Ebolavirus and Other Filoviruses

  • E. Leroy
  • J. P. Gonzalez
  • X. Pourrut
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 315)

Since Ebola fever emerged in Central Africa in 1976, a number of studies have been undertaken to investigate its natural history and to characterize its transmission from a hypothetical reservoir host(s) to humans. This research has comprised investigations on a variety of animals and their characterization as intermediate, incidental, amplifying, reservoir, or vector hosts. A viral transmission chain was recently unveiled after a long absence of epidemic Ebola fever. Animal trapping missions were carried out in the Central African rain forest in an area where several epidemics and epizootics had occurred between 2001 and 2005. Among the various animals captured and analyzed, three species of fruit bats (suborder Megachiroptera) were found asymptomatically and naturally infected with Ebola virus: Hypsignathus monstrosus (hammer-headed fruit beats), Epomops franqueti (singing fruit bats), and Myonycteris torquata (little collared fruit bats).


Reservoir Host Nipah Virus Secondary Host Marburg Virus Incidental Host 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Allela L, Boury O, Pouillot R, Delicat A, Yaba P, Kumulungui B, Rouquet P, Gonzalez JP, Leroy EM (2005) Ebola virus antibody prevalence in dogs and human risk. Emerg Infect Dis 11:385–390.PubMedGoogle Scholar
  2. Amengual B, Whitby JE, King A, Cobo JS, Bourhy H (1997) Evolution of European bat lyssaviruses. J Gen Virol 78:2319–2328.PubMedGoogle Scholar
  3. Arata AA, Johnson B (1977) Approaches towards studies on potential reservoirs of viral haemorrhagic fever in southern Sudan. In: Pattyn SR (ed) Ebola virus haemorrhagic fever. Elsevier/Netherland biomedical, Amsterdam, pp 191–202.Google Scholar
  4. Badrane H, Tordo N (2001) Host switching in Lyssavirus history from the Chiroptera to the Carnivora orders. J Virol 75:8096–8104.CrossRefPubMedGoogle Scholar
  5. Bausch DG, Borchert M, Grein T, Roth C, Swanepoel R, Libande ML, Talarmin A, Bertherat E, Muyembe-Tamfum JJ, Tugume B, Colebunders R, Konde KM, Pirad P, Olinda LL, Rodier GR, Campbell P, Tomori O, Ksiazek TG, Rollin PE (2003) Risk factors for Marburg hemorrhagic fever Democratic Republic of the Congo. Emerg Infect Dis 9:1531–1537.PubMedGoogle Scholar
  6. Bergmans W (1989) Taxonomy and biogeography of African fruit bats (Mammalia Megachiroptera). Beaufortia 39:89–152.Google Scholar
  7. Bertherat E, Talarmin A, Zeller H (1999) La République Démocratique du Congo: Entre guerre civile et Virus de Marburg. Le Comité de Cooridantion Scienctifique et Technique International pour l’épideemie de Durba. Med Tropic 59:201–204.Google Scholar
  8. Breman JG, Johnson KM, van der Groen G, Robbins CB, Szczeniowski MV, Ruti K, Webb PA, Meier F, Heymann DL (1999) A search for Ebola virus in animals in the Democratic Republic of the Congo and Cameroon: ecologic, virologic, and serologic surveys, 1979–1980. J Infect Dis 179:S139–S147.CrossRefPubMedGoogle Scholar
  9. Calisher CH, Childs JE, Field HE, Holmes KV, Schountz T (2006) Bats: important reservoir hosts of emerging viruses. Clin Microbiol Rev 19:531–545.CrossRefPubMedGoogle Scholar
  10. Centers for Disease Control (1996) Ebola-Reston virus infection among quarantined nonhuman primates–Texas, 1996. Morb Mortal Wkly Rep 45:314–316.Google Scholar
  11. Constantine DG, Tierkel ES, Kleckner MD, Hawkins DM (1968) Rabies in New Mexico cavern bats. Pub Health Rep 83:303–316.Google Scholar
  12. Feldmann H, Wahl-Jensen V, Jones SM, Stroher U (2004) Ebola virus ecology: a continuing mystery. Trends Microbiol 12:433–437.CrossRefPubMedGoogle Scholar
  13. Formenty P, Boesch C, Wyers M, Steiner C, Donati F, Dind F, Walker F, Le Guenno B (1999) Ebola virus outbreak among wild chimpanzees living in a rain forest of Cote d’Ivoire. J Infect Dis 179 [Suppl 1]:S120–S126.CrossRefGoogle Scholar
