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Metallomics pp 245-270 | Cite as

New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells

  • Javier Jiménez-Lamana
  • Joanna Szpunar
  • Ryszard Łobinski
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1055)

Abstract

Single cells represent the basic building units of life, and thus their study is one the most important areas of research. However, classical analysis of biological cells eludes the investigation of cell-to-cell differences to obtain information about the intracellular distribution since it only provides information by averaging over a huge number of cells. For this reason, chemical analysis of single cells is an expanding area of research nowadays. In this context, metallomics research is going down to the single-cell level, where high-resolution high-sensitive analytical techniques are required. In this chapter, we present the latest developments and applications in the fields of single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS), mass cytometry, laser ablation (LA)-ICP-MS, nanoscale secondary ion mass spectrometry (nanoSIMS), and synchrotron X-ray fluorescence microscopy (SXRF) for single-cell analysis. Moreover, the capabilities and limitations of the current analytical techniques to unravel single-cell metabolomics as well as future perspectives in this field will be discussed.

Keywords

Single-cell analysis ICP-MS Laser ablation NanoSIMS X-ray fluorescence Imaging Intracellular distribution Metal content 

Abbreviations

ALOD

Absolute limit of detection

AP-MALDI

Atmospheric pressure matrix-assisted laser desorption ionization

CE

Capillary electrophoresis

cryo-XT

Cryo nanoscale X-ray tomography

CyTOF

Cytometry by time-of-flight

ESI-MS

Electrospray ionization mass spectrometry

fs-LI-O-TOFMS

Femtosecond laser ionization orthogonal time-of-flight mass spectrometry

HECIS

High-efficiency cell introduction system

HPCN

High performance concentric nebulizer

ICP-AES

Inductively coupled plasma atomic emission spectrometry

ICP-MS

Inductively coupled plasma mass spectrometry

ICP-SFMS

Inductively coupled plasma sector field mass spectrometry

ICP-TOFMS

Inductively coupled plasma time-of-flight mass spectrometry

LADE

Liquid-assisted droplet ejection

LA-ICP-MS

Laser ablation inductively coupled plasma mass spectrometry

LB-HPCN

Large-bore high performance concentric nebulizer

MALDI

Matrix-assisted laser desorption ionization

μDG

Micro-droplet generator

M-DIS

Micro-droplet injection system

μFI

Micro-flow Injection

μXRF

Micro-X-ray fluorescence

NPs

Nanoparticles

PBS

Phosphate-buffered saline

PDMS

Poly(dimethylsiloxane)

PFH

Perfluorohexane

QDs

Quantum dots

RR

Ruthenium red

SC-ICP-MS

Single cell inductively coupled plasma mass spectrometry

SIMS

Secondary ion mass spectrometry

SNMS

Secondary neutral mass spectrometry

SOD

Superoxide dismutase

SP-ICP-MS

Single particle inductively coupled plasma mass spectrometry

SR-nXRF

Synchrotron radiation nano-X-ray fluorescence

SXRF

Synchrotron X-ray fluorescence

TEM

Transmission electron microscopy

TOF-SIMS

Time-of-flight secondary ion mass spectrometry

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Javier Jiménez-Lamana
    • 1
  • Joanna Szpunar
    • 1
  • Ryszard Łobinski
    • 1
  1. 1.Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials (IPREM)UMR 5254, CNRS-UPPAPauFrance

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