Fabrication and Characterization of Enzymatic Electrochemical Glucose Sensor for Closed Loop System
Nanoparticle Cerium Oxide sputtered on Platinum coated on silicon dioxide gave promising results to be used as Glucose Biosensor for closed loop system. This stack of Sensor was then coated with Glucose Oxidase to form a working electrode of a three electrode system. Scattered electron microscope showed nanoporous surface morphology and cyclic voltammetry showed a linear trend in current for increase in Glucose concentration from 40 to 320 mg/dl in 10 ml Phosphate buffer solution. The current trend observed was from 50 to 120 µA. The results obtained show that cerium oxide can be ideally used for immobilizing Glucose Oxidase and hence can form an efficient part in Glucose Biosensor.
We, the authors would like to thank Indian Nanoelectronics User Program (INUP), Biosensor Lab, National Nanofabrication Facility (NNFC) and Micro and Nano Characterization facility (MNCF) at CENSE, Bangalore for providing necessary facilities required to carry out this work.