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Pleiomorphic viruses revealed by cryo tomography: the structure of coronaviruses

  • M. Bárcena
  • W. Baterlink
  • G. T. Oostergetel
  • A. Verklijk
  • P. J. M. Rottier
  • A. J. Koster
  • B. J. Bosch
Conference paper
  • 102 Downloads

Abstract

Cryo-electron microscopy has provided in the last decades a wealth of three-dimensional information on viral structures. However, most of this knowledge stems from single particle methods, which rely on averaging and are therefore restricted to viral homogenous structures such as icosahedral capsids [1]. This type of analysis excludes pleiomorphic viruses, that is, viruses that, having defined general architectures, arrange their components into different shapes or sizes. Cryo-electron tomography, which provides three-dimensional reconstructions of unique specimens, is now starting to shed light into the structure of this type of viruses [2].

Keywords

cryo-electron tomography coronavirus murine hepatitis virus 

References

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    Acknowledgments. This work was partially supported by a Marie Curie Intra-European fellowship to M.B. (MEIF-CT-2004-501540)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • M. Bárcena
    • 1
  • W. Baterlink
    • 2
  • G. T. Oostergetel
    • 3
  • A. Verklijk
    • 4
  • P. J. M. Rottier
    • 2
  • A. J. Koster
    • 1
  • B. J. Bosch
    • 2
  1. 1.EM Section, Molecular Cell BiologyLeiden University Medical CenterLeidenThe Netherlands
  2. 2.Virology Division, Infectious Diseases and Immunology, Faculty of VeterinaryUtrecht UniversityUtrechtThe Netherlands
  3. 3.Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology InstituteUniversity of GroningenGroningenThe Netherlands
  4. 4.Molecular Cell BiologyUtrecht UniversityUtrechtThe Netherlands

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