Advertisement

Indian Spices pp 405-419 | Cite as

Plant Tissue Culture as Potential Option in Developing Climate Resilient Spices

  • Md. Nasim Ali
  • Syandan Sinha Ray
Chapter

Abstract

According to, Food and Drugs Administration spices are “Aromatic vegetable substance, in the whole, broken, or ground form, whose significant function in food is seasoning rather than nutrition”. Spices have gained a significant position in modern life due to its economic importance and its cuisine, food and medicinal value. In modern era of civilization, global warming and climate changes are becoming a major threat for all living organisms of the globe including spices. Despite the tremendous progress and mechanization in the agriculture sector, it is also experiencing the multidimensional negative impact of climate changes. The natural resources of spices are declining day by day due to deforestation and industrialization. Besides, low productivity, seasonal dependency, high susceptible to diseases and poor genetic variation are major problems for spices cultivation. So production and supply of quality products is becoming a great challenge. In addition, there is a need to develop climate resilient crops in order to face the consequences of global warming in near future. Plant Tissue Culture technology is a proven techniques for generating quality planting materials and developing new elite germplasms of several crops. The regeneration of planting materials by this technique depends on major factors of plant tissue culture methods are type of explants, culture media and culture condition. Modification of media components and using suitable explants under stress conditions, several stress-tolerant/resistant crops have been regenerated through this technique. This chapter focuses to enumerate applicability and scope in the improvement of spices with response to climatic change. Major aspects of plant tissue culture for spices improvement are mainly restricted to somatic embryogenesis, protoplast fusion (i.e. cybrid) while disease free plants can be regenerated through micropropagation. Anther/pollen culture was exclusively reported for haploid/double haploid plant production. Somaclonal variations regenerated through plant tissue culture exhibited the scope to induce variation under laboratory condition. Moreover, secondary metabolites enhancement particularly in spices with pharmaceutical importance and long term conservation of rare germplasms are the areas of intrusion. Since traditional breeding is laborious and time taking process, plant tissue culture may serve as chief tool or as an inseparable add-on tool to recombinant DNA technonology.

Keywords

Crop improvement Climate change Plant tissue culture Spices Somaclones 

References

  1. Adaniya S, Shirai D (2001) In vitro induction of tetraploid ginger (Zingiber officinale roscoe) and its pollen fertility and germinability. Sci Hortic 88(4):277–287CrossRefGoogle Scholar
  2. Ahmad S, Garg M, Tamboli ET, Abdin MZ, Ansari SH (2013) In vitro production of alkaloids: factors, approaches, challenges and prospects. Pharm Rev 7(13):27–33CrossRefGoogle Scholar
  3. Aitken-Christie J, Kozai T, Takayama S (1995) Automation in plant tissue culture—general introduction and overview. In Automation and environmental control in plant tissue culture. Springer, Netherlands, pp 1–18CrossRefGoogle Scholar
  4. Altman A (2003) From plant tissue culture to biotechnology: scientific revolutions, abiotic stress tolerance, and forestry. In Vitro Cell Dev Biol – Plant 39:75.  https://doi.org/10.1079/IVP2002379 CrossRefGoogle Scholar
  5. Anasori P, Asghari G (2009) Effects of light and differentiation on gingerol and zingiberene production in callus culture of Zingiber officinale Rosc. Res Pharm Sci 3(1):59–63Google Scholar
