Visualization of Plant Microtubules

  • Elisa GrañaEmail author


Microtubules (MTs) are highly dynamic components of the cell cytoskeleton that are involved in many important processes such as cell division (chromosome movement, formation of preprophase band, phragmoplast, cortical band before preprophase, etc.), cellular transport (endocytosis, exocytosis, organelle movement: nuclei, chloroplasts, amyloplasts, etc.), and growth and differentiation (transport of cellulose precursors to the cell wall to form cellulose microfibrils, transition from division to expansion, stomata movement, etc.). Studying these cytoskeleton components is not only useful for understanding the mechanisms of cellular organization, but for understanding the response of cells to different stimuli that are known to change the microtubule array configuration. The knowledge of the microtubule dynamics opens the door to novel technical applications.



The implementation of these techniques was possible thanks to the invaluable assistance of Inés Pazos and Jesús Méndez from the Central Research Services (CACTI) of the University of Vigo.


  1. Alberts B (2008) Molecular biology of the cell, 5th edn. Garland Science, New YorkGoogle Scholar
  2. Araniti F, Graña E, Krasuska U, Bogatek R, Reigosa MJ, Abenavoli MR, Sánchez-Moreiras AM (2016) Loss of gravitropism in farnesene-treated Arabidopsis is due to microtubule malformations related to hormonal and ROS unbalance. PLoS One 11(8):e0160202CrossRefPubMedPubMedCentralGoogle Scholar
  3. Bartels PG, Hilton JL (1973) Comparison of trifularin, oryzalin, pronamide, prophan and colchicines treatments on microtubules. Pestic Biochem Physiol 3:462–472CrossRefGoogle Scholar
  4. Bhaskara GB, Wen T-N, Nguyen TT, Versules PE (2016) Protein phosphatase 2Cs and microtubule-associated stress protein 1 control microtubule stability, plant growth, and drought response. Plant Cell 29:169–191CrossRefPubMedPubMedCentralGoogle Scholar
  5. Celler K, Fujita M, Kawamura E, Ambrose C, Herburger K, Holzinger A, Wasteneys GO (2016) Microtubules in plant cells: strategies and methods for immunofluorescence, transmission electron microscopy and live cell imaging. In: Gavin RH (ed) Cytoskeleton: methods and protocols, methods in molecular biology, vol 1365. pp 155–184Google Scholar
  6. Chen X, Grandont L, Li H, Hauschild R, Paque S, Abuzeineh A, Rakusová H, Benkova E, Perrot-Rechenmann C, Friml J (2014) Inhibition of cell expansion by rapid ABP-1 mediated auxin effect of microtubules. Nature 516:90–93CrossRefPubMedPubMedCentralGoogle Scholar
  7. Collings DA, Wasteneys GO (2005) Actin microfilament and microtubule distribution in the expanding root of Arabidopsis thaliana. Can J Bot 83:579–590CrossRefGoogle Scholar
  8. Dayan FE, Hernandez A, Allen SN, Moraes RM, Vroman JA, Avery MA, Duke SO (1999) Comparative phytotoxicity of artemisin and several sesquiterpene analogues. Phytochemistry 50(607):614Google Scholar
  9. Dayan FE, Duke SO, Grossmann K (2010) Herbicides as probes in plant biology. Weed Sci 58(3):340–350CrossRefGoogle Scholar
  10. Donhauser ZJ, Jobs WB, Binka EC (2010) Mechanics of microtubules: effects of protofilament orientation. Biophys J 99(5):1668–1675CrossRefPubMedPubMedCentralGoogle Scholar
  11. Funada R (2008) Microtubules and the control of wood formation. In: Nick P (ed) Plant microtubules. Plant cell monographs, vol 11. Springer, Berlin, pp 83–119Google Scholar
  12. Gao Y, Valnberg IE, Chow RL, Cowan NJ (1993) Two cofactors and cytoplasmic chaperonin are required for the folding of α- and β-tubulin. Mol Cell Biol 13:2478–2485CrossRefPubMedPubMedCentralGoogle Scholar
  13. Goddard RH, Wick SM, Silflow CD, Snustad DP (1994) Microtubule components of the plant cell cytoskeleton. Plant Physiol 104:1–6CrossRefPubMedPubMedCentralGoogle Scholar
  14. Guo L, Ho CK, Kong Z, Lee YR, Qian Q, Liu B (2009) Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome. Ann Bot 103:387–402CrossRefPubMedGoogle Scholar
  15. Holzinger A, Wasteneys G, Lütz C (2007) Investigating cytoskeletal function in chloroplast protrusion formation in the arctic-alpine plant Oxyria digyna. Plant Biol 9(3):400–410CrossRefPubMedGoogle Scholar
  16. Holzinger A, Kawamura E, Wasteneys GO (2009) Strategies for imaging microtubules in plant cells. Methods Mol Biol 586:243–262CrossRefPubMedGoogle Scholar
  17. Hyams JS, Lloyd CW (1994) Microtubules. Wiley, New YorkGoogle Scholar
  18. Jordan A, Hadfield JA, Lawrence NJ, McGown AT (1998) Tubulin as a target for anticancer drugs: agents which interact with the mitotic spindle. Med Res Rev 18:259–296CrossRefPubMedGoogle Scholar
  19. Kwiatkowska D (2006) Flower primordium formation at the Arabidopsis shoot apex: quantitative analysis of surface geometry and growth. J Exp Bot 57:571–580CrossRefPubMedGoogle Scholar
