Root Morphology

  • Antonio Lupini
  • Fabrizio Araniti
  • Antonio Mauceri
  • Maria Princi
  • Antonino Di Iorio
  • Agostino Sorgonà
  • Maria Rosa AbenavoliEmail author


Root system defined as the “Hidden Half” of plant, has not attracted a great deal of attention for a long time from plant biologists. In recent years, through the new innovative techniques, root system has been deeply studied allowing all to reveal its structure, function, but also its genetic potential, which could be manipulated to improve crop yield and plant survival in stressful environments. Plant root system has three major functions: site of water and nutrients acquisition from the soil, essential support for plant anchoring and sensor of abiotic and biotic stresses. It also points out secondary functions such as photoassimilates storage, phytohormones synthesis and clonal propagation.


  1. Abenavoli MR, Leone M, Sunseri F, Bacchi M, Sorgonà A (2016) Root phenotyping for drought tolerance in bean landraces from Calabria (Italy). J Agric Crop Sci 202:1–12CrossRefGoogle Scholar
  2. Adu MO, Chatot A, Wiesel L, Bennett MJ, Broadley MR, White PJ, Dupuy LX (2014) A scanner system for high-resolution quantification of variation in root growth dynamics of Brassica rapa genotypes. J Exp Bot 65:2039–2048CrossRefPubMedPubMedCentralGoogle Scholar
  3. Arsenault JL, Pouleur S, Messier C, Guay R (1995) WinRhizo, a root measuring system with a unique overlap correction method. Hortic Sci 30:906Google Scholar
  4. Berntson GM (1994) Root systems and fractals: how reliable are calculations of fractal dimension? Ann Bot 73:281–284CrossRefGoogle Scholar
  5. Costa C, Dwyer LM, Dutilleul P, Foroutan-pour K, Liu A, Hamel C, Smith DL (2003) Morphology and fractal dimension of root systems of maize hybrids bearing the leafy trait. Can J Bot 81:706–713CrossRefGoogle Scholar
  6. Danjon F, Sinoquet H, Godin C, Colin F, Drexhage M (1999) Characterisation of structural tree root architecture using 3D digitising and AMAPmod software. Plant Soil 211:241–258CrossRefGoogle Scholar
  7. Di Iorio A, Lasserre B, Scippa GS, Chiatante D (2005) Root system architecture of Quercus pubescens trees growing on different sloping conditions. Ann Bot 95:351–361CrossRefPubMedGoogle Scholar
  8. Eghball B, Settimi JR, Maranville JW, Parkhurst AM (1993) Fractal analysis for morphological description of corn roots under nitrogen stress. Agron J 85:287–289CrossRefGoogle Scholar
  9. Eshel A (1998) On the fractal dimensions of a root system. Plant Cell Environ 21:247–251CrossRefGoogle Scholar
  10. Fang S, Yan X, Liao H (2009) 3D reconstruction and dynamic modeling of root architecture in situ and its application to crop phosphorus research. Plant J 60:1096–1108CrossRefPubMedGoogle Scholar
  11. Fitter AH (1991) The ecological significance of root system architecture: an economic approach. In: Atkinson D (ed) Plant root growth: an ecological perspective. Blackwell Scientific, Oxford, pp 229–243Google Scholar
  12. Fitter AH (1996) Characteristics and functions of root system. In: Waisel Y, Eshel A, Kafkafi U (eds) Plant roots, the hidden half. Marcel Dekker, New York, pp 1–20Google Scholar
  13. Fitter AH, Stickland TR (1992) Fractal characterization of root system architecture. Funct Ecol 6:632–635CrossRefGoogle Scholar
  14. Forde BG, Lorenzo H (2001) The nutritional control of root development. Plant Soil 232:51–68CrossRefGoogle Scholar
