Acquiring Data: Targets, Problems and Solutions

  • Graham E. Rotheray
Part of the Zoological Monographs book series (ZM, volume 4)


A priority for improving knowledge of the Cyclorrhapha is discovering poorly known and unknown larvae and extracting from them basic data. Basic data include those obtained from diagnosing morphology, finding taxonomic affinities and distinctions, specifying development sites and feeding modes, providing instructions for finding larvae in the field, i.e. fieldcraft, and giving collection locations of preserved larvae and reared adults. These data provide a foundation informing other subjects and more detailed assessments. Difficulties involved in developing fieldcraft, rearing and preserving larvae, extracting character data and making feeding tests to determine feeding mode are discussed. The information value of combining analyses of morphology with those of movement via observations and digital filming and correlating the results to development site variables as a means of investigating larval ecomorphology is considered. The complications of inherent and convergent similarity are also evaluated.


  1. Ball SG, Morris RKA (2014) A review of the scarce and threatened flies of Great Britain. Part 6: Syrphidae. Species Status 9:1–130. Joint Nature Conservation Committee, PeterboroughGoogle Scholar
  2. Basden EB (1954) The distribution and biology of Drosophilidae (Diptera) in Scotland, including a new species of Drosophila. Trans R Soc Edinb 62:603–654CrossRefGoogle Scholar
  3. Benton MJ, Emerson BC (2007) How did life become so diverse? The dynamics of diversification according to the fossil record and molecular phylogenetics. Palaeontology 50:23–40CrossRefGoogle Scholar
  4. Berrigan D, Leighton JRB (1993) Bioenergetic and kinematic consequences of limblessness in larval Diptera. J Exp Biol 179:245–259PubMedPubMedCentralGoogle Scholar
  5. Berrigan D, Pepin DJ (1995) How maggots move: allometry and kinematics of crawling in larval Diptera. J Insect Physiol 41:329–337CrossRefGoogle Scholar
  6. Bland KP, Horsfield D (2016) Distribution and biology of Palloptera scutellata (Macquart) (Diptera, Pallopteridae) in south-east Scotland. Dipt Digest (2nd Series) 23:51–53Google Scholar
  7. Borkent A, Brown BV, Adler PH, De Souza AD, Barber K, Bickel D, Boucher S, Brooks SE, Burger J, Burington ZL, Capellari RS, Costa DNR, Cumming JM, Curler G, Dick CW, Epler JH, Fisher E, Gaimari SD, Gelhaus J, Grimaldi DA, Hash J, Hauser M, Hippa H, Ibáñez-Bernal S, Jaschof M, Kameneva EP, Kerr PH, Korneyev V, Korytkowski CA, Kung G-A, Kvifte GM, Lonsdale O, Marshall SA, Mathis WN, Michelsen V, Naglis S, Norrbom AL, Paiero S, Pape T, Pereira-Colavite A, Pollet M, Rochefort S, Rung A, Runyon JB, Savage J, Silva VC, Sinclair BS, Skevington JH, Stireman JO, Swann J, Vilkamaa P, Wheeler T, Whitworth T, Wong M, Wood DM, Woodley N, Yau T, Zavortink TJ, Zumbado MA (2018) Remarkable fly (Diptera) diversity in a patch of Costa Rican cloud forest: why inventory is a vital science. Zootaxa 4402:53–90PubMedCrossRefPubMedCentralGoogle Scholar
  8. Brake I (1997) Ecological studies on Carnidae (Diptera) on the bird island of Mellum. Studia Dipterol 4:201–210Google Scholar
  9. Buck M (1997) Sphaeroceridae (Diptera) reared from various types of carrion and other decaying substrates in Southern Germany, including new faunistic data on some rarely collected species. Eur J Entomol 94:137–151Google Scholar
  10. Chandler PJ (2001) The flat-footed flies (Diptera: Opetiidae and Platypezidae) of Europe. Fauna Entomol Scand 36:1–276Google Scholar
  11. Chapman EG, Foote BA, Malukiewicz J, Hoeh WR (2006) Parallel evolution of larval morphology and habitat in the snail-killing fly genus Tetanocera. J Evol Biol 19:1459–1474PubMedCrossRefPubMedCentralGoogle Scholar
  12. Coe RL (1938) Rediscovery of Callicera yerburyi Verrall (Diptera: Syrphidae); its breeding habits, with a description of the larva. Entomologist 71:97–102Google Scholar