  20. Landrein B, Hamant O (2013) How mechanical stress controls microtubule behavior and morphogenesis in plants: history, experiments and revisited theories. Plant J 75:324–338CrossRefPubMedGoogle Scholar
  21. Ledbetter MC, Porter KR (1963) A ‘microtubule’ in plant cell fine structure. J Cell Biol 19:239–250CrossRefPubMedPubMedCentralGoogle Scholar
  22. Lehnen LP Jr, Vaughn KC (1991) Immunofluorescence and electron microscopic investigations of the effects of ditiopyr on onion root tips. Pestic Biochem Physiol 40(1):58–67CrossRefGoogle Scholar
  23. Lehnen LP Jr, Vaughan MA, Vaughn KC (1990) Terbutol affects spindle microtubule organizing centers. J Exp Bot 41(5):537–546CrossRefGoogle Scholar
  24. Liu B, Joshi HC, Wilson TJ, Silflow CD, Palevitz BA, Snustad DP (1994) γ-tubulin in Arabidopsis: gene sequence, immunoblot, and immunofluorescence studies. Plant Cell 6:303–314CrossRefPubMedPubMedCentralGoogle Scholar
  25. Mao T, Jin L, Li H, Liu B, Yuan M (2005) Two microtubule-associated proteins of the Arabidopsis MAP65 family function differently on microtubules. Plant Physiol 138:654–662CrossRefPubMedPubMedCentralGoogle Scholar
  26. Marc J (1997) Microtubule-organizing center in plants. Trends Plant Sci 2(6):223–230CrossRefGoogle Scholar
  27. Mei Y, Gao HB, Yuan M, Xue HW (2012) The Arabidopsis ARCP protein, CSI1, which is required for microtubule stability, is necessary for root and anther development. Plant Cell 24:1066–1080CrossRefPubMedPubMedCentralGoogle Scholar
  28. Mitchison TJ, Kirschner MW (1984) Dynamic instability of microtubule growth. Nature 312:237–242CrossRefGoogle Scholar
  29. Nakamura M, Naoi K, Shoji T, Hashimoto T (2004) Low concentrations of propyzamide and oryzalin alter microtubule dynamics in Arabidopsis epidermal cells. Plant Cell Physiol 45(9):1330–1334CrossRefPubMedGoogle Scholar
  30. Nick P (2008a) Control of cell axis. In: Nick P (ed) Plant microtubules. Plant cell monographs, vol 11. Springer, Berlin, pp 3–46Google Scholar
  31. Nick P (2008b) Microtubules as sensors for abiotic stimuli. In: Nick P (ed) Plant microtubules. Plant cell monographs, vol 11. Springer, Berlin, pp 175–203Google Scholar
  32. Nick P (2013) Microtubules, signalling and abiotic stress. Plant J 75:309–323CrossRefPubMedGoogle Scholar
  33. Oliva A, Moraes RM, Watson SB, Duke SO, Dayan FE (2002) Aryltetralin lignans inhibit plant growth by affecting the formation of mitotic microtubular organizing centers. Pestic Biochem Physiol 72:45–54CrossRefGoogle Scholar
  34. Panteris E, Adamakis I-DS, Daras G, Hatzopoulos P, Rigas S (2013) Differential responsiveness of cortical microtubule orientation to suppression of cell expansion among the developmental zones of Arabidopsis thaliana root apex. PLoS One 8(12):e 82442CrossRefGoogle Scholar
  35. Reddy AS (2001) Molecular motors and their functions in plants. Int Rev Cytol 204:97–178CrossRefPubMedGoogle Scholar
  36. Senseman SA (2007) In: Senseman SA (ed) Herbicide handbook, 9th edn. Weed Science Society of America, LawrenceGoogle Scholar
  37. Vaughn KC (2006) The abnormal cell plates formed after microtubule disrupter herbicide treatment are enriched in callose. Pestic Biochem Physiol 84:63–71CrossRefGoogle Scholar
  38. Vaughn KC, Harper JDI (1998) Microtubule organizing centers and nucleating sites in land plants. Int Rev Cytol 181:75–149CrossRefPubMedGoogle Scholar
  39. Vaughn KC, Vaughan MA (1988) Mitotic disrupters from higher plants. In: Cutler HG (ed) Biologically active natural products: potential use in agriculture. ACS symposium series 380, Washington, DC. pp 273–293Google Scholar
  40. Vaughn KC, Marks MD, Weeks DP (1987) A dinitroaniline-resistant mutant of Eleusine indica exhibits cross-resistance and supersensitivity to antimicrotubule herbicides and drugs. Plant Physiol 83:956–964CrossRefPubMedPubMedCentralGoogle Scholar
  41. Wang SH, Kurepa J, Hashimoto T, Smalle JA (2011) Salt stress-induced disassembly of Arabidopsis cortical microtubule arrays involves 26S proteasome dependent degradation of SPIRAL1. Plant Cell 23:3412–3427CrossRefPubMedPubMedCentralGoogle Scholar
  42. Wasteneys GO (2004) Progress in understanding the role of microtubules in plant cells. Curr Opin Plant Biol 7(6):651–660CrossRefPubMedGoogle Scholar
  43. Yuan M, Shaw PJ, Warn RM, Lloyd CW (1994) Dynamic reorientation of cortical microtubules, from transverse to longitudinal, in living plant cells. Proc Natl Acad Sci U S A 91:6050–6053CrossRefPubMedPubMedCentralGoogle Scholar

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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Plant Ecophysiology Laboratory, Department of Plant Biology and Soil ScienceUniversity of VigoVigoSpain

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