  15. Hagrey SA (2007) Geophysical imaging of root-zone, trunk, and moisture heterogeneity. J Exp Bot 58:839–854CrossRefGoogle Scholar
  16. Hargreaves C, Gregory P, Bengough A (2009) Measuring root traits in barley (Hordeum vulgare ssp. vulgare and ssp. spontaneum) seedlings using gel chambers, soil sacs and X-ray microtomography. Plant Soil 316:285–297CrossRefGoogle Scholar
  17. Hochholdinger F, Zimmermann R (2008) Conserved and diverse mechanisms in root development. Curr Opin Plant Biol 11:70–74CrossRefPubMedGoogle Scholar
  18. Hochholdinger F, Woll K, Sauer M, Dembinsky D (2004) Genetic dissection of root formation in maize (Zea mays L.) reveals root-type specific developmental programmes. Ann Bot 93:359–369CrossRefPubMedPubMedCentralGoogle Scholar
  19. Hruska J, Cermak J, Sustek S (1999) Mapping of tree root systems by means of the ground penetrating radar. Tree Physiol 19:125–130CrossRefPubMedGoogle Scholar
  20. Iyer-Pascuzzi AS, Symonova O, Mileyko Y, Yueling H, Belcher H, Harer J, Weitz JS, Benfey PN (2010) Imaging and analysis platform for automatic phenotyping and trait ranking of plant root systems. Plant Physiol 152:1148–1157CrossRefPubMedPubMedCentralGoogle Scholar
  21. Jahnke S, Menzel MI, Van Dusschoten D, Roeb GW, Buhler J, Minwuyelet S, Blumler P, Temperton VM, Hombach T, Streun M (2009) Combined MRI–PET dissects dynamic changes in plant structures and functions. Plant J 59:634–644CrossRefPubMedGoogle Scholar
  22. Le Marie C, Kirchgessner N, Marschall D, Walter A, Hund A (2014) Rhizoslides: paper-based growth system for non-destructive, high throughput phenotyping of root development by means of image analysis. Plant Methods 10:10–13CrossRefGoogle Scholar
  23. Loudet O, Gaudon V, Trubuil A, Daniel-Vedele F (2005) Quantitative trait loci controlling root growth and architecture in Arabidopsis thaliana confirmed by heterogeneous inbred family. Theor Appl Genet 110:742–753CrossRefPubMedGoogle Scholar
  24. Lynch JP (1995) Root architecture and plant productivity. Plant Physiol 109:7–13CrossRefPubMedPubMedCentralGoogle Scholar
  25. Lynch JP, Nielsen KL (1996) Simulation of root system architecture. In: Waisel Y, Eshel A, Kafkafi U (eds) Plant roots, the hidden half. Marcel Dekker, New York, pp 247–257Google Scholar
  26. Lynch JP, van Beem JJ (1993) Growth and architecture of seedling root of common bean genotypes. Crop Sci 33:1253–1257CrossRefGoogle Scholar
  27. Malamy JE (2009) Lateral root formation. In: Beeckman T (ed) Root development. Wiley Online Library, pp 83–126Google Scholar
  28. Muday GK, Rahman A (2007) Auxin transport and the integration of gravitropic growth. In: Gilroy S, Masson P (eds) Plant tropisms. Blackwell Publishing, Oxford, pp 47–78CrossRefGoogle Scholar
  29. Newman EI, Andrews RE (1973) Uptake of phosphorus and potassium in relation to root growth and root density. Plant Soil 38:49–69CrossRefGoogle Scholar
  30. Nibau C, Gibbs D, Coates J (2008) Branching out in new directions: the control of root architecture by lateral root formation. New Phytol 179:595–614CrossRefPubMedGoogle Scholar
  31. Oppelt AL, Kurth W, Dzierzon H, Jentschke G, Godbold DL (2000) Structure and fractal dimensions of root systems of four co-occurring fruit tree species from Botswana. Ann For Sci 57:463–475CrossRefGoogle Scholar