  6. Angelova Z, Georgiev S, Roos W (2006) Elicitation of plants. Biotechnol and Biotechnol Eq 20:72–83CrossRefGoogle Scholar
  7. Arora DS, Kaur J (1999) Antimicrobial activity of spices. Int J Antimicrob Agents 12(3):257–262CrossRefGoogle Scholar
  8. Babu KN, Samsudeen K, Ratnambal MJ, Ravindran PN (1996) Embryogenesis and plant regeneration from ovary derived callus cultures of ginger (Zingiber officinale rose.) J Spices Aromatic Crops 5(2):134–138Google Scholar
  9. Babu KN, Geetha SP, Divakaran M, Ravindran PN, Peter KV (1999) In vitro conservation of cardamom (Elettaria cardamom Maton) germplasm. Plant Genet Resour Newsl 119:41–45Google Scholar
  10. Babu KN, Yamuna G, Praveen K, Divakaran M, Ravindran PN, Peter KV (2012) Cryopreservation of spices genetic resources. In Igor I. Katkov (ed). Current Frontiers in Cryobiology, InTech-Open Access Publisher. (Croatia) p 457–484Google Scholar
  11. Bajpai A, Kalim S, Chandra R, Kamle M (2016) Recurrent somatic embryogenesis and plantlet regeneration in Psidium guajava L. Braz Arch Biol Technol 59(1):1–12Google Scholar
  12. Balachandran SM, Bhat SR, Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc). Plant Cell Rep 8(9):521–524CrossRefGoogle Scholar
  13. Ballester A, Janeiro LV, Vieitez AM (1997) Cold storage of shoot cultures and alginate encapsulation of shoot tips of Camellia japonica L. and Camellia reticulata Lindley. Sci Hortic 71(1–2):67–78CrossRefGoogle Scholar
  14. Bates GW, Gaynor JJ, Shekhawat NS (1983) Fusion of plant protoplasts by electric fields. Plant Physiol 72(4):1110–1113CrossRefGoogle Scholar
  15. Beckford CL, Norman A (2016) Climate change and quality of planting materials for domestic food production: tissue culture and protected agriculture. In: Beckford C, Rhiney K (eds) Globalization, agriculture and food in the Caribbean. Palgrave Macmillan, LondonCrossRefGoogle Scholar
  16. Bhagyalakshmi B, Singh NS (1988) Meristem culture and micropropagation of a variety of ginger (Zingiber officinale Rosc.) with a high yield of oleoresin. J Hortic Sci 63(2):321–327CrossRefGoogle Scholar
  17. Bhat SR, Chandel KPS, Malik SK (1995) Plant regeneration from various explants of cultivated Piper species. Plant Cell Rep 14(6):398–402CrossRefGoogle Scholar
  18. Carlson PS, Smith HH, Dearing RD (1972) Parasexual interspecific plant hybridization. Proc Natl Acad Sci 69(8):2292–2294CrossRefGoogle Scholar
  19. Cervelli R, Senaratna T (1995) Economic aspects of somatic embryogenesis. Kluwer Academic Publishers, Dordrecht, pp 29–64Google Scholar
  20. Chaudhury R, Malik SK (2004) Genetic conservation of plantation crops and spices using cryopreservation. Indian J Biotechnol 3(3):348–358Google Scholar
  21. Cheng Y, Ma RL, Jiao YS, Qiao N, Li TT (2013) Impact of genotype, plant growth regulators and activated charcoal on embryogenesis induction in microspore culture of pepper (Capsicum annuum L). S Afr J Bot 88:306–309CrossRefGoogle Scholar
  22. Cocking EC (1960) A method for the isolation of plant protoplasts and vacuoles. Nature 187(4741):962–963CrossRefGoogle Scholar
  23. Croteau R, Kutchan TM, Lewis NG (2000) Natural products (secondary metabolites). Biochem Mol Biology Plants 24:1250–1319Google Scholar
  24. Durieu P, Ochatt SJ (2000) Efficient intergeneric fusion of pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.) protoplasts. J Exp Bot 51(348):1237–1242Google Scholar
  25. Ebrahimie E, Habashi AA, Ghareyazie B, Ghannadha M, Mohammadie M (2003) A rapid and efficient method for regeneration of plantlets from embryo explants of cumin (Cuminum cyminum). Plant Cell Tissue Organ Cult 75(1):19–25CrossRefGoogle Scholar
