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Phylogenetic Diversity of Culturable Marine Actinobacteria Isolated from the Havelock Island, the Andamans, India

  • Gobalakrishnan Rajagopal
  • Sivakumar Kannan
Chapter

Abstract

Due to the excellent track record of actinobacteria and their potential, more attention is being paid to the isolation of novel actinobacteria for the diverse range of screening programs. In this context, present study was aimed to investigate the phylogenetic diversity of culturable marine actinobacteria isolated from the coast of Havelock Island, the Andamans, India. Actinobacterial colonies were isolated from the sediments of the six stations of the Havelock Island, using different media, viz., Kuster’s agar, Starch Casein agar, Actinomycetes isolation agar, Starch Casein nitrate agar, and AV agar. Higher actinobacterial population density was recorded in Kuster’s agar. A total of 19 morphologically distinct actinobacterial strains were subjected to identification using cultural, cell wall chemotypical, morphological, physiological, and molecular (16S rDNA) characteristics; 18 out of 19 isolates belonged to Streptomyces, and 1 isolate belonged to Actinoalloteichus. This study has led us to conclude that the coastal sediments of the Havelock Island of the Andamans are good source for obtaining culturable marine actinobacteria. Isolation of such diverse marine actinobacteria from the marine sediments could yield novel candidates, a valuable source for various scientific and biotechnological applications and genes for their biosynthesis. Further research including molecular characterizations of the isolated strains are important to pursue their biotechnological importance and ecological roles.

Keywords

Actinobacteria Havelock Island Cultivable diversity Streptomyces Actinoalloteichus 

Notes

Acknowledgments

The authors thank the Dean and Faculty of Marine Sciences and the authorities of Annamalai University, and one of the authors (R.G.) thank the Principal and the authorities of J.J. College of Arts and Science, for providing with necessary facilities. They also thank Prof. L. Kannan, Former Vice Chancellor, Thiruvalluvar University, Vellore, for encouragement and editorial support.

Conflict of Interest

We declare that we have no conflict of interest.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Gobalakrishnan Rajagopal
    • 1
  • Sivakumar Kannan
    • 2
  1. 1.Department of BiotechnologyJ.J. College of Arts and Science (Autonomous)PudukkottaiIndia
  2. 2.Faculty of Marine Sciences, Centre of Advanced Study in Marine BiologyAnnamalai UniversityParangipettaiIndia

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