  32. Osmont KS, Sibout R, Hardtke CS (2007) Hidden branches: developments in root system architecture. Annu Rev Plant Biol 58:93–113CrossRefPubMedGoogle Scholar
  33. Ryser P (1998) Intra- and interspecific variation in root length, root turn-over and the underlying parameters. In: Lambers H, Poorter H, MMI VV (eds) Inherent variation in plant growth. Physiological mechanism and ecological consequences. Backhuys, Leiden, pp 441–465Google Scholar
  34. Ryser P, Lambers H (1995) Root and leaf attributes accounting for the performance of fast- and slow-growing grasses at different nutrient supply. Plant Soil 170:251–265CrossRefGoogle Scholar
  35. Sorgonà A, Cacco G (2002) Linking the physiological parameters of nitrate uptake with root morphology and topology in wheat (Triticum durum Desf.) and in citrus rootstock (Citrus volkameriana Ten & Pasq). Can J Bot 80:494–503CrossRefGoogle Scholar
  36. Sorgonà A, Abenavoli MR, Cacco G (2005) A comparative study between two citrus rootstocks: effect of nitrate on the root morpho-topology and net nitrate uptake. Plant Soil 270:257–267CrossRefGoogle Scholar
  37. Sorgonà A, Abenavoli MR, Gringeri PG, Cacco G (2007a) Comparing morphological plasticity of root orders in slow- and fast-growing citrus rootstocks supplied with different nitrate levels. Ann Bot 100:1287–1296CrossRefPubMedPubMedCentralGoogle Scholar
  38. Sorgonà A, Abenavoli MR, Gringeri PG, Lupini A, Cacco G (2007b) Root architecture plasticity of citrus rootstocks in response to nitrate availability. J Plant Nutr 30:1921–1932CrossRefGoogle Scholar
  39. Tatsumi J (2001) Fractal geometry of root system morphology: fractal dimension and multifractals. In: Proceedings 6th symposium international society root research, Nagoya, Japan, pp 24–25Google Scholar
  40. Tracy SR, Roberts JA, Black CR, McNeill A, Davidson R, Mooney SJ (2010) The X-factor: visualizing undisturbed root architecture in soils using X-ray computed tomography. J Exp Bot 61:311–313CrossRefPubMedGoogle Scholar
  41. Wahl S, Ryser P (2000) Root tissue structure is linked to ecological strategies of grasses. New Phytol 148:459–471CrossRefGoogle Scholar
  42. Walch-Liu P, Ivanov II, Filleur S, Gan Y, Remans T, Forde BG (2006) Nitrogen regulation of root branching. Ann Bot 97:875–881CrossRefPubMedPubMedCentralGoogle Scholar
  43. Walk TC, Van Erp E, Lynch JP (2004) Modelling applicability of fractal analysis to efficiency of soil exploration by roots. Ann Bot 94:119–128CrossRefPubMedPubMedCentralGoogle Scholar
  44. Wang H, Siopongco J, Wade LJ, Yamauchi A (2009) Fractal analysis on root systems of rice plants in response to drought stress. Environ Exp Bot 65:338–344CrossRefGoogle Scholar
  45. Zhou XC, Luo XW (2009) Advances in non-destructive measurement and 3D visualization methods for plant root based on machine vision. In: Proceedings of the 2nd international conference on biomedical engineering and informatics. Tianjin, BMEI’09, pp 1–5Google Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Antonio Lupini
    • 1
  • Fabrizio Araniti
    • 1
  • Antonio Mauceri
    • 1
  • Maria Princi
    • 1
  • Antonino Di Iorio
    • 2
  • Agostino Sorgonà
    • 1
  • Maria Rosa Abenavoli
    • 1
    Email author
  1. 1.Department AgrariaMediterranea UniversityReggio CalabriaItaly
  2. 2.Department of Biotechnology and Life ScienceUniversity of InsubriaVareseItaly

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