Somatic Embryogenesis of Date Palm (Phoenix dactylifera L.) from Shoot Tip Explants

  • Jameel M. Al-KhayriEmail author
Part of the Forestry Sciences book series (FOSC, volume 85)


Date palm (Phoenix dactylifera L.) is a fruit tree adapted the desert climate. The dates are rich in sugar, minerals, vitamins, and numerous pharmaceutical compounds. Seeds propagating results in heterogeneous offsprings with inferior dates. Therefore propagation of offshoots is the preferred method among farmers. This method, however, is constrained by the limited number of offshoots. Currently, micropropagation is used effectively for large-scale commercial propagation of date palm. This chapter describes a micropropagation protocol based on indirect somatic embryogenesis using apical shoot tip explants. Fully-detailed step-wise procedures are described including explant isolation and disinfection, callus induction and maintenance, development of somatic embryos, rooting and plantlet formation, and finally acclimatization and field establishment.


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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Agricultural Biotechnology, College of Agriculture and Food SciencesKing Faisal UniversityAl-HassaSaudi Arabia

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