Physiological Responses of Rice (Oryza sativa L. ‘Situ Bagendit’) to Varying Water Stress and Soil Type

  • Diah RachmawatiEmail author
  • Fikky Amalia
Conference paper


Drought causes decreased growth and induced physiological responses. The objective of this research was to analyze the physiological responses of rice (Oryza sativa L. ‘Situ Bagendit’) to varying water stress and soil texture type. This research used a complete randomized design with two factors: (i) drought stress treatment of 100%, 50% and 25% of field capacity; (ii) soil texture type: clay, clay loam and sandy clay loam. The variables observed were plant heights, number of leaves, relative water content (RWC), plant biomass, chlorophyll and proline levels. Data were analyzed using ANOVA, followed with DMRT analysis (with α = 0.05). This research showed that drought decreased RWC, plant heights, and plant biomass. Drought caused accumulation of proline levels as compatible solute that regulates osmotic balance. Soil media with sandy clay loam texture showed optimal growth based on plant height, number of leaves and shoot biomass. The interaction between drought and soil type showed significant differences in relative water content, total chlorophyll and proline levels.


Drought Proline Rice RWC Soil type 



Relative Water Content


  1. 1.
    Indonesian Center for Rice Research. Situ Bagendit [Internet]. Indonesia: Balai Besar Penelitian Tanaman Padi; 2017 [cited 2017 February 7th]. Available from:
  2. 2.
    Parker R. Plant and soil science: fundamentals and application. Clifton Park, NY, USA: Delmar Cencage Laerning, Inc.; 2009. p. 112–4.Google Scholar
  3. 3.
    Biswas TD, Mukherjee SK. Textbook of soil science. New Delhi: McGraw-Hill Publishing Company; 2001. p. 162–4.Google Scholar
  4. 4.
    Karmollachaab A, Gharineh MH. Effect of silicon apllication on wheat seedlings growth under water-deficit stress induced by polyethylene glycol. Iran Agr Res. 2015;34(1):31–8.Google Scholar
  5. 5.
    Cattivelli L, Rizza F, Badeck FW, Mazzucotelli E, Mastrangelo AM, Francia E, et al. Drought tolerance improvement in crop plants: an integrated view from breeding to genomics. Field Crop Res. 2008;105:1–14.CrossRefGoogle Scholar
  6. 6.
    Izanloo A, Condon AG, Langridge P, Tester M, Schnurbusch T. Different mechanism on adaptation to cyclic water stress in two south Australian bread wheat cultivar. J Exp Bot. 2008;59(12):3327–46.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Blum A. Drought resistance, water-use efficiency, and yield potential: are they compatible, dissonant, or mutually exclusive? Aust J Agric. 2005;56:1159–68.CrossRefGoogle Scholar
  8. 8.
    Da Costa M, Huang B. Osmotic adjustment associated with variation in bentgrass tolerance to drought stress. J Am Soc Hortic Sci. 2006;131:338–44.Google Scholar
  9. 9.
    Bates L, Waldren RP, Teare ID. Rapid determination of free proline for water stress studies. In: Roger MJR, editor. Handbook of plant ecophysiology techniques. New York: Kluwer Academic Publishers; 1973. p. 374–8.Google Scholar
  10. 10.
    Yoshida S, Forno D, Cock J, Gomez KA. Laboratory manual for physiological studies of rice. Los Banos: IRRI; 1976.Google Scholar
  11. 11.
    Gupta US. Production and improvement of crops for dryland. New York: Science Publishers, Inc.; 1995. p. 30–2.Google Scholar
  12. 12.
    Rahayu AY, Harjoso T. Karakter agronomis dan fisiologis padi gogo yang ditanam pada media tanah bersekam pada kondisi air di bawah kapasitas lapang [Agronomical and physiological characters of upland rice grown in soil-rice hull media under lower field capacity]. Akta Agrosia. 2010;13(1):40–9. [in Bahasa Indonesia]Google Scholar
  13. 13.
    Jeki. Indeks sensitifitas stres beberapa varietas padi gogo pada cekaman kekeringan [Sensitifity stress indeks of upland rice under drought stress]. Jurnal Agrotekbis. 2016;4(4):369–73. [in Bahasa Indonesia]Google Scholar
  14. 14.
    Chutia J, Borah SP. Water stress on leaf growth and chlorophyll content but not the grain yield in traditional rice (Oryza sativa Linn.) genotype of Assam, India II. Protein and proline status in seedlings under PEG induced water stress. Am J Plant Sci. 2012;3:971–80.CrossRefGoogle Scholar
  15. 15.
    Lin J, Wang G. Doubled CO2 could improved the drought tolerance better in sensitive cultivar than in tolerant cultivars in spring wheat. Plant Sci. 2002;163:627–37.CrossRefGoogle Scholar
  16. 16.
    Murningsih T, Yulita KS, Bora CY, Arsa IGBA. Respon tanaman jagung varietas lokal NTT umur sangat genjah (Pena Tunu’Ana’) terhadap cekaman kekeringan [Response of maize landrace NTT with very early maturity (Pena Tunu’ Ana’) to drought stress]. Berita Biologi. 2015;14(1):49–55. [in Bahasa Indonesia]Google Scholar
  17. 17.
    Ronde JA, Mescht VD, Steyin HS. Proline accumulation in response to drought and heat stress in cotton. Afr Crop Sci J. 2000;8:85–91.CrossRefGoogle Scholar
  18. 18.
    Zhang T, Yu LX, Zheng P, Li Y, Rivera M, Main D, et al. Identification of loci associated with drought resistance traits in heterozygous autotetraploid alfalfa (Medicago sativa L.) using genome-wide association studies with genotyping by sequencing. PLoS One. 2015;10(9):1–17.Google Scholar

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© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Faculty of BiologyUniversitas Gadjah MadaYogyakartaIndonesia

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