Toward the Optimization of a Plant-Based Oral Vaccine Against Cysticercosis

  • Edda Sciutto
  • Marisela Hernández
  • Jacquelynne Cervantes-Torres
  • Elizabeth Monreal-Escalante
  • Omayra Bolaños-Martínez
  • Juan Francisco Rodríguez
  • Gladis Fragoso
  • Sergio Rosales-MendozaEmail author


It is recognized that an effective, low-cost oral vaccine may effectively contribute to prevent Taenia solium cysticercosis; plant-based vaccines, on the other hand, can make this goal feasible. Plants are optimal platforms for the massive production of oral vaccines. In this chapter, advances toward the development of oral plant-based vaccine against cysticercosis are reviewed.


Oral vaccine Transgenic plant Transplastomic plant Carica papaya Daucus carota Nicotiana tabacum Taenia solium 


  1. Assana E, Kyngdon CT, Gauci CG et al (2010) Elimination of Taenia solium transmission to pigs in a field trial of the TSOL18 vaccine in Cameroon. Int J Parasitol 40:515–519. Scholar
  2. Bano I, Sajjad H, Shah AM et al (2017) A review of rabies disease, its transmission and treatment. J Anim Health Prod 4:140–144CrossRefGoogle Scholar
  3. Betancourt MA, de Aluja AS, Sciutto E et al (2012) Effective protection induced by three different versions of the porcine S3Pvac anticysticercosis vaccine against rabbit experimental Taenia pisiformis cysticercosis. Vaccine 30:2760–2767. Scholar
  4. Chambers MA, Graham SP, La Ragione RM (2016) Challenges in veterinary vaccine development and immunization. Chapter 1. Vaccine design: methods and protocols. Edited by Sunil Thomas. Human Press, USACrossRefGoogle Scholar
  5. Cox E, Melkebeek V, Devriendt B et al (2014) Vaccines against enteric E. coli infections in animals. In: Pathogenic Escherichia coli: molecular and cellular microbiology. Caister Academic Press, pp 255–270Google Scholar
  6. De Aluja AS (2008) Cysticercosis in the pig. Curr Top Med Chem 8:368–374CrossRefPubMedGoogle Scholar
  7. Fleury A, Sciutto E, Larralde C (2012) Neurocysticercosis is still prevalent in Mexico. Salud Publica Mex 54:632–636CrossRefPubMedGoogle Scholar
  8. Fleury A, Sciutto E, de Aluja AS et al (2013) Control of Taenia solium transmission of taeniosis and cysticercosis in endemic countries: the roles of continental networks of specialists and of local health authorities. In: Fleury A (ed) Novel aspects on cysticercosis and neurocysticercosis. InTech, AustriaGoogle Scholar
  9. Fragoso G, Hernández M, Cervantes-Torres J et al (2017) Transgenic papaya: a useful platform for oral vaccines. Planta 245:1037–1048. Scholar
  10. Hernández M, Cabrera-Ponce JL, Fragoso G et al (2007) A new highly effective anticysticercosis vaccine expressed in transgenic papaya. Vaccine 25:4252–4260CrossRefPubMedGoogle Scholar
  11. Hirlekar R, Bhairy S (2017) Edible vaccines: an advancement in oral immunization. Asian J Pharm Clin Res 10:82–88Google Scholar
  12. Huerta M, De Aluja AS, Fragoso G et al (2001) Synthetic peptide vaccine against Taenia solium pig cysticercosis: successful vaccination in a controlled field trial in rural Mexico. Vaccine 20:262–266CrossRefPubMedGoogle Scholar
  13. Kesik-Brodacka M, Lipiec A, Kozak Ljunggren M et al (2017) Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae. PLoS Negl Trop Dis 11:e0005451. Scholar
  14. Kim MY, Kim BY, Oh SM et al (2016) Oral immunisation of mice with transgenic rice calli expressing cholera toxin B subunit fused to consensus dengue cEDIII antigen induces antibodies to all four dengue serotypes. Plant Mol Biol 92:347–356. Scholar
  15. Lightowlers MW, Donadeu M, Elaiyaraja M et al (2016) Anamnestic responses in pigs to the Taenia solium TSOL18 vaccine and implications for control strategies. Parasitology 143:416–420CrossRefPubMedGoogle Scholar
  16. Liu BS, Liu XY, Qian C (2007) An efficient tool for the construction of multiple-cistronic vectors: FMDV 2A. Sheng Wu Gong Cheng Xue Bao 23:765–769PubMedGoogle Scholar
  17. Lizuka M, Wakasa Y, Tsuboi H et al (2014) Prophylactic effect of the oral administration of transgenic rice seeds containing altered peptide ligands of type II collagen on rheumatoid arthritis. Biosci Biotechnol Biochem 78:1662–1668. Scholar
  18. Lugade AA, Kalathil S, Heald JL et al (2010) Transgenic plant-based oral vaccines. Immunol Invest 39:468–482CrossRefPubMedGoogle Scholar
  19. Marcin Sierra M, Arroyo M, Cadena Torres M et al (2017) Extraparenchymal neurocysticercosis: demographic, clinicoradiological, and inflammatory features. PLoS Negl Trop Dis 9:e0005646CrossRefGoogle Scholar
