Mimicking Physiological Oxygen in Cell Cultures

  • Nicholas R. ForsythEmail author
  • Rachel Steeg
  • Muhammad Ahmad
  • Mohammed Al Zubaidi
  • Rakad Al-Jumaily
  • Marwan Merkhan
  • Tina Dale
Part of the Learning Materials in Biosciences book series (LMB)


This chapter will provide a concise overview of the history of mammalian in vitro cell culture from its first descriptions through to the modern era. A specific focus will be adopted to understand when the importance of oxygen control emerged within the field, and we will discuss some of the earliest examples. A description of the common and state-of-the-art systems designed to provide an approximation of physiological normoxia will follow before finally describing its application in the emerging field of stem cell biology, specifically pluripotent stem cells and bone marrow-derived mesenchymal stem cells.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Nicholas R. Forsyth
    • 1
    Email author
  • Rachel Steeg
    • 2
  • Muhammad Ahmad
    • 1
  • Mohammed Al Zubaidi
    • 1
  • Rakad Al-Jumaily
    • 1
  • Marwan Merkhan
    • 1
  • Tina Dale
    • 1
  1. 1.Guy Hilton Research Centre, Institute of Science and Technology in Medicine, Keele UniversityStoke on TrentUK
  2. 2.Censo Biotechnologies, Roslin BiocentreRoslinUK

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