Stress-Induced Microspore Embryogenesis by Anther Culture of Quercus suber L.

  • Pilar S. TestillanoEmail author
  • Beatriz Pintos
  • Aranzazu Gomez-Garay
  • María C. Risueño
Part of the Forestry Sciences book series (FOSC, volume 84)


In vivo, the microspore inside the anther divides and follows the gametophytic program to form the mature pollen grain. In vitro, upon the application of a stress treatment the microspore can be deviated towards a proliferation process leading to embryogenesis, the so-called microspore embryogenesis that can be induced in anther and isolated microspore cultures (Maluszynski et al. 2003).



This work has been supported by projects (BFU2011-23752, AGL2014-52028-R, AGL2017-82447-R) funded by the Spanish Ministry of Economy and Competitiveness, MINECO, and the European Regional Development Fund (ERDF/FEDER) of the European Commission.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Pilar S. Testillano
    • 1
    Email author
  • Beatriz Pintos
    • 2
  • Aranzazu Gomez-Garay
    • 2
  • María C. Risueño
    • 1
  1. 1.Pollen Biotechnology of Crop Plants GroupBiological Research Centre, CIB-CSICMadridSpain
  2. 2.Department of Plant Biology I, Faculty of BiologyComplutense University of Madrid, UCMMadridSpain

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