  13. Coe RL (1941) Callicera rufa Schummel (Diptera : Syrphidae); colour variation of abdominal hairs in the adult, with a note on longevity of the larva. Entomologist 74:131–132Google Scholar
  14. Cohen AC (2001) Formalizing insect rearing and artificial diet technology. Am Entomol 47:198–206CrossRefGoogle Scholar
  15. Collin JE (1933) Five new species of Diptera. Entomol Mon Mag 69:272–275Google Scholar
  16. Courtney GW, Sinclair BJ, Meier R (2000) Morphology and terminology of Diptera larvae. In: Papp L, Darvas B (eds) Contributions to a manual of Palaearctic Diptera, vol 1. Science Herald, Budapest, pp 85–161Google Scholar
  17. de Meijere JCH (1900) Ueber die larva von Lonchoptera. Ein Beitrag zur Kenntnis der cyclorraphen Dipterenlarven Zool Jahrb (Ab Syst) 14:87–132Google Scholar
  18. Deeming JC (1998) A description of the puparium of Gymnochiromyia inermis (Collin, 1933) from England (Diptera : Chyromyidae). Studia Dipterol 5:30–32Google Scholar
  19. Dempewolf M (2001) Larvalmorphologie und phylogenie der Agromyzidae (Diptera). Ph.D. thesis, University of Bielefeld, Bielefeld, GermanyGoogle Scholar
  20. Doczkal D (1996) Observations on host plants and behaviour of egg-laying females of Cheilosia Meigen (Diptera Syrphidae) in Central Europe. Volucella 2:77–85Google Scholar
  21. Dowding VM (1967) The function and ecological significance of the pharyngeal ridges occurring in the larvae of some cyclorrhaphous Diptera. Parasitol (Cam) 57:371–388CrossRefGoogle Scholar
  22. Dušek J, Láska P (1976) European species of Metasyrphus: key, descriptions and notes (Diptera, Syrphidae). Acta Entomol Bohemoslov 73:263–282Google Scholar
  23. Ferrar P (1979) The immature stages of dung-breeding muscoid flies in Australia, with notes on the species and keys to larvae and puparia. Aust J Zool Suppl Ser 27:1–106CrossRefGoogle Scholar
  24. Ferrar P (1987) A guide to the breeding habits and immature stages of Diptera Cyclorrhapha. Entomonograph 8:1–907Google Scholar
  25. Greve L (1993) Family Pallopteridae (Diptera) in Norway. Fauna Norv Ser B 40:37–44Google Scholar
  26. Günther MN, Nettesheim G, Shubeita GT (2016) Quantifying and predicting Drosophila larvae crawling phenotypes. Sci Rep 10:1–10. CrossRefGoogle Scholar
  27. Harrison DA, Cooper RL (2003) Characterization of development, behavior and neuromuscular physiology in the phorid fly, Megaselia scalaris. Comp Biochem Physiol A Mol Integr Physiol 136:427–439PubMedCrossRefPubMedCentralGoogle Scholar
  28. Hartley JC (1961) A taxonomic account of the larvae of some British Syrphidae. Proc Zool Soc Lond 136:505–573CrossRefGoogle Scholar
  29. Headrick DH, Goeden RD (1996) The biology of nonfrugivorous tephritid fruit flies. Annu Rev Entomol 43:217–241CrossRefGoogle Scholar
  30. Heckscher ES, Lockery SR, Doe CQ (2012) Characterization of Drosophila larval crawling at the level of organism, segment, and somatic body wall musculature. J Neurosci 32:12460–12471PubMedPubMedCentralCrossRefGoogle Scholar
  31. Hennig W (1976) Das Hypopygium von Lonchoptera lutea Panzer und die phylogenetischen Verwandtschaftsbeziehungen der Cyclorrhapha (Diptera). Stuttg Beitr Naturkd 283:1–63Google Scholar
  32. Keilin D (1912) Structure du pharynx en fonction du regime chez les larves des Diptères cyclorhaphes. C R Hebd Seanc Acad Sci Paris 155:1548–1550Google Scholar
  33. Keilin D (1917) Recherches sur les Anthomyides a larves carnivores. Parasitology (Cam) 9:325–450CrossRefGoogle Scholar
  34. Keilin D, Tate P (1930) On certain semi-carnivorous anthomyid larvae. Parasitol (Cam) 22:168–181CrossRefGoogle Scholar
  35. Lahuatte PF, Lincango MP, Heimpel GE, Causton CE (2016) Rearing larvae of the avian nest parasite, Philornis downsi (Diptera: Muscidae), on chicken blood-based diets. J Insect Sci 16:1–7CrossRefGoogle Scholar
  36. Lewis T, Taylor LR (1965) Diurnal periodicity of flight by insects. Trans R Ent Soc Lond 116:393–435CrossRefGoogle Scholar
