Biosorption of Metals Ion on Methanol Dehydrogenase Activity Test of Bradyrhizobium japonicum USDA110

  • Novita KurniawatiEmail author
  • Ambar Pertiwiningrum
  • Yuny Erwanto
  • Nanung Agus Fitriyanto
  • Mohammad Zainal Abidin
Conference paper


This research aimed to know effect of absorption metals ion (bisorption) which has different group elements on methanol dehydrogenase (MDH) activity test of a nitrogen fixation bacterium, Bradyhizobium japonicum USDA 110. Ten of metals ion at a concentration of 30 μM were tested to grow B. japonicum USDA 110 in diluted one per ten of nutrient medium. The results of MDH activity test showed that the bacterial growth in medium without added metal ion had similar MDH activity with that of in the medium supplemented with calsium ion/Ca2+. Highest enzymatic activity, 0.08 (U · mg−1), was showed by the bacterium grown in medium supplemented with magnesium/Mg2 + .. MDH was required for the bacterial growth in anerobic condition specially for a root simbiotic bacterium, Bradyhizobium japonicum USDA 110. Addition of magnesium/Mg2+ accelerated the bacterial growth and MDH activity by 2.6 and 1.28 times, respectively. This research results deduced that in an anerobic condition of soil, the metal ions specially calsium and magnesium were required to be added in plant growth medium to accelerate the growth of B. japonicum which well known as bacterial symbiotic on soy bean roots.


Biosorption Bradyrhizobium japonicum USDA110 Metal ion Methanol dehydrogenase Nutrient medium 



bovine serum albumin




Methanol Dehydrogenase


Nitro Blue Tetrazolium


Polyacrylamide Gel Electrophoresis


Phenazine Ethosulfate


Sodium Dodecyl sulfat


Yeast extrac-Mannitol



The author gratitude to the directorate General of Higher Education, Ministry of Research, Technology, and Higher Education of the Republic of Indonesia which endoswed her Master study program scholarship.


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Novita Kurniawati
    • 1
    Email author
  • Ambar Pertiwiningrum
    • 1
  • Yuny Erwanto
    • 1
  • Nanung Agus Fitriyanto
    • 1
  • Mohammad Zainal Abidin
    • 1
  1. 1.Departement of Animal Products Technology, Faculty of Animal ScienceUniversitas Gadjah MadaYogyakartaIndonesia

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