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Structure of Yeast Nuclear Pore Complexes

  • Lan Mi
  • Longfang Yao
  • Jiong Ma
Chapter
Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 33)

Abstract

Nuclear pore complexes (NPCs) are large protein complex assemblies by about 30 different proteins, called nucleoporins (Nups), embedded in the nuclear envelope. Most of transport of molecules between cytoplasm and nucleus occurs through the NPCs. The research of yeast and vertebrate NPC structure made big progress in the past decades. This chapter first reviews recent advances of NPC structure and architecture by electron microscopy and super-resolution and then further overviews the progress of NPC structure and dynamic in living yeast cells by a single molecular detection approach called single-point edge-excitation sub-diffraction (SPEED) microscopy. In the last section, we will discuss the perspective about the structure of yeast NPCs.

Notes

Acknowledgments

This work is supported by National Natural Science Foundation of China (61575046, 11574056, and 31500599) and Science and Technology Commission of Shanghai Municipality (Shanghai Rising-Star Program, 16QA1400400).

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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Optical Science and EngineeringShanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Green Photoelectron Platform, Fudan UniversityShanghaiChina

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