray M, Schoeninger MJ (1988) Diet, status, and complex social structure in Iron Age Central Europe: some contributions of bone chemistry. In: Gibson B, Geselowitz M (eds) Tribe and polity in late prehistoric Europe. Plenum Press, New York, pp 157–178Google Scholar
  59. Naito YI, Honch NV, Chikaraishi Y, Ohkouchi N, Yoneda M (2010) Quantitative evaluation of marine protein contribution in ancient diets based on nitrogen isotope ratios of individual amino acids in bone collagen: an investigation at the Kitakogane Jomon site. Am J Phys Anth 143:31–40Google Scholar
  60. Newsome SD, Phillips DL, Culleton BJ, Guilderson TP, Koch PL (2004) Dietary reconstruction of an early to middle Holocene human population from the central California coast: insights from advanced stable isotope mixing models. J Archaeol Sci 31:1101–1115Google Scholar
  61. O’Connell TC, Hedges REM (1999) Isotopic composition of hair and bone: archaeological analyses. J Archaeol Sci 26:661–665Google Scholar
  62. Passey B, Robinson TF, Ayliffe LK, Cerling TE, Sponheimer MM, Dearing D, Roeder BL, Ehleringer JR (2005) Carbon isotope fractionation between diet, breath CO2, and bioapatite in different mammals. J Archaeol Sci 32:1459–1470Google Scholar
  63. Pechenkina EA, Ambrose SH, Xiaolin M, Benfer RA Jr (2005) Reconstructing northern Chinese Neolithic subsistence practices by isotopic analysis. J Archaeol Sci 32:1176–1189Google Scholar
  64. Peterson BJ, Fry B (1987) Stable isotopes in ecosystem studies. Ann Rev Ecol Syst 18:293–320Google Scholar
  65. Pfeiffer S, Sealy J (2006) Body size among Holocene foragers of the Cape Ecozone, southern Africa. Am J Phys Anth 129:1–11Google Scholar
  66. Phillips DL, Gregg JW (2003) Source partitioning using stable isotopes: coping with too many sources. Oecologia 136:261–269Google Scholar
  67. Prowse T, Schwarcz HP, Saunders S, Macchiarelli R, Bondioli L (2003) Isotopic paleodiet studies of skeletons from the Imperial Roman-age cemetery of Isola Sacra, Rome, Italy. J Archaeol Sci 31:259–272Google Scholar
  68. Richards MP (2009) Stable isotope evidence for European Upper Paleolithic human diets. In:Hublin J-J, Richards MP. The Evolution of Hominin Diets, Springer, Berlin, 251–258Google Scholar
  69. Richards MP, Trinkaus E (2009) Isotopic evidence for the diets of European Neanderthals and early modern humans. PNAS 106:16034–16039Google Scholar
  70. Richards MP, Schulting RJ, Hedges REM (2003) Sharp shift in diet at the onset of the Neolithic. Nature 425:366Google Scholar
  71. Richards MP, Pettitt PB, Trinkaus E, Smith FE, Paunovic M, Karavanic I (2000) Neanderthal diet at Vindija and Neanderthal predation: the evidence from stable isotopes. Proc Nat Acad Sci USA 97:7663–7666Google Scholar
  72. Sachs JP, Repeta DJ, Goericke R (1999) Nitrogen and carbon isotopic ratios of chlorophyll from marine phytoplankton. Geochim Cosmochim Acta 63:1431–1441Google Scholar
  73. Schoeninger MJ, DeNiro MJ (1984) Nitrogen and carbon stable isotope ratios of bone collagen reflect marine and terrestrial components of prehistoric human diet. Geochim Cosmochim Acta 48:625–639Google Scholar
  74. Schoeninger MJ, DeNiro MJ, Tauber H (1983) Stable nitrogen isotope ratios of bone collagen reflect marine and terrestrial components of prehistoric human diet. Science 220:1381–1383Google Scholar
  75. Schoeninger MJ, Moore K (1992) Bone stable isotope studies in archaeology. J World Prehistory 6:247–296Google Scholar
  76. Schoeninger MJ, Moore KM, Murray ML (1989) Detection of bone preservation in archaeological and fossil samples. J Appl Geochem 4:281–292Google Scholar
  77. Schoeninger MJ, Moore J, Sept JM (1999) Subsistence strategies of two ‘savanna’ chimpanzee populations: the stable isotope evidence. Am J Primatol 47:297–314Google Scholar
  78. Schoeninger MJ, van der Merwe NJ, Moore K, Lee-Thorp J, Larsen CS (1990) Decrease in diet quality between the prehistoric and contact periods. In: Larsen CS (ed) The Archaeology of Mission Santa Catalina de Guale: 2. Biocultural Interpretations of a Population in Transition. Anthropological Papers of the American Museum of Natural History 68. pp 78–93, American Museum of Natural History, New YorkGoogle Scholar
  79. Schurr MR (1998) Using stable nitrogen isotopes to study weaning behavior in past populations. World Archaeol 30:327–342Google Scholar
  80. Schwarcz HP (1991) Some theoretical aspects of isotope paleodiet studies. J Archaeol Sci 18:261–275Google Scholar
  81. Schwarcz HP, Melbye J, Katzenberg MA, Knyf M (1985) Stable isotopes in human skeletons of southern Ontario: reconstructing paleodiet. J Archaeol Sci 12:187–206Google Scholar
  82. Schwarcz HP, Schoeninger M (1991) Stable isotope analyses in human nutritional ecology. Yearb Phys Anthropol 34:283–321Google Scholar
  83. Schwarcz HP, White CD (2004) The grasshopper or the ant? Cultigen-use strategies in ancient Nubia from C-13 analyses of human hair. J Archaeol Sci 31:753–762Google Scholar
