Stress-Induced Microspore Embryogenesis by Anther Culture of Quercus suber L.
In vivo, the microspore inside the anther divides and follows the gametophytic program to form the mature pollen grain. In vitro, upon the application of a stress treatment the microspore can be deviated towards a proliferation process leading to embryogenesis, the so-called microspore embryogenesis that can be induced in anther and isolated microspore cultures (Maluszynski et al. 2003).
This work has been supported by projects (BFU2011-23752, AGL2014-52028-R, AGL2017-82447-R) funded by the Spanish Ministry of Economy and Competitiveness, MINECO, and the European Regional Development Fund (ERDF/FEDER) of the European Commission.
- Chiancone B, Karasawa MM, Gianguzzi V, Abdelgalel AM, Bárány I, Testillano PS, Marinoni DT, Botta R, Germanà MA (2015) Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’. Front Plant Sci 6:413CrossRefPubMedPubMedCentralGoogle Scholar
- Germanà MA (2009) Haploids and doubled haploids in fruit trees. In: Forster B, Jain M, Touraev A (eds) Advances in haploid production in higher plants. Springer, New York, pp 241–263Google Scholar
- Maluszynski M, Kasha K, Forster B, Szarejko I (eds) (2003) Doubled haploid production in crop plants: a manual. Kluwer, DordrechtGoogle Scholar
- Prem D, Solís MT, Bárány I, Rodríguez-Sánz H, Risueño MC, Testillano PS (2012) A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus. BMC Plant Biol 12:127CrossRefPubMedPubMedCentralGoogle Scholar
- Ramírez C, Chiancone B, Testillano PS, García-Fojeda B, Germanà MA, Risueño MC (2003) First embryogenic stages of Citrus microspore-derived embryos. Acta Biol Cracov Ser Bot 45:53–58Google Scholar