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Immunofluorescence (IF) Assay

  • Rowa Yousef Alhabbab
Chapter
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)

Abstract

Immunofluorescence (IF) assay is largely used in clinical immunology laboratories, especially in autoimmune cases because it is a method that provides histological demonstration of antibodies and antigens related to the pathogenesis of the disease. This chapter covers the principles of the different IF test types, in addition to their reagents, steps, and results interpretation.

Keywords

Immunofluorescence IF Autoimmune Direct IF Indirect IF Indirect complement fixation IF-CF Double immunofluorescence DIF 

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

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