  14. Geisbert TW, Jahrling PB, Hanes MA, Zack PM (1992) Association of Ebola-related Reston virus particles and antigen with tissue lesions of monkeys imported to the United States. J Comp Pathol 106:137–152.CrossRefPubMedGoogle Scholar
  15. Gonzalez JP, McCormick JB, Saluzzo JF, Georges AJ (1983) Les fièvres hémorragiques africaines d’origine virale en République Centrafricaine. Cah ORSTOM Ser Ent Méd Parasit XXI:119–130.Google Scholar
  16. Gonzalez JP, Herbreteau V, Morvan J, Leroy EM (2005) Ebola virus circulation in Africa: a balance between clinical expression and epidemiological silence. Bull Soc Pathol Exot 98:210–217.PubMedGoogle Scholar
  17. Halpin K, Young PL, Field HE, Mackenzie JS (2000) Isolation of Hendra virus from pteropid bats: a natural reservoir of Hendra virus. J Gen Virol 81:1927–1932.PubMedGoogle Scholar
  18. Heymann DL, Weisfeld JS, Webb PA, Johnson KM, Cairns T, Berquist H (1980) Ebola hemorrhagic fever: Tandala 1977–1978. J Infect Dis 142:372–376.PubMedGoogle Scholar
  19. Hill JE, Smith JD (1984) Bats: a natural history. University of Texas Press, Austin.Google Scholar
  20. Huijbregts B, De Wachter P, Ndong Obiang S, Akou Ella M (2003) Ebola and the decline of gorillaGorilla gorilla and chimpanzeePan troglodytes populations in Minkebe forest, north-eastern Gabon. Oryx 37:437–443.CrossRefGoogle Scholar
  21. Jahrling PB, Geisbert TW, Jaax NK, Hanes MA, Ksiazek TG, Peters CJ (1996) Experimental infection of cynomolgus macaques with Ebola-Reston filoviruses from the 1989–1990 US epizootic. Arch Virol Suppl 11:115–134.PubMedGoogle Scholar
  22. Johnson KM (1978) Ebola haemorrhagic fever in Zaire (1976) Bull World Health Organ 56:271–293.Google Scholar
  23. Kiley MP, Bowen ET, Eddy GA, Isaacson M, Johnson KM, McCormick JB, Murphy FA, Pattyn SR, Peters D, Prozesky OW, Regnery RL, Simpson DI, Slenczka W, Sureau P, van der Groen G, Webb PA, Wulff H (1982) Filoviridae: a taxonomic home for Marburg and Ebola viruses? Intervirology 18:24–32.CrossRefPubMedGoogle Scholar
  24. Kuzmin IV, Orciari LA, Arai YT, Smith JS, Hanlon CA, Kameoka Y, Rupprecht CE (2003) Bat lyssaviruses (Aravan and Khujand) from Central Asia: phylogenetic relationships according to N, P, G gene sequences. Virus Res 97:65–79.CrossRefPubMedGoogle Scholar
  25. Kuzmin IV, Hughes GJ, Botvinkin AD, Orciari LA, Rupprecht CE (2005) Phylogenetic relationships of Irkut and West Caucasian bat viruses within theLyssavirus genus and suggested quantitative criteria based on the N gene sequence for lyssavirus genotype definition. Virus Res 111:28–43.CrossRefPubMedGoogle Scholar
  26. Langevin P, Barclay RMR (1990)Hypsignathus monstrosus. Mammal Species 357:1–4.CrossRefGoogle Scholar
  27. Leirs H, Mills JN, Krebs JW, Childs JE, Akaibe D, Woollen N, Ludwig G, Peters CJ, Ksiazek TG (1999) Search for the Ebola virus reservoir in Kikwit Democratic Republic of the Congo: reflections on a vertebrate collection. J Infect Dis 179:S155–S163.CrossRefPubMedGoogle Scholar
  28. Leroy EM, Rouquet P, Formenty P, Souquiere S, Kilbourne A, Froment JM, Bermejo M, Smit S, Karesh W, Swanepoel R, Zaki SR, Rollin PE (2004a) Multiple Ebola virus transmission events and rapid decline of central African wildlife. Science 303:387–390.CrossRefPubMedGoogle Scholar
  29. Leroy EM, Telfer P, Kumulungui B, Yaba P, Rouquet P, Roques P, Gonzalez JP, Ksiazek TG, Rollin PE, Nerrienet E (2004b) A serological survey of Ebola virus infection in central African nonhuman primates. J Infect Dis 190:1895–1899.CrossRefPubMedGoogle Scholar
  30. Leroy EM, Kumulungui B, Pourrut X, Rouquet P, Hassanin A, Yaba P, Delicat A, Paweska JT, Gonzalez JP, Swanepoel R (2005) Fruit bats as reservoirs of Ebola virus. Nature 438:575–576.CrossRefPubMedGoogle Scholar
  31. Li W, Shi Z, Yu M, Ren W, Smith C, Epstein JH, Wang H, Crameri G, Hu Z, Zhang H, Zhang J, McEachern J, Field H, Daszak P, Eaton BT, Zhang S, Wang LF (2005) Bats are natural reservoirs of SARS-like coronaviruses. Science 310(5748):676–679.CrossRefPubMedGoogle Scholar
