Spermatogenic Stem Cell Cryopreservation and Transplantation
It was first shown by Ralph Brinster that spermatogenic stem cells derived from testis tissue of normal mice can be transferred via the rete testis into mice with no spermatogenesis, and they will gradually populate stem cell niches all along the seminiferous tubules of large areas of the previously sterile recipient mouse testis and result in normal spermatogenesis and ultimately in normal offspring (Fig. 18.1) [1, 2]. Subsequently spermatogenic stem cells from a variety of species were successfully transplanted into SCID mice. The closer the phylum of the donor spermatogenic stem cells to the sterilized recipient mouse, the farther along the spermatogenesis of the transplanted germ cells would develop (Fig. 18.2). For example, rat stem cells would develop mature sperm in the SCID mouse but at the rate of rat spermatogenesis rather than mouse spermatogenesis. For more distant phyla, the final stages of spermatogenesis are not seen although earlier stages are well supported [3–8]. Frozen spermatogenic stem cells did just as well as fresh .
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