Immunofixation Electrophoresis (IFE)

  • Rowa Yousef Alhabbab
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Immunofixation electrophoresis (IFE) is a fundamental technique for detecting and quantifying plasma cell disorders such as monoclonal gammopathies. Samples with abnormal proteins such as M protein on the electrophoresis gel have to be confirmed and characterized by IFE. The principle, reagents, steps, and results interpretation of IFE are explained here.


IFE M protein Monoclonal gammopathies MGUS Agarose gel Cellulose Acetate membrane 


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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

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