  37. Lonsdale O, Marshall S (2011) Clusiidae. Version 06 Jan 2011. In: The Tree of Life Web Project.
  38. MacGowan I, Rotheray GE (2006) Callicera rufa (Diptera, Syrphidae) status and trends: an update. Dipt Digest 13:113–118Google Scholar
  39. MacGowan I, Rotheray GE (2008) British Lonchaeidae (Diptera, Cyclorrhapha, Acalyptratae). Handbk Ident Br Insects 10:1–142Google Scholar
  40. Maibach A, de Tiefenau G (1992) Staining technique for the integument of dead and living aquatic larvae (Diptera: Syrphidae). Entomol Gener 17:69–71CrossRefGoogle Scholar
  41. Marcos-García MÁ, García-López A, Zumbado MA, Rotheray GE (2012) Sampling methods for assessing syrphid biodiversity (Diptera: Syrphidae) in tropical forests. Environ Entomol 41:1544–1552PubMedCrossRefPubMedCentralGoogle Scholar
  42. Martinek V (1977) Species of Genus Palloptera Fallén, 1820 (Dipt., Pallopteridae) in Czechoslovakia. Stud Entomol Forestalia 12:203–220Google Scholar
  43. McAlpine JF, Munroe DD (1968) Swarming of lonchaeid flies and other insects, with descriptions of four new species of Lonchaeidae (Diptera). Can Entomol 100:1154–1178CrossRefGoogle Scholar
  44. Meier R (1995) Cladistic analysis of the Sepsidae (Cyclorrhapha: Diptera) based on a comparative scanning electron microscopic study of larvae. Syst Entomol 20:99–128CrossRefGoogle Scholar
  45. Meier R, Lim GS (2009) Conflict, convergent evolution, and the relative importance of immature and adult characters in endopterygote phylogenetics. Annu Rev Entomol 54:85–104PubMedCrossRefPubMedCentralGoogle Scholar
  46. Morge G (1956) Ber Morphologie und lebensweise der bisher unbekannten Larven von Palloptera usta Meigen, Palloptera ustulata Fallén und Stegana coleoptrata Scopoli (Diptera). Beitr Ent 6:124–137Google Scholar
  47. Nartshuk EP, Andersson H (2012) The Frit Flies (Chloropidae, Diptera) of Fennoscandia and Denmark. Fauna Ent Scand 43:1–282CrossRefGoogle Scholar
  48. Nye IWB (1958) The external morphology of dipterous larvae occurring in the Gramineae of Britain. Trans R Ent Soc Lond 110:411–487CrossRefGoogle Scholar
  49. Papp L (1994) Morphology of third instar larva and puparium of three heleomyzid species (Diptera: Heleomyzidae). Acta Zool Acad Sci Hung 40:219–229Google Scholar
  50. Parra JR (2012) The evolution of artificial diets and their interactions in science and technology. In: Panizzi AR, Parra JR (eds) Insect bioecology and nutrition for integrated pest management. CRC Press, London, pp 51–92CrossRefGoogle Scholar
  51. Pérez-Lachaud G, Jahyny BJB, Ståhls G, Rotheray G, Delabie JHC, Lachaud J-P (2017) Rediscovery and reclassification of the dipteran taxon Nothomicrodon Wheeler, an exclusive endoparasitoid of gyne ant larvae. Sci Rep 7:45530. CrossRefPubMedPubMedCentralGoogle Scholar
  52. Perry I, Rotheray GE (2010) Dead wood and sap runs. In: Chandler PJ (ed) A Dipterist’s handbook, vol 15, 2nd edn. Amateur Entomologist’s Society, pp 144–157Google Scholar
  53. Roberts MJ (1971) The structure of the mouthparts of some calypterate dipteran larvae in relation to their feeding habits. Acta Zool 52:171–188CrossRefGoogle Scholar