  32. Mackenzie JS (2005) Emerging zoonotic encephalitis viruses: lessons from Southeast Asia and Oceania. J Neurovirol 1:434–440.CrossRefGoogle Scholar
  33. Martini GA, Siegert R (1971) Marburg virus disease. Springer, Berlin Heidelberg New York, pp 131.Google Scholar
  34. Miranda ME, Ksiazek TG, Retuya TJ, Khan AS, Sanchez A, Fulhorst CF, Rollin PE, Calaor AB, Manalo DL, Roces MC, Dayrit MM, Peters CJ (1999) Epidemiology of Ebola (subtype Reston) virus in the Philippines, 1996. J Infect Dis 179 [Suppl 1]:S115–S119.CrossRefGoogle Scholar
  35. Monath TP (1999) Ecology of Marburg and Ebola viruses: speculations and directions for the future research. J Infect Dis 179:S127–S138.CrossRefPubMedGoogle Scholar
  36. Morvan JM, Deubel V, Gounon P, Nakoune E, Barriere P, Murri S, Perpete O, Selekon B, Coudrier D, Gautier-Hion A, Colyn M, Volehkov V (1999) Identification of Ebola virus sequences present as RNA or DNA in organs of terrestrial small mammals of the Central African Republic. Microbes Infect 1:1193–1201.CrossRefPubMedGoogle Scholar
  37. Morvan JM, Nakoune E, Deubel V, Colyn M (2000) Forest ecosystems and Ebola virus. B Soc Pathol Exot 93:172–175.Google Scholar
  38. Pinzon JE, Wilson JM, Tucker CJ, Arthur R, Jahrling PB, Formenty P (2004) Trigger events: enviroclimatic coupling of Ebola hemorrhagic fever outbreaks. Am J Trop Med Hyg 71:664–674.PubMedGoogle Scholar
  39. Pourrut X, Kumulungui B, Wittmann T, Moussavou G, Delicat A, Yaba P, Nkoghe D, Gonzalez JP, Leroy EM (2005) The natural History of Ebola virus in Africa. Microbes Infect 7:1005–1014.CrossRefPubMedGoogle Scholar
  40. Reiter P, Turell M, Coleman R, Miller B, Maupin G, Liz J, Kuehne A, Barth J, Geisbert J, Dohm D, Glick J, Pecor J, Robbins R, Jahrling P, Peters C, Ksiazek T (1999) Field investigations of an outbreak of Ebola hemorrhagic fever Kikwit Democratic Republic of the Congo, 1995: arthropod studies. J Infect Dis 179:S148–S154.CrossRefPubMedGoogle Scholar
  41. Rollin PE, Williams RJ, Bressler DS, Pearson S, Cottingham M, Pucak G, Sanchez A, Trappier SG, Peters RL, Greer PW, Zaki S, Demarcus T, Hendricks K, Kelley M, Simpson D, Geisbert TW, Jahrling PB, Peters CJ, Ksiazek TG (1999) Ebola (subtype Reston) virus among quarantined nonhuman primates recently imported from the Philippines to the United States. J Infect Dis 179 [Suppl 1]:S108–S114.CrossRefGoogle Scholar
  42. Shaman J, Day JF, Stieglitz M (2002) Drought-induced amplification of Saint Louis encephalitis virus Florida. Emerg Infect Dis 8:575–580.CrossRefPubMedGoogle Scholar
  43. Sulkin SE, Allen R (1974) Virus infections in bats. In: Melnick JL (ed) Monographs in Virolology 8. Karger, Basel.Google Scholar
  44. Swanepoel R, Leman PA, Burt FJ (1996) Experimental inoculation of plants and animals with Ebola virus. Emerg Infect Dis 2:321–325.CrossRefPubMedGoogle Scholar
  45. Walsh PD, Abernethy KA, Bermejo M, Beyers R, De Wachter P, Akou ME, Huijbregts B, Mambounga DI, Toham AK, Kilbourn AM, Lahm SA, Latour S, Maisels F, Mbina C, Mihindou Y, Obiang SN, Effa EN, Starkey MP, Telfer P, Thibault M, Tutin CE, White LJ, Wilkie DS (2003) Catastrophic ape decline in western equatorial Africa. Nature 422:611–614.CrossRefPubMedGoogle Scholar
  46. Walsh PD, Biek R, Real LA (2005) Wave-like spread of Ebola Zaire. PLoS Biol 3:e371.CrossRefPubMedGoogle Scholar
  47. Yob JM, Field H, Rashdi AM, Morrissy C, van der Heide B, Rota P, bin Adzhar A, White J, Daniels P, Jamaluddin A, Ksiazek T (2001) Nipah virus infection in bats (order Chiroptera) in peninsular Malaysia. Emerg Infect Dis 7:439–441.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2007

Authors and Affiliations

  • E. Leroy
    • 1
  • J. P. Gonzalez
    • 1
  • X. Pourrut
    • 1
  1. 1.Fundamentals and Domains of Disease Emergence Research UnitInstitute for Research Development, IRDParisFrance

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