Systematic Position, Phylogeny, and Taxonomic Revision of Indian Ocimum

  • Amit Kumar
  • Ashutosh K. Shukla
  • Ajit Kumar Shasany
  • Velusamy SundaresanEmail author
Part of the Compendium of Plant Genomes book series (CPG)


The genus Ocimum belongs to the family Lamiaceae, and most popular herb/shrub known for its immense value belongs to their medicinal properties and aroma profile. The presence of vast morphological as well as chemical variability within the genus, its taxonomy, and phylogenetic relationships is still debatable. Adding to this, chemotypes within the species, which are more or less similar in morphology, increases the complexity to higher level. Nowadays, the use of molecular markers, viz. PCR and sequence-based markers, became an essential tool to assess interspecific relationships as they are highly efficient and provide more variable and informative insight into evolutionary rates. The present article presents the detailed taxonomic description as well as phylogenetic relationships among the Indian Ocimum species.


Lamiaceae Tulsi Ocimum Systematics Phylogeny 



Director, CSIR-CIMAP, Lucknow, is duly acknowledged for providing the laboratory facilities required to carry out this research. Authors also express their gratitude for the financial support from the Council of Scientific and Industrial Research (CSIR), New Delhi, through the XIIth FYP project ChemBio (BSC-0203).


  1. Backer CA, Bakhuizen van den Brink RC (1965) Lamiaceae, Flora of Java. NVP Noordhoff, Groningen, pp 614–640Google Scholar
  2. Balyan SS, Pushpangadan P (1988) A study on the taxonomical status and geographic distribution of genus Ocimum. Pafai J 10(2):13–19Google Scholar
  3. Bentham G (1830) Synopsis of the Genera and Species of Indian Labiatae enumerated in the Catalogue of the Collection in Dr. Wallich’s charge. In: Wallich N (ed) Plantae Asiaticae Rariores. Treuttel and Wurtz, London, pp 12–19Google Scholar
  4. Bentham G (1832) Labiatarum Genera et Species 1. James Ridgway and Sons, London, pp 1–19Google Scholar
  5. Bentham G (1848) Ocimoideae. In: de Candolle AP (ed) Prodromus Systematis Naturalis Regni Vegetabilis. Victor Masson, Paris, pp 30–148Google Scholar
  6. Bentham G (1876) Ocimoideae. In: Bentham G, Hooker JD (eds) Genera Plantarum. Reeve and Co, London, pp 1162–1164, 1171–1179Google Scholar
  7. Briquet J (1897) Labiatae. In: Engler A, Prantl K (eds) Die Natuirlichen Pflanzenfamilien. Wilhelm Engelmann, Leipzig, pp 183–375Google Scholar
  8. Budantsev AL (1999) The Family Lamiaceae in Vietnam. Komarovia pp 3–33Google Scholar
  9. Carovic K, Liber Z, Javornik B, Kolak I, Satovic Z (2006) Genetic relationships within Basil (Ocimum) as revealed by RAPD and AFLP markers. Acta Hortic 760:171Google Scholar
  10. Carovic-Stanko K, Orlic S, Politeo O, Strikic F, Kolak I, Milos M, Satovic Z (2010) Composition and antibacterial activities of essential oils of seven Ocimum taxa. Food Chem 119:196–201CrossRefGoogle Scholar
  11. Carvoic-Stanko K, Liber Z, Besendorfer V, Javornik B, Bohanec B, Kolak I, Satovic Z (2010) Genetic relations among basil taxa (Ocimum L.) based on molecular markers, nuclear DNA content, and chromosome number. Plant Syst Evol 285:13–22CrossRefGoogle Scholar