  54. Roháček J, Barber KN (2011) Quametopia, a new genus of Nearctic Anthomyzidae (Diptera), with description of two new species, immature stages and life history. Eur J Entomol 108:287–326CrossRefGoogle Scholar
  55. Rotheray GE (1986) Colour, shape and defence in aphidophagous syrphid larvae (Diptera). Zool J Linnean Soc 88:201–216CrossRefGoogle Scholar
  56. Rotheray GE (1989) E. B. Basden’s collection of Diptera from bird and mammal nests, and mammal runs, burrows and droppings. Entomol Mon Mag 125:5–8Google Scholar
  57. Rotheray GE (1991) Larval stages of 17 rare and poorly known British hoverflies (Diptera: Syrphidae). J Nat Hist 25:945–969CrossRefGoogle Scholar
  58. Rotheray GE (1993) Colour guide to hoverfly larvae (Diptera, Syrphidae). Dipt Digest 9:1–156Google Scholar
  59. Rotheray GE (2011) The puparia of Chyromya femorellum (Chyromyidae), Camilla atrimana and Camilla fuscipes (Camillidae) (Diptera) reared from bird and mammal nests and burrows. Dipt Digest 18:81–93Google Scholar
  60. Rotheray GE (2012) Morphology of the puparium and breeding sites of eight species of Heleomyzidae (Diptera). J Nat Hist 46:2075–2102CrossRefGoogle Scholar
  61. Rotheray GE (2014) Development sites, feeding modes and early stages of seven European Palloptera species (Diptera, Pallopteridae). Zootaxa 3900:50–76PubMedCrossRefGoogle Scholar
  62. Rotheray GE (2016) Improving knowledge of the cyclorrhaphan larva (Diptera). J Nat Hist 50:2169–2198CrossRefGoogle Scholar
  63. Rotheray GE, Gilbert F (1999) Phylogeny of palaearctic Syrphidae (Diptera): evidence from larval stages. Zool J Linnean Soc 127:1–112CrossRefGoogle Scholar
  64. Rotheray GE, Gilbert F (2008) Phylogenetic relationships and the larval head of the lower Cyclorrhapha (Diptera). Zool J Linnean Soc 153:287–323CrossRefGoogle Scholar
  65. Rotheray GE, Gilbert F (2011) The natural history of hoverflies. Forrest Text, CardiganGoogle Scholar
  66. Rotheray GE, Hancock EG (2012) Puparial morphology and development sites of Neottiophilum praeustum, Allopiophila vulgaria, Mycetaulus bipunctatus and Prochyliza varipes (Diptera, Piophilidae). Dipt Digest 19:107–124Google Scholar
  67. Rotheray GE, Hewitt (2015) Development site, feeding mode and early stages of Palloptera scutellata (Macquart) (Diptera, Pallopteridae). Dipt Digest 22:157–170Google Scholar
  68. Rotheray GE, Horsfield D (2013) Development sites and early stages of eleven species of Clusiidae (Diptera) occurring in Europe. Zootaxa 3619:401–427PubMedCrossRefGoogle Scholar
  69. Rotheray GE, Lyszkowski R (2015) Diverse mechanisms of feeding and movement in Cyclorrhaphan larvae (Diptera). J Nat Hist 49:2139–2211CrossRefGoogle Scholar
  70. Rotheray GE, MacGowan I (1990) Re-evaluation of the status of Callicera rufa Schummel (Diptera: Syrphidae) in the British Isles. Entomologist 109:35–42Google Scholar
  71. Rotheray GE, MacGowan I (2000) Status and breeding sites of three presumed endangered Scottish saproxylic syrphids (Diptera, Syrphidae). J Insect Conserv 4:215–223CrossRefGoogle Scholar
  72. Rotheray GE, Wilkinson G (2015) Trophic structure and function in the larva of predatory muscid flies (Diptera, Muscidae). Zoomorphology 134:553–563CrossRefGoogle Scholar
