Fluorescein Angiography

  • Stephen H. Tsang
  • Tarun SharmaEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1085)


Fluorescein angiography (FA) is an imaging modality used to study the circulation of the retina and choroid. About 5 mL of 10% sterile aqueous solution of fluorescein dye (or 2–3 mL of a 25% solution) is injected in the antecubital vein. The fluorescein molecules excited by a blue light (465–490 nm) emit green-yellow light (520–530 nm) (Fig. 2.1). The reflected blue light and green-yellow light are passed through a barrier filter that allows only the green-yellow fluorescent light to pass through and blocks the blue reflected light.


Fluorescein Angiography 

Suggested Reading

  1. Berkow JW, Flower RW, Orth DH, Kelley JS. Fluorescein and indocyanine green angiography. 2nd ed. San Francisco: American Academy of Ophthalmology; 1997.Google Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Jonas Children’s Vision Care, Bernard & Shirlee Brown Glaucoma Laboratory, Columbia Stem Cell Initiative-Departments of Ophthalmology, Biomedical Engineering, Pathology & Cell Biology, Institute of Human Nutrition, Vagelos College of Physicians and Surgeons, Columbia UniversityNew YorkUSA
  2. 2.Department of Ophthalmology, Columbia UniversityEdward S. Harkness Eye Institute, NewYork-Presbyterian HospitalNew YorkUSA

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