Nanobiotechnology: Soft Lithography

  • Elisa Mele
  • Dario Pisignano
Part of the Progress in Molecular and Subcellular Biology book series (PMSB, volume 47)


An entirely new scientific and technological area has been born from the combination of nanotechnology and biology: nanobiotechnology. Such a field is primed especially by the strong potential synergy enabled by the integration of technologies, protocols, and investigation methods, since, while biomolecules represent functional nanosystems interesting for nanotechnology, micro- and nano-devices can be very useful instruments for studying biological materials. In particular, the research of new approaches for manipulating matter and fabricating structures with micrometre- and sub-micrometre resolution has determined the development of soft lithography, a new set of non-photolithographic patterning techniques applied to the realization of selective proteins and cells attachment, microfluidic circuits for protein and DNA chips, and 3D scaffolds for tissue engineering. Today, soft lithographies have become an asset of nanobiotechnology. This Chapter examines the biological applications of various soft lithographic techniques, with particular attention to the main general features of soft lithography and of materials commonly employed with these methods. We present approaches particularly suitable for biological materials, such as microcontact printing (μCP) and microfluidic lithography, and some key micro- and nanobiotechnology applications, such as the patterning of protein and DNA microarrays and the realization of microfluidic-based analytical devices.


Soft Lithography Microcontact Printing Conformal Contact Microfluidic Network Soft Lithographic Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Elisa Mele
    • 1
  • Dario Pisignano
    • 1
  1. 1.National Nanotechnology Laboratory of Istituto Nazionale di Fisica della Materia-Consiglio Nazionale delle Ricerche, Distretto Tecnologico ISUFI, Università degli Studi di Leccevia ArnesanoItaly

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