Study of Endogenous Fluorescence as a Function of Tissues’ Conservation Using Spectral and Lifetime Measurements on Tumor or Epileptic Cortex Excision
Until today the endogenous fluorescence of tissue were neglected and often consider as a source of noise in medical imaging, however recent work and future technologies seems to reconsider it as a new imaging modality in medical devices. One of the precursor fields for the use of autofluorescence in tissue is the study of cancerology, which was recognized as a powerful tool for the future of medical devices. Although many studies have been started and done in this field, there are still numerous aspects of the signal that are not well known yet such as time dependence after extraction of fresh tissues. In this work, freshly resected human samples were exanimated in order to investigate their autofluorescence changes with time. Primary results of this examination prove that fluorescence intensity and lifetime values of healthy and tumoral samples decreased slightly with time.
KeywordsLifetime measurement Spectroscopy Autofluorescence Metastasis and cortical human samples
This Work as a part of the MEVO project was supported by “Plan Cancer” program founded by INSERM (France), by CNRS with “Défi instrumental” grant, and the Institut National de Physique Nucléaire et de Physique des Particules (IN2P3). Thanks to the PIMPA Platform partly funded by the French program “Investissement d’Avenir” run by the “Agence Nationale pour la Recherche” (grant “Infrastructure d’avenir en Biologie Santé – ANR – 11-INBS-0006”).
- 6.D.A. Hardesty, N. Sanai, The value of glioma extent of resection in the modern neurosurgical era. Front. Neurol. 3. https://doi.org/10.3389/fneur.2012.00140
- 7.N. Sanai, M.S. Berger, Glioma extent of resection and its impact on patient outcome. Neurosurgery 62, 753–766 (2008). https://doi.org/10.1227/01.neu.0000318159.21731.cfCrossRefGoogle Scholar
- 9.A.H. Zehri, W. Ramey, J.F. Georges, M.A. Mooney, N.L. Martirosyan, M.C. Preul, P. Nakaji, Neurosurgical confocal endomicroscopy: a review of contrast agents, confocal systems, and future imaging modalities. Surg. Neurol. Int. 5, 60 (2014). https://doi.org/10.4103/2152-7806.131638CrossRefGoogle Scholar
- 11.A. Chorvatova, D. Chorvat, Tissue fluorophores and their spectroscopic characteristics, ed. by L. Marcu, P. French, D. Elson, Fluorescence Lifetime Spectroscopy and Imaging (CRC Press, 2014), pp. 47–84Google Scholar
- 12.A.C. Croce, G. Bottiroli, Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis. Eur. J. Histochem. 58 (2014). https://doi.org/10.4081/ejh.2014.2461
- 14.M. Zanello, F. Poulon, P. Varlet, F. Chretien, F. Andreiuolo, M. Pages, A. Ibrahim, J. Pallud, E. Dezamis, G. Abi-Lahoud, F. Nataf, B. Turak, B. Devaux, D. Abi-Haidar, Multimodal optical analysis of meningioma and comparison with histopathology. J. Biophotonics (2016). https://doi.org/10.1002/jbio.201500251CrossRefGoogle Scholar
- 15.M. Zanello, A. Ibrahim, F. Poulon, P. Varlet, B. Devau, D. Abi Haidar, in Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS, vol. 1 (2016), pp. 13–17Google Scholar
- 16.D. Abi Haidar, B. Leh, A. Allaoua, A. Genoux, R. Siebert, M. Steffenhagen, D.A. Peyrot, N. Sandeau, C. Vever-Bizet, G. Bourg Heckly, I. Chebbi, Spectral and lifetime domain measurements of rat brain tumours, in SPIE (Ed.), Spectral and Lifetime Domain Measurements of Rat Brain Tumours (San Jose, United States, 2012), p. 82074P. https://doi.org/10.1117/12.907411
- 22.M.C. Skala, K.M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K.W. Eliceiri, J.G. White, N. Ramanujam, In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia. Proc. Natl. Acad. Sci. 104, 19494–19499 (2007)ADSCrossRefGoogle Scholar
- 24.M. Wolman, Lipid pigments (chromolipids): their origin, nature, and significance. Pathobiol. Annu. 10, 253 (1980)Google Scholar
- 25.S.A. Toms, W.-C. Lin, R.J. Weil, M.D. Johnson, E.D. Jansen, A. Mahadevan-Jansen, Intraoperative optical spectroscopy identifies infiltrating glioma margins with high sensitivity. Neurosurgery 61, 327–336 (2007). https://doi.org/10.1227/01.neu.0000279226.68751.21CrossRefGoogle Scholar
- 30.A.G. Ryder, S. Power, T.J. Glynn, J.J. Morrison, Time-domain measurement of fluorescence lifetime variation with pH, in BiOS 2001 The International Symposium on Biomedical Optics. International Society for Optics and Photonics (2001), pp. 102–109Google Scholar