  26. Ercan N, Sensoy FA, Sensoy AS (2006) Influence of growing season and donor plant age on anther culture response of some pepper cultivars (Capsicum annuum L). Sci Hortic 110(1):16–20CrossRefGoogle Scholar
  27. Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tissue Organ Cult 80(2):187–190CrossRefGoogle Scholar
  28. Faria RT, Illg RD (1995) Micropropagation of Zingiber spectabile Griff. Sci Hortic 62(1–2):135–137CrossRefGoogle Scholar
  29. Forster BP, Heberle-Bors E, Kash KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12(8):368–375CrossRefGoogle Scholar
  30. Gamborg OL, Miller R, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50(1):151–158CrossRefGoogle Scholar
  31. Gaspar T, Kevers C, Penel C, Greppin H, Reid DM, Thorpe TA (1996) Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cel Develop Biology-Plant 32(4):272–289CrossRefGoogle Scholar
  32. Gautheret RJ (1934) Culture du tissus cambial. Comptes Rendus Hebdomadaires des Se’ances de l’Acade’mie des. Science 198:2195–2196Google Scholar
  33. Geetha SP, Babu KN, Rema J, Ravindran PN, Peter KV (2000) Isolation of protoplasts from cardamom (Elettaria cardamomum Maton.) and ginger (Zingiber officinale rose.) J Spices Aromatic Crops 9(1):23–30Google Scholar
  34. George EF, Hall MA, De Klerk GJ (2008) Plant tissue culture procedure-background. In: Plant propagation by tissue culture. Springer, Netherlands, pp 1–28Google Scholar
  35. Germanà MA (2011) Anther culture for haploid and doubled haploid production. Plant Cell Tissue Organ Cult 104(3):283–300CrossRefGoogle Scholar
  36. Gonzalez-Arnao MT, Lazaro-Vallejo CE, Engelmann F, Gamez-Pastrana R, Martinez-Ocampo YM, Pastelin-Solano MC, Diaz-Ramos C (2009) Multiplication and cryopreservation of vanilla (vanilla Planifolia ‘Andrews)’. In: Vitro Cell Dev Biology–Plant 45(5):574–582Google Scholar
  37. Guan Q, Guo Y, Wei Y, Meng F, Zhang Z (2010) Regeneration of somatic hybrids of ginger via chemical protoplast fusion. Plant Cell Tissue Organ Cult 102(3):279–284CrossRefGoogle Scholar
  38. Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497CrossRefGoogle Scholar
  39. Guo Y, Bai J, Zhang Z (2007) Plant regeneration from embryogenic suspension-derived protoplasts of ginger (Zingiber officinale Rosc). Plant Cell Tissue Organ Cult 89(2–3):151–157CrossRefGoogle Scholar
  40. Hall RD, Holden MA, Yeoman MM (1987) The accumulation of phenylpropanoid and capsaicinoid compounds in cell cultures and whole fruit of the chilli pepper, Capsicum frutescens mill. Plant Cell Tissue Organ Cult 8(2):163–176CrossRefGoogle Scholar
  41. Hamirah MN, Sani HB, Boyce PC, Sim SL (2010) Micropropagation of red ginger (Zingiber montanum Koenig), a medicinal plant. Asia-Pacific J Mol Biol Biotechnol 18(1):127–130Google Scholar
  42. Hussain A, Na S, Nazir H, Shinwari ZK (2011) Tissue culture of black pepper (Piper nigrum L.) in Pakistan. Pak J Bot 43(2):1069–1078Google Scholar
  43. Ibrahim DA, Danial GH, Mosa VM, Khalil BM (2015) Plant regeneration from shoot tips-derived callus of ginger (Zingiber officinale Rosc.) Am J ExpAgric 7(1):55–61Google Scholar
  44. Ilahi IHSAN, Jabeen M (1992) Tissue culture studies for micropropagation and extraction of essential oils from Zingiber officinale Rosc. Pak J Bot 24:54–54Google Scholar
  45. Ilyas M (1976) Spices in India. Econ Bot 30(3):273–280CrossRefGoogle Scholar
  46. Irikova T, Grozeva S, Rodeva V (2011) Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiol Plant 33(5):1559–1570CrossRefGoogle Scholar
  47. Islam MT, Leunufna S, Dembele DP, Keller EJ (2003) In vitro conservation of four mint (Mentha spp.) accessions. Plant Tissue Culture 13:37–46Google Scholar
  48. Jain SC, Pancholi B, Jain R (2012) In-vitro callus propagation and secondary metabolite quantification in Sericostoma pauciflorum. Iranian J Pharm Res: IJPR 11(4):1103–1109Google Scholar