  73. Rotheray GE, Hancock EG, Hewitt S, Horsfield D, MacGowan I, Robertson D, Watt K (2001) The biodiversity and conservation of saproxylic Diptera in Scotland. J Insect Conserv 5:77–85CrossRefGoogle Scholar
  74. Rotheray GE, Chandler PJ, Gilbert F (2004) Final stage larvae and puparia of Platypezidae (Diptera). Insect Syst Evol 35:79–105CrossRefGoogle Scholar
  75. Rotheray GE, Hancock EG, Marcos-Garcia M (2007) Neotropical Copestylum (Diptera, Syrphidae) breeding in bromeliads (Bromeliaceae) including 22 new species. Zool J Linnean Soc 150:267–317CrossRefGoogle Scholar
  76. Schluter D (2000) The ecology of adaptive radiation. Oxford University Press, OxfordGoogle Scholar
  77. Schmid U (1999) Die Larve von Cheilosia impressa (Diptera, Syrphidae). Volucella 4:113–119Google Scholar
  78. Semelbauer M, Kozánek M (2012) Morphology of preimaginal stages of Lauxania and Calliopum (Diptera: Lauxaniidae). Zootaxa 3346:1–28CrossRefGoogle Scholar
  79. Sewell BA, Atwood HL, Renger JJ, Wang J, Wu CF (1975) Improved stability of Drosophila larval neuromuscular preparation in haemolymph-like physiological solutions. J Comp Physiol A 175:179–191Google Scholar
  80. Singh P (1977) Artificial diets for Insects, mites and Spiders. IFI/Plenum Data Company, New YorkCrossRefGoogle Scholar
  81. Singh P, Moore RF (eds) (1985) Handbook of insect rearing, vol 1–2. Elsevier Science, AmsterdamGoogle Scholar
  82. Skidmore P (1966) The biology of Scoliocentra villosa (Meigen) (Dipt., Heleomyzidae). Entomol Mon Mag 102:94–98Google Scholar
  83. Skidmore P (1985) The biology of the Muscidae of the world. Junk, DordrechtGoogle Scholar
  84. Smith KVG (1989) An introduction to the immature stages of British flies. Handbk Ident Br Insects 10:1–280Google Scholar
  85. Ståhls G, Kahanpää J (2006) New data on Platypezidae and Opetiidae (Diptera) of Finland. Sahlbergi 11:1–6Google Scholar
  86. Tinkeu LN, Hance T (1998) Functional morphology of the mandibles of the larvae of Episyrphus balteatus (De Geer, 1776) (Diptera : Syrphidae). Int J Insect Morphol Embryol 27:135–142CrossRefGoogle Scholar
  87. Trautwein MD, Wiegmann BM, Beutel R, Kjer KM, Yeates DK (2012) Advances in insect phylogeny at the dawn of the postgenomic era. Annu Rev Entomol 57:449–468PubMedCrossRefGoogle Scholar
  88. Wheeler WM (1924) Two extraordinary larval myrmecophiles from Panama. Proc Natl Acad Sci U S A 10:237–244PubMedPubMedCentralCrossRefGoogle Scholar
  89. Wheeler QD (2008) Undisciplined thinking: morphology and Hennig’s unfinished revolution. Syst Entomol 33:2–7CrossRefGoogle Scholar
  90. Wiegmann BM, Trautwein MD, Winkler IS, Barra NB, Kima J-W, Lambkin C, Berton MA, Cassela BK, Bayless KM, Heimberg AM, Wheeler BM, Petersone KJ, Pape T, Sinclair BJ, Skevington JH, Blagoderov V, Caravask J, Narayanan Kutty SN, Schmidt-Ott U, Kampmeier GE, Thompson FC, Grimaldi DA, Beckenbach AT, Courtney GM, Friedrich M, Meier R, Yeates DK (2011) Episodic radiations in the fly tree of life. Proc Natl Acad Sci U S A 108:5690–5695PubMedPubMedCentralCrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Graham E. Rotheray
    • 1
  1. 1.National Museums of ScotlandEdinburghUK

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