Current Trend in Cell Membrane Manipulation by Ultrasound and Underwater Shock Wave

  • S. F. Moosavi-Nejad
  • H. HosseiniEmail author
Conference paper


The paper presents our recent findings in reparable cell membrane manipulation by ultrasound and underwater shock wave. We introduced innovative in vitro methods including free suspension and capillary micro-gripping systems equipped with micro transducers to achieve the maximum level of experimental flexibility for capturing real-time highly magnified images of cell-microbubble interactions. Insonation of single cells and microbubbles parallel with high-speed microphotography and fluorescence microscopy allowed us to identify dynamic responses of cell membrane to microbubble streaming dynamics in correlation with sonoporation. Our results showed that bubble oscillation in close contact with the cell membrane can cause local deformation and transient porosity in the cell membrane with minimum cell toxicity.


  1. 1.
    S. Moosavi-Nejad, S.H.R. Hosseini, H. Akiyama, K. Tachibana, Reparable cell sonoporation in suspension: Theranostic potential of microbubble. Theranostics 6(4), 446–455 (2016)CrossRefGoogle Scholar
  2. 2.
    S. Moosavi-Nejad, S.H.R. Hosseini, H. Akiyama, K. Tachibana, Optical observation of cell sonoporation with low intensity ultrasound. Biochem. Biophys. Res. Commun. 413, 218–223 (2011)CrossRefGoogle Scholar
  3. 3.
    S. Moosavi-Nejad, S.H.R. Hosseini, Current trends in bioelectrics for reversible cell membrane manipulation, comment on “physical methods for genetic transformation of fungi and yeast”. Phys. Life Rev. 11, 212 (2014)CrossRefGoogle Scholar
  4. 4.
    V. Menezes, H. Hosseini, S. Moosavi-Nejad, K.J. Irimpan, H. Akiyama, Motion of free-surface of shock-compressed water on emergence of rarefaction. Appl. Phys. Lett. 107, 143701 (2015)CrossRefGoogle Scholar
  5. 5.
    H. Hosseini, S. Moosavi-Nejad, H. Akiyama, V. Menezes, Shock wave interaction with interfaces between materials having different acoustic impedances. Appl. Phys. Lett. 104, 103701 (2014)CrossRefGoogle Scholar
  6. 6.
    S. Moosavi-Nejad, H. Takahashi, H. Hosseini, A. Watanabe, H. Endo, K. Narihira, T. Kikuta, K. Tachibana, Acute effects of sono-activated photocatalytic titanium dioxide nanoparticles on oral squamous cell carcinoma. Ultrason. Sonochem. 32, 95–101 (2016)CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Bioelectrics DepartmentInstitute of Pulsed Power Science, Kumamoto UniversityKumamotoJapan
  2. 2.Bioelectrics DepartmentInstitute of Pulsed Power Science & Graduate School of Science and Technology, Kumamoto UniversityKumamotoJapan

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