  12. Carvoic-Stanko K, Liber Z, Politeo O, Strikic F, Kolak I, Milos M, Satovic Z (2011) Molecular and chemical characterization of the most widespread Ocimum species. Plant Syst Evol 294:253-262CrossRefGoogle Scholar
  13. Charles DJ, Simon JE (1992) A new geraniol chemotype of Ocimum gratissimum L. J Essent Oil Res 4:231–234CrossRefGoogle Scholar
  14. Chen SY, Dai TX, Chang YT, Wang SS, Ou SL, Chuang WL, Chuang CY, Lin YH, Lin YH, Ku HM (2013) Genetic diversity among Ocimum species based on ISSR, RAPD and SRAP markers. Aust J Crop Sci 7(10):1463–1471Google Scholar
  15. Clement RA (1999) Labiatae. In: Long DG (ed) Flora of Bhutan. Royal Botanic Garden, Edinburgh, pp 938–1002Google Scholar
  16. Cramer LH (1981) Lamiaceae (Labiatae). In: Dassanayake MD (ed) A revised handbook to the flora of ceylon. Oxford and IBH Publishing Co, New Delhi, pp 108–194Google Scholar
  17. Doan T (1936) Labiatae. In: Lecomte H (ed) Flore Generale de L. Indo-Chine, Masson, Paris, pp 915–1046Google Scholar
  18. Forsskål P (1775) Flora aegyptiaco-arabica. Moller, KjobenhavnGoogle Scholar
  19. Grayer RJ, Kite GC, Goldstone FJ, Bryan SE, Paton A, Putievsky E (1996) Intraspecific taxonomy and essential oil chemotypes in sweet basil, Ocimum basilicum. Phytochem 43:1033–1039CrossRefGoogle Scholar
  20. Gurke M (1895) Labiatae. In: Engler A (ed) Die Pflanzenwelt Ostafrikas. Teil C Dietrich Reimer, Berlin, pp 337–342Google Scholar
  21. Hakkim FL, Arivazhagan G, Boopathy R (2008) Antioxidant property of selected Ocimum species and their secondary metabolite content. J Med Plants Res 2(9):250–257Google Scholar
  22. Harley MM, Paton A, Harley RM, Cade PG (1992) Pollen morphological studies in tribe Ocimeae (Nepetoideae: Labiatae): I. Ocimum L. Grana 31:161–176CrossRefGoogle Scholar
  23. Ho PH (1993) Labiatae. In: Ho PH (ed) An illustrated flora of Vietnam. Mekong Pring, Santa Ana, pp 1065–1108Google Scholar
  24. Hooker JD (1885) Labiatae. Flora of British India. Reeve and Co, London, pp 604–705Google Scholar
  25. Jacquin NJ (1792) Icones plantarum rariorum. Wappler CF, Vienna. White, LondonGoogle Scholar
  26. Keng H (1969) Flora Malesianae precursors. A revision of Malesian Labiatae. Gard Bull, Singapore, pp 13–180Google Scholar
  27. Keng H (1978) Labiatae. In: van Steenis CGGJ (ed) Flora Malesiana ser. Sijthoff and Noordhoff, Alpen Aan Den Rijn, pp 301–394Google Scholar
  28. Keng H (1990) The concise flora of Singapore: gymnosperms and dicotyledons. Singapore University Press, SingaporeGoogle Scholar
  29. Khosla MK (1995) Study of inter-relationship, phylogeny and evolutionary tendencies in genus Ocimum. Indian J Genet Plant Breed 55:71–83Google Scholar
  30. Kokwaro JO (1976) Medical Plants of East Africa. East African Literature Bureau, General Printers Limited, Nairobi, p 384Google Scholar
  31. Kudo Y (1929) Labiatarum sino-japonicarum prodromus. Memoirs of the faculty of science and agriculture. Taihoku Imperial University, Taiwan, pp 37–332Google Scholar