  49. Kaefer CM, Milner JA (2008) The role of herbs and spices in cancer prevention. J Nutr Biochem 19(6):347–361CrossRefGoogle Scholar
  50. Kavyashree R (2009) An efficient in vitro protocol for clonal multiplication of ginger–var. Varada. Indian J Biotechnol 8(3):328–331Google Scholar
  51. Khanpour-Ardestani N, Sharifi M, Behmanesh M (2015) Establishment of callus and cell suspension culture of Scrophularia striata Boiss.: an in vitro approach for acteoside production. Cytotechnology 67(3):475–485CrossRefGoogle Scholar
  52. Koleva-Gudeva L, Trajkova F, Dimeska G, Spasenoski M (2008) Androgenesis efficiency in anther culture of pepper (Capsicum annuum L.). In: IV Balkan Symposium on Vegetables and Potatoes 830, p 183–190Google Scholar
  53. Kunnumakkara AB, Koca C, Dey S, Gehlot P, Yodkeeree S, Danda D, Sung B, Aggarwal BB (2009) Traditional uses of spices: an overview. Molecular targets and therapeutic uses of spices. World Scientific Publishing Co. Pte. Ltd, Singapore, pp 1–24CrossRefGoogle Scholar
  54. Lainé E, David A (1994) Regeneration of plants from leaf explants of micropropagated clonal Eucalyptus grandis. Plant Cell Rep 13(8):473–476CrossRefGoogle Scholar
  55. Lantos C, Juhász AG, Vági P, Mihály R, Kristóf Z, Pauk J (2012) Androgenesis induction in microspore culture of sweet pepper (Capsicum annuum L.) Plant Biotechnol Rep 6(2):123–132CrossRefGoogle Scholar
  56. Larkin PJ, Scowcroft SC (1981) Somaclonal variation-a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60:197–121CrossRefGoogle Scholar
  57. Luitel BP, Kang WH (2013) In vitro androgenic response of minipaprika (Capsicum annuum L.) genotypes in different culture media. Hortic Environ Biotechnol 54(2):162–171CrossRefGoogle Scholar
  58. Maheswaran G, Williams EG (1984) Direct somatic embryoid formation on immature embryos of Trifolium repens, T. pratense and Medicago sativa, and rapid clonal propagation of T. repens. Ann Bot 54(2):201–212CrossRefGoogle Scholar
  59. Maiti CS, Yepthomi GI (2015) In vitro multiplication for disease free healthy seed rhizome production of ginger (Zingiber officinale). IJB 1(4):5–12Google Scholar
  60. Mathew RE, Sankar PD (2014) Comparison of major secondary metabolites quantified in elicited cell cultures, non-elicited cell cultures, callus cultures and field grown plants of Ocimum. Int J Pharm Pharm Sci 6:102–106Google Scholar
  61. Morel G, Martin C (1952) Gue’rison de dahlias attaints d’une maladie a´ virus. Comptes Rendus Hebdomadaires des Se’ances de l’Acade’mie des. Science 235:1324–1325Google Scholar
  62. Mulabagal V, Tsay HS (2004) Plant cell cultures-an alternative and efficient source for the production of biologically important secondary metabolites. Int J Appl Sci Eng 2(1):29–48Google Scholar
  63. Munyon IP, Hubstenberger JF, Phillips GC (1989) Origin of plantlets and callus obtained from chile pepper anther cultures. In Vitro Cell Dev Biology 25(3):293–296CrossRefGoogle Scholar
  64. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497CrossRefGoogle Scholar
  65. Murthy HN, Lee EJ, Paek KY (2014) Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tissue Organ Cult 118(1):1–16CrossRefGoogle Scholar
  66. Nair RR, Gupta SD (2006) High-frequency plant regeneration through cyclic secondary somatic embryogenesis in black pepper (Piper nigrum L.) Plant Cell Rep 24(12):699–707CrossRefGoogle Scholar
  67. Nayak S, Naik PK (2006) Factors effecting in vitro microrhizome formation and growth in Curcuma longa L. and improved field performance of micropropagated plants. Sci Asia 32:31–37CrossRefGoogle Scholar
