Advertisement

Genetic Variation of Wild and Hatchery Populations of Climbing Perch, Anabas testudineus (Bloch, 1792), in Peninsular Malaysia

  • Mohamed Ahmad Azfar 
  • Abdurahman Siti Waznah 
  • Akbar John
Chapter

Abstract

In order to increase the production of Anabas testudineus, selective breeding can be implemented to produce better performing stock. To initiate a successful selective breeding program, genetic variation is very important. This study was aimed to aid selective breeding of A. testudineus in Malaysia. Cytochrome b gene was selected as a molecular marker to investigate the current status of genetic variation in hatchery and wild populations of A. testudineus. Based on cytochrome b analysis, all existing hatchery populations in Malaysia belonged to a single haplotype. In contrast, five distinct haplotypes were discovered based on genotypic data of wild A. testudineus populations in Malaysia. Overall, this study recommended to introduce new genetic material from wild population to the synthetic base population to increase the genetic variation of the populations of A. testudineus. All of the information gathered is crucial to develop a sustainable selective breeding for A. testudineus and to improve quality and production of this species.

Keywords

Anabas testudineus Genetic Hatchery Cytochrome b Climbing perch 

Notes

Acknowledgment

This research was conducted with joint funding from the Ministry of Education (MOE), under Fundamental Research Grant Scheme (FRGS) vote 59229 and also from IIUM funding (RIGS 16-100-0264).

References

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215(3):403–410CrossRefGoogle Scholar
  2. Ambak MA, Isa MM, Zakaria MZ, Ghaffar MA (2010) Fishes of Malaysia. Penerbit Universiti Malaysia TerengganuGoogle Scholar
  3. Bungas K, Arfiati D, Marsoedi, Halim H (2013) Effects of protein levels on the growth of climbing perch, Anabas testudineus Galam type, in Peat Water. Int Res J Biol Sci 2(4):55–58Google Scholar
  4. Carr SM, Marshall HD (1991) Detection of intraspecific. DNA sequence variation in the mitochondrial cytochrome b gene of Atlantic Cod (Gadus morhua) by the polymerase chain reaction. Can J Fish Aquat Sci 48(1):48–52CrossRefGoogle Scholar
  5. Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.5): an integrated software package for population genetics data analysis. Evol Bioinforma 1:47–50CrossRefGoogle Scholar
  6. Filgueira R, Grant J, Strand Ø, Asplinc L, Aurec J (2010) A simulation model of carrying capacity for mussel culture in a Norwegian fjord: role of induced upwelling. Aquaculture 308(1–2):20–27CrossRefGoogle Scholar
  7. Gjedrem T, Robinson N, Rye M (2012) The importance of selective breeding in aquaculture to meet future demands for animal protein: a review. Aquaculture 350:117–129CrossRefGoogle Scholar
  8. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  9. Hebert PD, Cywinska A, Ball SL (2003) Biological identifications through DNA barcodes. Proc R Soc Lond B Biol Sci 270(1512):313–321CrossRefGoogle Scholar
  10. Hidayat S, Senanan W (2010) PCR-RFLP analysis of mitochondrial DNA to differentiate populations of Climbing Perch (Anabas testudineus) in Thailand. Burapha Sci J 15(2):87–98Google Scholar
  11. Iguchi K i, Watanabe K, Nishida M (1999) Reduced mitochondrial DNA variation in hatchery populations of ayu (Plecoglossus altivelis) cultured for multiple generations. Aquaculture 178(3–4):235–243CrossRefGoogle Scholar
  12. Lind C, Ponzoni R, Nguyen N, Khaw H (2012) Selective breeding in fish and conservation of genetic resources for aquaculture. Reprod Domest Anim 47(4):255–263CrossRefPubMedGoogle Scholar
  13. Lu X, Wang H, Liu B, Xiang J (2011) An effective method for parentage determination of the clam (Meretrix meretrix) based on SSR and COI markers. Aquaculture 318(1):223–228CrossRefGoogle Scholar
  14. McCarthy C (1997) Chromas v1. 4 computer package. Griffith University, AustraliaGoogle Scholar
  15. Perez-Enriquez R, Takagi M, Taniguchi N (1999) Genetic variability and pedigree tracing of a hatchery-reared stock of red sea bream (Pagrus major) used for stock enhancement, based on microsatellite DNA markers. Aquaculture 173(1–4):413–423CrossRefGoogle Scholar
  16. Rahman S, Monir MS, Khan MH (2013) Culture potentials of Climbing Perch, Thai Koi, Anabas Testudineus (Bloch) under different stocking densities in Northern Regions of Bangladesh. J Exp Biol Agric Sci 1(3):202–208Google Scholar
  17. Rozas J, Sánchez-DelBarrio JC, Messeguer X, Rozas R (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19(18):2496–2497CrossRefPubMedGoogle Scholar
  18. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, vol 2. Cold Spring Harbor Laboratory Press, New York, pp 14–19Google Scholar
  19. Sekino M, Saitoh K, Yamada T, Kumagai A, Hara M, Yamashita Y (2003) Microsatellite-based pedigree tracing in a Japanese flounder Paralichthys olivaceus hatchery strain: implications for hatchery management related to stock enhancement program. Aquaculture 221(1-4):255–263CrossRefGoogle Scholar
  20. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24(8):1596–1599CrossRefPubMedGoogle Scholar
  21. Thompson JD (1991) Phenotypic plasticity as a component of evolutionary change. Trends Ecol Evol 6(8):246–249CrossRefPubMedGoogle Scholar
  22. Tingga RCT, Abdullah MT (2011) Phylogeny and phylogeography of Aethalops from Sundaland using mitochondrial 12S rRNA gene. Pertanika J Trop Agric Sci 35(3):485–510Google Scholar
  23. Zalina I, Saad C, Christianus A, Harmin S (2012) Induced breeding and embryonic development of Climbing Perch (Anabas testudineus, Bloch). J Fish Aquat Sci 7(5):291–306CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Mohamed Ahmad Azfar 
    • 1
    • 2
  • Abdurahman Siti Waznah 
    • 2
  • Akbar John
    • 1
  1. 1.INOCEM Research Station, Kulliyyah of ScienceInternational Islamic University MalaysiaKuantanMalaysia
  2. 2.Department of Marine Science, Kulliyyah of ScienceInternational Islamic University MalaysiaKuantanMalaysia

Personalised recommendations