Taxadiene synthase

Part of the Springer Handbook of Enzymes book series (HDBKENZYMES, volume S7)


Breast Cancer Reaction Type Cyclization Reaction Commit Step Farnesyl Diphosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Hezari, M.; Ketchum, R.E.B.; Gibson, D.M.; Croteau, R.: Taxol production and taxadiene synthase activity in Taxus canadensis cell suspension cultures. Arch. Biochem. Biophys., 337, 185–190 (1997)CrossRefPubMedGoogle Scholar
  2. [2]
    Wildung, M.R.; Croteau, R.: A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis. J. Biol. Chem., 271, 9201–9204 (1996)CrossRefPubMedGoogle Scholar
  3. [3]
    Koepp, A.E.; Hezari, M.; Zajicek, J.; Stofer Vogel, B.; LaFever, R.; Lewis, N.G.; Croteau, R.: Cyclization of geranylgeranyl diphosphateto taxa-4(5),-11(12)-diene is the comitted step of taxol biosynthesis in pacific yew. J. Biol. Chem., 270, 8686–8690 (1995)CrossRefPubMedGoogle Scholar
  4. [4]
    Lin, X.; Hezari, M.; Koepp, A.E.; Floss, H.G.; Croteau, R.: Mechanism of taxadiene synthase, a diterpene cyclase that catalyzes the first step of taxol biosynthesis in pacific yew. Biochemistry, 35, 2968–2977 (1996)CrossRefPubMedGoogle Scholar
  5. [5]
    Williams, D.C.; Carroll, B.J.; Jin, Q.; Rithner, C.D.; Lenger, S.R.; Floss, H.G.; Coates, R.M.; Williams, R.M.; Croteau, R.: Intramolecular proton transfer in the cyclization of geranylgeranyl diphosphate to the taxadiene precursor of taxol catalyzed by recombinant taxadiene synthase. Chem. Biol., 7, 969–977 (2000)CrossRefPubMedGoogle Scholar
  6. [6]
    Williams, D.C.; Wildung, M.R.; Jin, A.Q.; Dalal, D.; Oliver, J.S.; Coates, R.M.; Croteau, R.: Heterologous xpression and characterization of a “Pseudomature” form of taxadiene synthase involved in paclitaxel (taxol) biosynthesis and evaluation of a potential intermediate and inhibitors of the multistep diterpene cyclization reaction. Arch. Biochem. Biophys., 379, 137–146 (2000)CrossRefPubMedGoogle Scholar
  7. [7]
    Huang, Q.; Roessner, C.A.; Croteau, R.; Scott, A.I.: Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol. Bioorg. Med. Chem., 9, 2237–2242 (2001)CrossRefPubMedGoogle Scholar
  8. [8]
    Huang, Q.; Williams, H.J.; Roessner, C.A.; Scott, A.I.: Sesquiterpenes produced by truncated taxadiene synthase. Tetrahedron Lett., 41, 9701–9704 (2000)CrossRefGoogle Scholar
  9. [9]
    Jin, Y.; Williams, D.C.; Croteau, R.; Coates, R.M.: Taxadiene synthase-catalyzed cyclization of 6-fluorogeranylgeranyl diphosphate to 7-fluoroverticillenes. J. Am. Chem. Soc., 127, 7834–7842 (2005)CrossRefPubMedGoogle Scholar
  10. [10]
    Jin, Q.; Williams, D.C.; Hezari, M.; Croteau, R.; Coates, R.M.: Stereochemistry of the macrocyclization and elimination steps in taxadiene biosynthesis through deuterium labeling. J. Org. Chem., 70, 4667–4675 (2005)CrossRefPubMedGoogle Scholar
  11. [11]
    Chow, S.Y.; Williams, H.J.; Huang, Q.; Nanda, S.; Scott, A.I.: Studies on taxadiene synthase: interception of the cyclization cascade at the isocembrene stage with GGPP analogues. J. Org. Chem., 70, 9997–10003 (2005)CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Personalised recommendations