Biofuel Production from Carbon Dioxide Gas in Polluted Areas
Although carbon dioxide (CO2) in the air is at a low level (between 0 and 0.03%), the concentration of it is significantly higher in industrial regions. The CO2 concentration in the atmosphere increases 2–3 ppm every year because of the burning of fossil fuels. Global studies have focused on reducing the carbon dioxide level to the minimum limit (450 ppm) by reducing CO2 emissions 50–80% by the year 2050. In this study, in order to minimize the CO2 levels in the Aliağa and Atatürk industrial districts in Izmir, Turkey, S. elongatus from cyanobacteria were isolated from the Gölcük Lake in Ödemiş, Izmir, and were used to produce 1-butanol from CO2 via photosynthesis as a fuel source, instead of gasoline, for cars. The maximum 1-butanol concentration produced was 79 mg/L, and the 1-butanolproduced/CO2utilized efficiency was 87.6% in the S. elongatus species isolated from the Gölcük Lake at a temperature of 30 °C, at 60 W light intensity, at pH = 7.1, at 120 mV redox potential, at a flow rate of 0.083 m3/min using CO2 from the Aliağa industrial region, and at 0.5 mg/L dissolved O2 concentration. The maximum 1-butanol concentration produced was 59 mg/L, and the 1-butanolproduced/CO2utilized efficiency was 67.9% in the Atatürk industrial district due to low levels of polluted air in this region. In order to produce 10.000 m3 1-butanol from 1000 g/L CO2, the cost was calculated as 0.13 euro, while the addition of plasmid increased the cost to 0.66 euro to produce 10.000 m3 1-butanol.
Keywords1-Butanol CO2 S. elongatus Cyanobacteria Biofuel
This study was prepared in the scope of master of science studies in biotechnology and at the same time it was supported by the DEU Scientific Research Foundation (2014.KB.FEN.035) in Dokuz Eylul University Graduate School of Natural and Applied Sciences. In addition, the author acknowledges The Scientific and Technological Research Council of Turkey (TUBİTAK) for the financial support to the project numbered 114Y72.
- 1.Microbe Wiki (2014) Title of Synechococcus. https://microbewiki.kenyon.edu/index.php/Synechococcus. Accessed 15 Apr 2014
- 2.Armbrust EV, Bowen JD, Olson RJ, Chisholm SW (1989) Effect of light on the cell cycle of a marine synechococcus strain. Appl Environ Microbiol 55(2):425–432Google Scholar
- 4.Devaki B, Watanabe N, Ogawa T (1999) National center for biotechnology information. U.S. National Library of Medicine 96(6):3188–3319Google Scholar
- 5.Binder BJ, Chisholm SW (1995) Cell cycle regulation in marine synechococcus sp. strains. Appl Environ Microbiol 61(2):708–717Google Scholar
- 7.Denhez F (2007) Global climate change and its effects in Turkey. Turkey, p 56–58Google Scholar
- 19.Maeda S, Kawaguchi Y, Ohe TA, Omata T (1998) Cis-acting sequences required for NtcB-dependent, nitrite-responsive positive regulation of the nitrate assimilation operon in the cyanobacterium synechococcus sp. strain PCC 7942. J Bacteriol 180:4080–4088Google Scholar
- 20.Standard methods for water and wastewater engineering (2012) APHA – AWWA, USAGoogle Scholar
- 22.NCBI (2014) U.S. National Library of Medicine. https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=1129&lvl=3&lin=f&keep=1&srchmode=1&unlock. Accessed 15 Apr 2014
- 26.Yan R, Zhang Z, Zhu D (2009) Carbon and energetic metabolism of synechococcus sp. PCC7942 under photoautotrophic conditions. Springer Science and Business Media 25(9):1352–1359Google Scholar
- 27.Rippka R, Deruelles J, Waterbury JB, Herdman M, Stainer RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61Google Scholar