  68. Olszewska D, Kisiała A, Nowaczyk P (2011) The assessment of doubled haploid lines obtained in pepper (Capsicum annuum L.) anther culture. Folia Horticulturae 23(2):93–99CrossRefGoogle Scholar
  69. Pandey RY, Sagwansupyakorn C, Sahavacharin O, Thaveechai N (1997) In vitro propagation of ginger (Zingiber officinale roscoe). Kasetsart J (Nat Sci) 31(1):81–86Google Scholar
  70. Patel K, Srinivasan K (2004) Digestive stimulant action of spices: a myth or reality. Indian J Med Res 119:167–179Google Scholar
  71. Peter KV, Babu NK, Minoo D (2006) Spices biotechnology. J Hortic Sci 1(1):1–14Google Scholar
  72. Philip VJ, Nainar SAZ (1986) Clonal propagation of Vanilla planifolia (Salisb.) Ames using tissue culture. J Plant Physiol 122(3):211–215CrossRefGoogle Scholar
  73. Philip VJ, Joseph D, Triggs GS, Dickinson NM (1992) Micropropagation of black pepper (Piper nigrum Linn.) through shoot tip cultures. Plant Cell Rep 12(1):41–44CrossRefGoogle Scholar
  74. Pierik RLM (1991) Micropropagation of ornamental plants. Acta Hortic 289:45–53CrossRefGoogle Scholar
  75. Prutpongse P, Gavinlertvatana P (1992) In vitro micropropagation of 54 species from 15 genera of bamboo. Hortscience 27(5):453–454Google Scholar
  76. Rahman MM, Amin MN, Jahan HS, Ahmed R (2004) In vitro regeneration of plantlets of Curcuma longa Linn. A valuable spice plant in Bangladesh. Asian J Plant Sci 3(3):306–309CrossRefGoogle Scholar
  77. Rai M, Kalia R, Singh R, Gangola MP, Dhawan A (2011) Developing stress-tolerant plants through in vitro selection—an overview of the recent progress. Environ Exp Bot 71:89–98.  https://doi.org/10.1016/j.envexpbot.2010.10.021 CrossRefGoogle Scholar
  78. Ramawat KG, Sharma R, Soni SS (2000) Medicinal plants. In: Ramawat KG, Merillon JM (eds) Biotechnology: secondary metabolites. IBH Publishing Co, Pvt. Ltd, Oxford, pp 356–356Google Scholar
  79. Rao PL (1996) Plant biotechnology: promises and challenges. Def Sci J 46(1):31–39CrossRefGoogle Scholar
  80. Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20(2):101–153CrossRefGoogle Scholar
  81. Rathore MS, Shekhawat NS (2008) Incredible spices of India: from traditions to cuisine. Am-Eurasian J Botany 1(3):85–89Google Scholar
  82. Roopadarshini V, Gayatri MC (2012) Isolation of somaclonal variants for morphological and biochemical traits in Curcuma longa (turmeric). Res Plant Biology 2(3):31–37Google Scholar
  83. Rout GR, Das P (1997) In vitro organogenesis in ginger (Zingiber officinale Rosc.) J Herbs, Spices Med Plants 4(4):41–51CrossRefGoogle Scholar
  84. Rout GR, Palai SK, Samantaray S, Das P (2001) Effect of growth regulator and culture conditions on shoot multiplication and rhizome formation in ginger (Zingiber officinale Rosc.) in vitro. In Vitro Cel Develop Biology-Plant 37(6):814–819CrossRefGoogle Scholar
  85. Rout GR, Mohapatra A, Jain SM (2006) Tissue culture of ornamental pot plant: a critical review on present scenario and future prospects. Biotechnol Adv 24(6):531–560CrossRefGoogle Scholar
  86. Samsudeen K, Babu KN, Divakaran M, Ravindran PN (2000) Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.) J Hortic Sci Biotechnol 75(4):447–450CrossRefGoogle Scholar
  87. Sato H, Enomoto S, Oka S, Hosomi K, Ito Y (1993) Plant regeneration from protoplasts of peppermint (Mentha piperita L). Plant Cell Rep 12(10):546–550CrossRefGoogle Scholar
  88. Sato H, Yamada K, Mii M, Hosomi K, Okuyama S, Uzawa M, Ishikawa U, Ito Y (1996) Production of an interspecific somatic hybrid between peppermint and gingermint. Plant Sci 115(1):101–107CrossRefGoogle Scholar
  89. Sharma TR, Singh BM (1997) High-frequency in vitro multiplication of disease-free Zingiber officinale Rosc. Plant Cell Rep 17(1):68–72CrossRefGoogle Scholar