  32. Kumar A, Mishra P, Baskaran K, Shukla AK, Shasany AK, Sundaresan V (2016) Higher efficiency of ISSR markers over plastid psbA-trnH region in resolving taxonomical status of genus Ocimum L. Ecol Evol 6:7671–7682CrossRefGoogle Scholar
  33. Li XW (1977) Lamiaceae. In: Wu CY, Li HW (eds) Flora Reipublicae Popularis Sinicae. Academica Sinica, ChinaGoogle Scholar
  34. Li XW, Hedge I (1994) Labiatae. In: Wu ZY, Raven PH (eds) Flora of China. Missouri Botanical Garden, Missouri, pp 298–300Google Scholar
  35. Linnaeus C (1753) Species Plantarum. Laurentii Salvii, HolmiaeGoogle Scholar
  36. Linnaeus C (1754) Genera Plantarum (ed). Laurentii Salvii, HolmiaeGoogle Scholar
  37. Loureiro J (1790) Flora Cochinchinensis. Ulyssipone, pp 357–375Google Scholar
  38. Mattia FD, Bruni I, Galimberti A, Cattaneo F, Casiraghi M, Labra M (2011) A comparative study of different DNA barcoding markers for the identification of some members of Lamiacaea. Food Res Int 44:693–702CrossRefGoogle Scholar
  39. Merrill ED (1935) A commentary on Loureiro’s “Flora cochinchinensis”. Trans Am Phil Soc 1–445CrossRefGoogle Scholar
  40. Mukerjee SK (1940) A revision of the Labiatae of the Indian Empire. Rec Bot Surv India 14:1–228Google Scholar
  41. Murata G (1971) Contributions to the Flora of Southeast Asia IV: a list of Labiatae known from Thailand. South E Asian Stud 8:489–517Google Scholar
  42. Muschler R, Hosseus CC (1910) Beitrage zur Flora Siams. Beih Bot Centralbl 27:455–507Google Scholar
  43. Obeng-Ofori D, Reichmuth CH, Bekele AJ, Hassanali A (1998) Toxicity and protectant potential of camphor, a major component of essential oil of Ocimum kilimandscharicum, against four stored product beetles. Int J Pest Manag 44:203–209CrossRefGoogle Scholar
  44. Patel HK, Fougat RS, Kumar S, Mistry JG, Kumar M (2015) Detection of genetic variation in Ocimum species using RAPD and ISSR markers. 3. Biotech 5:697–707Google Scholar
  45. Paton AJ (1992) A synopsis of Ocimum L. in Africa. Kew Bull 47:405–437Google Scholar
  46. Paton AJ, Putievsky E (1996) Taxonomic problems and cytotaxonomic relationships between and within varieties of Ocimum basilicum and related species (Labiatae). Kew Bull 51:509–524CrossRefGoogle Scholar
  47. Paton AJ, Harley RM, Harley MM (1999) Ocimum: an overview of classification and relationships. In: Hiltunen R, Holm Y (eds) Ocimum. medicinal and aromatic plants—industrial profiles. Harwood Academic, Amsterdam, pp 1–38Google Scholar
  48. Phuong VX (1982) Conspectus Lamiacearum Florae Vietnam. Novosti Sist Nizsh Rast 19:125–160Google Scholar
  49. Phuong VX (1995) The Lamiaceae in the Flora of Vietnam. J Biol 17:33–46Google Scholar
  50. Phuong VX (2000) Lamiaceae (Labiatae). In: Ban NT, Ly TD, Khanh TC, Loc PK, Thin NN, Tien NV, Khoi NK (eds) Flora of Vietnam. Science and Technics Publishing House, Hanoi, pp 1–278Google Scholar
  51. Pino JA, Rosado A, Fuentes V (1996) Composition of the essential oil from the leaves and flowers of Ocimum gratissimum L. grown in Cuba. J Essen Oil Res 8:139–141CrossRefGoogle Scholar
  52. Prasad G, Kumar A, Singh AK, Bhattacharya AK, Singh K, Sharma VD (1986) Antimicrobial activity of essential oils of some Ocimum species and clove oil. Fitoterapia 57:429–432Google Scholar
