Protocol for Somatic Embryogenesis in Passiflora cincinnata Mast. (Passifloraceae)

  • Lorena Melo Vieira
  • Priscila Oliveira Silva
  • Amanda Mendes Fernandes
  • Diego Ismael Rocha
  • Wagner Campos Otoni
Part of the Forestry Sciences book series (FOSC, volume 85)


The process of somatic embryogenesis has become an essential asset, as it enables plant regeneration and large-scale propagation. Our research team has pioneered a reproducible protocol for somatic embryogenesis using mature zygotic embryo of P. cincinnata. Here, we describe in details the protocol for P. cincinnata, in which the explants were exposed to medium supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Due to the efficacy and reproducibility of this regeneration protocol, new perspectives arise as the protocol can be extended to other Passifloraceae species that arouse agronomic, ornamental and commercial interest. Moreover, this is an alternative for genetic transformation protocols, based up to now on the organogenic system.



The Brazilian sponsoring agencies Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, MG, Brazil; Grant no. CBB - APQ-01131-15) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil: Grant no. 459.529/2014-5) are acknowledged for the financial support. P.O.S. was recipient of a PhD scholarship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil). A.M.F. is currently recipient of a MSc scholarship from FAPEMIG, and L.M.V. a post-doctoral scholarship from CAPES/PNPD). Authors are also grateful to Dr. Daniela L. Paim-Pinto for generously making available Figs. 21.4B and 21.4C, and to Mr. Herculano José de Freitas, for kindly making available the photos in Fig. 21.1. Our gratitude to Caio G. Otoni for the English revision.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Lorena Melo Vieira
    • 1
  • Priscila Oliveira Silva
    • 1
  • Amanda Mendes Fernandes
    • 1
  • Diego Ismael Rocha
    • 2
  • Wagner Campos Otoni
    • 1
  1. 1.Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos—LCT, Instituto de Biotecnologia Aplicada à Agropecuária-BIOAGROUniversidade Federal de ViçosaViçosaBrazil
  2. 2.Instituto de BiociênciasUniversidade Federal de GoiásJataíBrazil

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