In Vitro Digestibility of Native Grass Silage Supplemented with Leucaena leucocephala

  • Ryan Aryadin Putra
  • Cuk Tri NoviandiEmail author
  • Nafiatul Umami
Conference paper


Research has been conducted to determine the best level of Leucaena leucocephala supplementation to digestibility and fermentability in vitro. The study was designed using completely randomized design with three levels supplementation of Leucaena (10%, 20%, and 30%) and each treatment was done in six replications. All of the silage treatments was inoculated with 2% Lactobacillus plantarum, and stored in mini-silos for 21 days. The results showed that supplementing native grass with 20% Leucaena resulted in the most significant (P < 0.05) dry matter and organic matter digestibility. The highest fiber crude digestibility was obtained at 30% supplementation. However, the highest digestibility of crude protein obtained at 10% Leucaena supplementation and the digestibility decreased linearly with the increasing levels of supplementation (P < 0.05). These data indicate that supplementing native grass with 20% Leucaena is the best treatment in terms of nutrient digestibility.


Leucaena leucocephala Lactobacillus plantarum In vitro Native grass Silage 



crude fiber


crude fiber digestibility


crude protein


crude protein digestibility


dry matter


dry matter digestibility


Ether Extract


in vitro crude fiber digestibility


in vitro crude protein digestibility


in vitro dry matter digestibility


in vitro organic matter digestibility


lactic acid bacteria


Lembaga Ilmu Pengetahuan Indonesia


Nitrogen free extract


organic matter


organic matter digestibility


polyethylene glycol


total digestible nutrient


Universitas Gadjah Mada



Thanks to Indonesia Endowment Fun for Education, the Ministry of Finance, The Republic of Indonesia as the main sponsor of this research, either in the financial support and the moral support. (Kep-105/LPDP/2014)


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Ryan Aryadin Putra
    • 1
  • Cuk Tri Noviandi
    • 1
    Email author
  • Nafiatul Umami
    • 1
  1. 1.Faculty of Animal ScienceUniversitas Gadjah MadaYogyakartaIndonesia

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