  53. Press JR (1982) Elsholtzia. In: Hara H, Chater AO, Williams LHJ (eds) An enumeration of the flowering plants of Nepal. British Museum (Natural History), LondonGoogle Scholar
  54. Pushpangadan P, Bradu BL (1995) Medicinal and aromatic plants. In: Chadha KL, Gupta R (eds) Advances in horticulture. Malhotra Publishing House, New Delhi, pp 627–657Google Scholar
  55. Rastogi S, Kalra A, Gupta V, Khan F, Lal RK, Tripathi AK, Parameswaran S, Gopalakrishnan C, Ramaswamy G, Shasany AK (2015) Unravelling the genome of Holy basil: an “incomparable” “elixir of life” of traditional Indian medicine. BMC Genom 16:413CrossRefGoogle Scholar
  56. Ridley HN (1923) Labiatae. In: Ridley HN (ed) The flora of the Malay Peninsula. Reeve and Co, London, pp 624–655Google Scholar
  57. Simon JE, Quinn J, Murray RG (1990) Basil: a source of essential oils. In: Janick J, Simon JE (eds) Advances in new crops. Timber Press, Portland, pp 484–989Google Scholar
  58. Singh RK, Dwivedi RS (1987) Effect of oils on Sclerotium rolfsii causing foot rot of barley. Indian Phytopathol 40:531–533Google Scholar
  59. Singh AP, Dwivedi S, Bharti S, Srivastava A, Singh V, Khanuja SPS (2004) Phylogenetic relationships as in Ocimum revealed by RAPD markers. Euphytica 136:11–20CrossRefGoogle Scholar
  60. Sobti SN, Pushpangadan P (1979) Cytotaxonomical studies in the genus Ocimum. In: Bir SS (ed) Taxonomy, cytogenetics and cytotaxonomy of plants. Kalyani Publication, New Delhi, pp 373–377Google Scholar
  61. Suddee S, Paton AJ, Parnell JAN (2005) Taxonomic revision of tribe Ocimeae Dumort. (Lamiaceae) in Continental South East Asia III. Ociminae. Kew Bull 60:3–75Google Scholar
  62. Tadros MG, Ezzat SM, Salama MM, Farag MA (2014) In vitro and in vivo Anticholinesterase activity of the volatile oil of the aerial parts of Ocimum basilicum L. and Ocimum africanum Lour. Growing in Egypt. Int J Med Health Biomed Bioeng Pharm Eng 8(3):157–161Google Scholar
  63. Tokin B (1944) Phytoncides or plant bactericides, upon protozoa. Am Rev Soviet Med 1:237–239Google Scholar
  64. Weaver DK, Dunkel FV, Ntezurubanza L, Jackson LL, Stock DT (1991) The efficacy of linalool, a major component of freshly-milled Ocimum canum Sims (Lamiaceae), for protection against postharvest damage by certain stored product Coleoptera. J Stored Prod Res 27:213–220CrossRefGoogle Scholar
  65. Verma RS, Kumar A, Mishra P, Baskaran K, Padalia RC, Sundaresan V (2016) Essential oil composition of four Ocimum spp. from the Peninsular India. J Essen Oil Res 28:35–41CrossRefGoogle Scholar
  66. Vina A, Murillo E (2003) Essential oil composition from twelve varieties of basil (Ocimum spp.) grown in Colombia. J Braz Chem Soc 14:744–749CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Amit Kumar
    • 1
  • Ashutosh K. Shukla
    • 2
  • Ajit Kumar Shasany
    • 2
  • Velusamy Sundaresan
    • 1
    Email author
  1. 1.Plant Biology and SystematicsCSIR—Central Institute of Medicinal and Aromatic Plants, Research CentreBengaluruIndia
  2. 2.Biotechnology DivisionCSIR—Central Institute of Medicinal and Aromatic PlantsLucknowIndia

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