Essentials in Cell Culture
When starting to work in mammalian cell culture, it is essential to understand the major principles of in vitro cell cultivation. These principles include knowledge of basic cell biology, working under aseptic conditions, choosing the right cell lines, culture vessels and equipment, as well as general acquaintance with the existing methods for cell state monitoring. Even before crossing the lab door for the first time, one should already be armed with basic knowledge about cell growth and the processes behind it, as well as be familiar with the techniques to influence it. It must be said beforehand that there are no absolutely standard/uniform cell culture labs around the world. Equipment, plastic ware, and cell types are used depending on the final application. This can range from the specified production of valuable biomolecules to in vitro testing and basic research. In this chapter, a short history of cell culture development will be presented first. Afterward, we will cover the different aspects of modern mammalian cell culture including the variety of cell culture applications, the basics of working under aseptic conditions, cultivation vessels, cell growth kinetics, and the evaluation of cell cultures.
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