  20. Mason HS, Haq TA, Clements JD et al (1998) Edible vaccine protects mice against Escherichia coli heatlabile enterotoxin (LT): potatoes expressing a synthetic LT-B gene. Vaccine 16:1336–1343CrossRefPubMedGoogle Scholar
  21. Meeusen Els NT, Walker J, Peters A et al (2007) Current status of veterinary vaccines. Clin Microbiol Rev 20:489–510CrossRefPubMedPubMedCentralGoogle Scholar
  22. Minskaia E, Nicholson J, Ryan MD (2013) Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications. BMC Biotechnol 13:67. Scholar
  23. Minskaia E, Ryan MD (2013) Protein coexpression using FMDV 2A: effect of “linker” residues. Biomed Res Int 291730.
  24. Monreal-Escalante E, Bañuelos-Hernández B, Hernández M et al (2015) Expression of multiple Taenia solium Immunogens in plant cells through a ribosomal skip mechanism. Mol Biotechnol 57:635–643CrossRefPubMedGoogle Scholar
  25. Monreal-Escalante E, Govea-Alonso DO, Hernández M et al (2016) Towards the development of an oral vaccine against porcine cysticercosis: expression of the protective TSOL18/HP6-TSOL antigen in transgenic carrots cells. Planta 243:675–685CrossRefPubMedGoogle Scholar
  26. Morales J, Martínez JJ, Manoutcharian K et al (2008) Inexpensive anti-cysticercosis vaccine: S3Pvac expressed in heat inactivated M13 filamentous phage proves effective against naturally acquired Taenia solium porcine cysticercosis. Vaccine 26:2899–2905. Scholar
  27. Muir WI, Bryden WL, Husband AJ (2000) Immunity, vaccination and the avian intestinal tract. Dev Comp Immunol 24:325–342CrossRefPubMedGoogle Scholar
  28. Mutoloki S, Munang’andu HM, Evensen Ø (2015) Oral vaccination of fish-antigen preparations, uptake, and immune induction. Front Immunol 6:519. Scholar
  29. Park S, Lee JB, Kim KJ et al (2013) Efficacy of a commercial live attenuated Lawsonia intracellularis vaccine in a large scale field trial in Korea. Clin Exp Vaccine Res 2:135–139CrossRefPubMedPubMedCentralGoogle Scholar
  30. Parkhouse RM, Bonay P, González LM et al (2008) TSOL18/HP6-Tsol, an immunogenic Taenia solium oncospheral adhesion protein and potential protective antigen. Parasitol Res 102:921–926CrossRefPubMedGoogle Scholar
  31. Rosales-Mendoza S, Govea-Alonso DO, Monreal-Escalante E et al (2012) Developing plant-based vaccines against neglected tropical diseases: where are we? Vaccine 31:40–48CrossRefPubMedGoogle Scholar
  32. Rukavtsova EB, Rudenko NV, Puchko EN et al (2015) Study of the immunogenicity of hepatitis B surface antigen synthesized in transgenic potato plants with increased biosafety. J Biotechnol 203:84–88CrossRefPubMedGoogle Scholar
  33. Ryan MD, Drew J (1994) Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J 13:928–933PubMedPubMedCentralCrossRefGoogle Scholar
  34. Sciutto E, Fragoso G, Fleury A et al (2000) Taenia solium disease in humans and pigs: an ancient parasitosis disease rooted in developing countries and emerging as a major health problem of global dimensions. Microbes Infect 2:1875–1890CrossRefPubMedGoogle Scholar
  35. Wales AD, Davies RH (2017) Salmonella vaccination in pigs: a review. Zoonoses Public Health 64:1–13CrossRefPubMedGoogle Scholar
  36. Walmsley AM, Arntzen CJ (2000) Plants for delivery of edible vaccines. Curr Opin Biotechnol 11:126–129CrossRefPubMedGoogle Scholar
  37. Wang L, Coppel RL (2008) Oral vaccine delivery: can it protect against non-mucosal pathogens? Expert Rev Vaccines 7:729–738. Scholar
  38. World Health Organization (2010) Working to overcome the global impact of neglected tropical diseases: first WHO report on neglected tropical diseases. Geneva, 172 pGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Edda Sciutto
    • 1
  • Marisela Hernández
    • 1
  • Jacquelynne Cervantes-Torres
    • 1
  • Elizabeth Monreal-Escalante
    • 1
  • Omayra Bolaños-Martínez
    • 1
  • Juan Francisco Rodríguez
    • 1
  • Gladis Fragoso
    • 1
  • Sergio Rosales-Mendoza
    • 2
    • 3
    Email author
  1. 1.Dpto. Inmunología. Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de México. Circuito Escolar. Ciudad UniversitariaMéxicoMexico
  2. 2.Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias QuímicasUniversidad Autónoma de San Luis PotosíSan Luis PotosíMexico
  3. 3.Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y BiomedicinaUniversidad Autónoma de San Luis PotosíSan Luis PotosíMexico

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