Treponema pallidum Hemagglutination (TPHA) Test

  • Rowa Yousef Alhabbab
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Syphilis is a sexually transmitted disease caused by bacteria called Treponema pallidum, and range from asymptomatic to severe. Patients with suspected syphilis should undergo a non-treponemal screening test (rapid plasma reagin) and positive results must be confirmed with a test specific to T. pallidum antigen. Here, the treponemal test, T. pallidum hemagglutination test, principle, reagents required, steps, and results interpretation in addition to examples to clarify the results interpretation process are described.


Syphilis Treponema pallidum Treponemal test TPHA Hemagglutination 


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

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