  84. Schwarcz HP, Dupras T, Fairgrieve SI (1999) 15N enrichment in the Sahara: in search of a global relationship. J Archaeol Sci 26:629–636Google Scholar
  85. Semmens BX, Moore JW (2008) MixSIR (Version 1.0) http://conserver.iugo-cafe.org/node/870/download (accessed June 3 2011)
  86. Smith CI, Fuller BT, Choy K, Richards MP (2009) A three-phase liquid chromatographic method for δ13C analysis of amino acids from biological protein hydrolysates using liquid chromatography–isotope ratio mass spectrometry. Anal Biochem 390:165–172Google Scholar
  87. Spangenberg JE, Jacomet S, Schibler J (2006) Chemical analyses of organic residues in archaeological pottery from Arbon Bleiche 3, Switzerland – evidence for dairying in the late Neolithic. J Archaeol Sci 33:1–13Google Scholar
  88. Sponheimer M, Lee-Thorp JA (1999) Isotopic evidence for the diet of an early hominid, Australopithecus africanus. Science 283:368–370Google Scholar
  89. Sponheimer M, Robinson TF, Roeder BL, Passey BH, Ayliffe LK, Cerling TE, Dearing MD, Ehleringer JR (2003) An experimental study of nitrogen flux in llamas: is 14N preferentially excreted? J Archaeol Sci 30:1649–1655Google Scholar
  90. Stafford TW Jr, Brendel K, Duhamel RC (1988) Radiocarbon, 13C and 15N analysis of fossil bone: removal of humates with XAD-2 resin. Geochim Cosmochim Acta 52:2257–2267Google Scholar
  91. Stott AW, Evershed RP, Jim S, JonesV RJM, Tuross N, Ambrose S (1999) Cholesterol as a new source of palaeodietary information: experimental approaches and archaeological applications. J Archaeol Sci 26:705–16Google Scholar
  92. Steele KW, Daniel RMJ (1978) Fractionation of nitrogen isotopes by animals: a further complication to the use of variations in the natural abundance of 15N for tracer studies. J Agric Sci 90:7–9Google Scholar
  93. Stuart-Williams H, Schwarcz HP, White C, Spence M (1996) The isotopic composition and diagenesis of human bone at Teotihuacan and Oaxaca, Mexico. Paleogeogr Paleoclimatol Paleoecol 126:1–14Google Scholar
  94. Suess HE (1955) Radiocarbon concentration in modern wood. Science 122:415–417Google Scholar
  95. Tauber H (1981) 13C evidence for dietary habits of prehistoric man in Denmark. Nature 292:332–333Google Scholar
  96. Tieszen L, Fagre T (1993) Effect of diet quality and composition on the isotopic composition of respiratory CO2, bone collagen, bioapatite, and soft tissues. In: Lambert JB, Grupe G (eds) Prehistoric human bone: archaeology at the molecular level. Springer, BerlinGoogle Scholar
  97. Trueman CN, Privat K, Field J (2008) Why do crystallinity values fail to predict the extent of diagenetic alteration of bone mineral? Palaeogeog Palaeoclim Palaeoecol 266:160–167Google Scholar
  98. Ungar PS, Teaford M (2002) Human diet: its origin and evolution. Bergin & Garvey, Westport, p 206Google Scholar
  99. Urey HC (1947) The thermodynamic properties of isotopic substances. J Chem Soc 1947, 562–581Google Scholar
  100. U.S. Federal Register (2010) Department of the Interior, National Park Service, Native American Graves Protection and Repatriation Review Committee, Findings Related to the Identity of Cultural Items in the Possession of the American Museum of Natural History, New York, NY, Federal Register, Notices, Vol. 75, No. 67/Thursday, April 8, 2010Google Scholar
  101. van der Merwe NJ, Medina E (1991) The canopy effect, carbon isotope ratios and foodwebs in Amazonia. J Archaeol Sci 18:249–259Google Scholar
  102. van der Merwe NJ, Vogel JC (1978) 13C content of human collagen as a measure of prehistoric diet in woodland North America. Nature 276:815–816Google Scholar
  103. Vogel J, van der Merwe N (1977) Isotopic evidence for early maize cultivation in New York State. Am Antiquity 42:238–242Google Scholar
  104. Walker PL, DeNiro M (1986) Stable nitrogen and carbon isotope ratios in bone collagen as indices of prehistoric dietary dependence on marine and terrestrial resources in Southern California. Am J Phys Anthropol 71:51–61Google Scholar
  105. Wallace BP, Seminoff JA, Kilham SS, Spotila JR, Dutton PH (2006) Leatherback turtles as oceanographic indicators: stable isotope analyses reveal a trophic dichotomy between ocean basins. Mar Biol 149:953–960Google Scholar
  106. Warinner C, Tuross N (2009) Alkaline cooking and stable isotope tissue-diet spacing in swine: archaeological implications. J Archaeol Sci 36(8):1690–1697Google Scholar
  107. Wright LE, Schwarcz HP (1998) Stable carbon and oxygen isotopes in human tooth enamel: identifying breast-feeding in prehistory. Am J Phys Anthropol 106:1–18Google Scholar
  108. Yoneyama T, Ohta Y, Ohtani T (1983) Variations of natural δ13C and δ15N abundances in the rat tissues and their correlation. Radioisotopes 32:330–332Google Scholar

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Authors and Affiliations

  1. 1.School of Geography and Earth SciencesMcMaster UniversityHamiltonCanada
  2. 2.University of California at San DiegoLa JollaUSA

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