  90. Singh SR, Singh R, Kalia S, Dalal S, Dhawan AK, Kalia RK (2013) Limitations, progress and prospects of application of biotechnological tools in improvement of bamboo—a plant with extraordinary qualities. Physiol Mol Biol Plants 19(1):21–41CrossRefGoogle Scholar
  91. Srinivasan K (2005) Role of spices beyond food flavoring: nutraceuticals with multiple health effects. Food Rev Int 21(2):167–188CrossRefGoogle Scholar
  92. Subbarayudu S, Naik BS, Devi HS, Bhau BS, Khan PSSV (2014) Microsporogenesis and pollen formation in Zingiber officinale roscoe. Plant Syst Evol 300(4):619–632CrossRefGoogle Scholar
  93. Sundararaj SG, Agrawal A, Tyagi RK (2010) Encapsulation for in vitro short-term storage and exchange of ginger (Zingiber officinale Rosc.) germplasm. Sci Hortic 125(4):761–766CrossRefGoogle Scholar
  94. Supena ED, Suharsono S, Jacobsen E, Custers JB (2006) Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.) Plant Cell Rep 25:1–10CrossRefGoogle Scholar
  95. Takebe I, Labib G, Melchers G (1971) Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 58(6):318–320CrossRefGoogle Scholar
  96. Tefera W, Wannakrairoj S (2004) Micropropagation of krawan (Amomum krervanh Pierre ex Gagnep). Sci Asia 30:9–15CrossRefGoogle Scholar
  97. Thiaman KV (1974) Fifty years of plant hormone research. Plant Physiol 54:450–453CrossRefGoogle Scholar
  98. Thorpe TA (2007) History of plant tissue culture. Mol Biotechnol 37(2):169–180CrossRefGoogle Scholar
  99. Tyagi RK, Yusuf A, Dua P, Agrawal A (2004) In vitro plant regeneration and genotype conservation of eight wild species of curcuma. Biol Plant 48(1):129–132CrossRefGoogle Scholar
  100. Tyagi RK, Agrawal A, Mahalakshmi C, Hussain Z, Tyagi H (2007) Low-cost media for in vitro conservation of turmeric (Curcuma longa L.) and genetic stability assessment using RAPD markers. In Vitro Cell Develop Biology–Plant 43(1):51–58CrossRefGoogle Scholar
  101. Tyagi RK, Goswami R, Sanayaima R, Singh R, Tandon R, Agrawal A (2009) Micropropagation and slow growth conservation of cardamom (Elettaria cardamomum Maton). In Vitro Cel Develop Biology-Plant 45(6):721–729CrossRefGoogle Scholar
  102. Villalobos VM, Engelmann F (1995) Ex situ conservation of plant germplasm using biotechnology. World J Microbiol Biotechnol 11(4):375–382CrossRefGoogle Scholar
  103. Wang LH, Zhang BX (2001) Advancement in the anther culture of Capsicum annuum L. China Veg 3:52–53Google Scholar
  104. Yamuna G, Sumathi V, Geetha SP, Praveen K, Swapna N, Nirmal Babu K (2007) Cryopreservation of in vitro grown shoots of ginger (Zingiber officinale Rosc.) CryoLetters 28(4):241–252Google Scholar
  105. Yasodha R, Sumathi R, Gurumurthi K (2004) Micropropagation for quality propagule production in plantation forestry. Indian J Biotechnol 3(2):159–170Google Scholar
  106. Yunus MF, Aziz MA, Kadir MA, Rashid AA (2012) In vitro propagation of Etlingera elatior (Jack)(torch ginger). Sci Hortic 135:145–150CrossRefGoogle Scholar
  107. Yusuf A, Tyagi RK, Malik SK (2001) Somatic embryogenesis and plantlet regeneration from leaf segments of Piper colubrinum. Plant Cell Tissue Organ Cult 65(3):255–258CrossRefGoogle Scholar
  108. Zhong JJ (2001) Biochemical engineering of the production of plant-specific secondary metabolites by cell suspension cultures. Plant Cell 72:1–26CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Md. Nasim Ali
    • 1
  • Syandan Sinha Ray
    • 2
  1. 1.Department of Agricultural Biotechnology, Faculty of AgricultureBidhan Chandra Krishi Viswavidyalaya, MohanpurNadiaIndia
  2. 2.Department of Agricultural BiotechnologySchool of Agriculture and Rural Development, Faculty Centre for Integrated Rural Development and Management, Ramakrishna Mission Vivekananda Educational and Research InstituteKolkataIndia

Personalised recommendations