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SE Fluidics System

  • Cyrus K. Aidun
  • Ulrika Egertsdotter
Chapter
Part of the Forestry Sciences book series (FOSC, volume 84)

Abstract

Somatic embryogenesis (SE) offers a basis for scalable, automated technology suitable for large-scale production of clonal plants. The SE method is attractive biologically due to the developmental path of the somatic embryo closely resembling zygotic embryo development thus avoiding issues related to adventitious rooting and plagiotropic growth. Furthermore, long term storage of SE cultures allow for field testing in species where zygotic seeds are the starting material for SE cultures, e.g. conifers. Application of SE methods for industrial scale plant production has been limited due to the cost of labor involved with different steps of the SE process.

Notes

Acknowledgements

Several companies and research foundations have funded various parts of the development of the automation system. We acknowledge financial support from SweTree Technologies and it’s consortium of associated Swedish forest companies, VINNOVA Swedish innovation agency and the Kempe Foundations. We also acknowledge the support and hard work of all engineers and scientists involved with these projects.

References

  1. Aidun CK (2013) Fluidics-based orientation and sorting device for plant embryos. Assignee—Georgia Tech Research Corporation. Patent 8452460. Issued 28 May 2013Google Scholar
  2. Aidun CK (2015a) Methods for dispersing somatic plant embryos. Assignee—Georgia Tech Research Corporation. Patent 8452460. Issued 10 Mar 2015Google Scholar
  3. Aidun CK (2015b) Separator device, depostion device, and system for handling of somatic embryos. Assignee—Georgia Tech Research Corporation. Patent 9040301. Issued 26 May 2015Google Scholar
  4. Aidun CK (2015c) Planting method and device for plant propagules. Applicant—Georgia Tech Research Corporation. Patent publication no. WO/2015/097603. Published 2 July 2015Google Scholar
  5. Aidun CK (2016) Integrated germination method and device. Applicant—Georgia Tech Research Corporation. Patent publication no. WO2016098083 A1. Published 23 June 2016Google Scholar
  6. Aidun CK, Egertsdotter U (2012) Fluidics-based automation of clonal propagation via somatic embryogenesis: SE-fluidics system. In Proceedings of 2nd IUFRO working party, Bruno, Czech Republic, 2.09.02 25-8, June 2012Google Scholar
  7. Aidun CK, Egertsdotter U (2015) Method for dispersion of assemblies of biological material. Assignee—Georgia Tech Research Corporation, Patent 8927287. Issued 6 Jan 2015Google Scholar
  8. Aronen T, Egertsdotter U (2014) Close to application of somatic embryogenesis. SNS News Views 6:2014Google Scholar
  9. Businge E, Brackmann K, Moritz T, Egertsdotter U (2012) Metabolite profiling reveals clear metabolic changes during somatic embryo development of Norway spruce (Picea abies). Tree Physiol 32(2):232–244CrossRefPubMedGoogle Scholar
  10. Businge E, Bygdell J, Wingsle G, Moritz T, Egertsdotter U (2013) The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos. Physiol Plant 149(2):273–285CrossRefPubMedGoogle Scholar
  11. Businge E, Trifonova A, Schneider C, Rödel P, Egertsdotter U (2017) Evaluation of a new temporary immersion bioreactor system for micropropagation of cultivars of eucalyptus, birch and fir. Forests 8(6):196CrossRefGoogle Scholar
  12. Dobrowolska I, Businge E, Abreu IN, Moritz T, Egertsdotter U (2017) Metabolome and transcriptome profiling reveal new insights into somatic embryo germination in Norway spruce (Picea abies). Tree Physiol 00:1–15.  https://doi.org/10.1093/treephys/tpx078CrossRefGoogle Scholar
  13. Filonova LH, Bozhkov PV, von Arnold S (2000) Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-lapse tracking. J Exp Bot 51:249–264CrossRefPubMedGoogle Scholar
  14. Högberg K-A, Ekberg I, Norell L, von Arnold S (1998) Integration of somatic embryogenesis in a tree breeding programme: a case study with Picea abies. Can J Forest Res 28:1536–1545CrossRefGoogle Scholar
  15. Kong L, von Aderkas P (2011) A novel method of cryopreservation without a cryoprotectant for immature somatic embryos of conifer. Plant Cell, Tissue Organ Cult 106:115–125CrossRefGoogle Scholar
  16. Lelu-Walter M-A, Thompson D, Harvengt L, Sanchez L, Toribio M, Pâgues LE (2013) Somatic embryogenesis in forestry with a focus on Europe: state-of-the-art, benefits, challenges and future. Tree Genet. Genomes 9:883–899CrossRefGoogle Scholar
  17. Lu G, Fei B (2014) Medical hyperspectral imaging: a review. J Biomed Opt 19(1):010901CrossRefPubMedCentralGoogle Scholar
  18. Mamun NHA, Egertsdotter U, Aidun CK (2014) Bioreactor technology for clonal propagation of plants and metabolite production. Front Biol 10(2):177–193CrossRefGoogle Scholar
  19. Sharma S, Shahzad A, Teixeira da Silva JA (2013) Synseed technology—a complete synthesis. Biotech Adv 31:186–207CrossRefGoogle Scholar
  20. Swedish Statistical Yearbook of Forestry (2014) Skogsstyrelsen, Swedish Forest Agency, ISSN 0491-7847, ISBN 978-91-87535-05-5Google Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.G.W. Woodruff School of Mechanical Engineering, Parker H. Petit Institute for Bioengineering and Bioscience and Renewable Bioproducts InstituteGeorgia Institute of TechnologyAtlantaUSA
  2. 2.Department of Forest Genetics and Plant Physiology, Umeå Plant Science CentreSwedish Agricultural UniversityUmeåSweden

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